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Provedor de dados:  BJMBR
País:  Brazil
Título:  Rescuing axons from degeneration does not affect retinal ganglion cell death
Autores:  de Lima,S.
Mietto,B.S.
Paula,C.
Muniz,T.
Martinez,A.M.B.
Gardino,P.F.
Data:  2016-01-01
Ano:  2016
Palavras-chave:  Optic nerve crush
Calpain inhibitor
Axon preservation
Axon degeneration
Retinal ganglion cells
Cell survival
Resumo:  After a traumatic injury to the central nervous system, the distal stumps of axons undergo Wallerian degeneration (WD), an event that comprises cytoskeleton and myelin breakdown, astrocytic gliosis, and overexpression of proteins that inhibit axonal regrowth. By contrast, injured neuronal cell bodies show features characteristic of attempts to initiate the regenerative process of elongating their axons. The main molecular event that leads to WD is an increase in the intracellular calcium concentration, which activates calpains, calcium-dependent proteases that degrade cytoskeleton proteins. The aim of our study was to investigate whether preventing axonal degeneration would impact the survival of retinal ganglion cells (RGCs) after crushing the optic nerve. We observed that male Wistar rats (weighing 200-400 g; n=18) treated with an exogenous calpain inhibitor (20 mM) administered via direct application of the inhibitor embedded within the copolymer resin Evlax immediately following optic nerve crush showed a delay in the onset of WD. This delayed onset was characterized by a decrease in the number of degenerated fibers (P<0.05) and an increase in the number of preserved fibers (P<0.05) 4 days after injury. Additionally, most preserved fibers showed a normal G-ratio. These results indicated that calpain inhibition prevented the degeneration of optic nerve fibers, rescuing axons from the process of axonal degeneration. However, analysis of retinal ganglion cell survival demonstrated no difference between the calpain inhibitor- and vehicle-treated groups, suggesting that although the calpain inhibitor prevented axonal degeneration, it had no effect on RGC survival after optic nerve damage.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400705
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/1414-431X20155106
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.49 n.4 2016
Direitos:  info:eu-repo/semantics/openAccess
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