| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose
|
| Autores: |
Pereira Júnior,José Antônio de Sousa
Correia,Marcos José
Oliveira,Neiva Tinti de
|
| Data: |
2003-06-01
|
| Ano: |
2003
|
| Palavras-chave: |
Lentinula edodes
Cellulase
Liquid medium cultivation
|
| Resumo: |
Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC, the maximum activity attained was 200 UI/mg protein only.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300004
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132003000300004
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.46 n.3 2003
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
