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Provedor de dados:  BABT
País:  Brazil
Título:  Phytase-Producing Bacteria from Extreme Regions in Indonesia
Autores:  Sajidan,
Wulandari,Rita
Sari,Evy Novita
Ratriyanto,Adi
Weldekiros,Hailu
Greiner,Ralf
Data:  2015-10-01
Ano:  2015
Palavras-chave:  16S rDNA
Bacillus sp. bacterial phytase phytate phytate-degrading enzyme
Resumo:  ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal conditions for phytate dephosphorylation: pH 4.0 and 50°C (isolate D10), pH 5.0 and 60°C (isolate MC6, and isolate MS5), pH 6.0 and 50°C (isolate D16) and pH 6.0 and 60°C (isolate D6) and pH 6.0 and 40°C (isolate MC8). Zn2+ and Fe3+ strongly inhibited phytate dephosphorylation with all phytase preparations studied. In the presence of Ca2+, an increase in phytase activity of 10-15% was obtained.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132015050173
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.58 n.5 2015
Direitos:  info:eu-repo/semantics/openAccess
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