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Provedor de dados:  BJM
País:  Brazil
Título:  Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil
Autores:  Viramontes-Ramos,Sabina
Portillo-Ruiz,Martha Cristina
Ballinas-Casarrubias,María de Lourdes
Torres-Muñoz,José Vinicio
Rivera-Chavira,Blanca Estela
Nevárez-Moorillón,Guadalupe Virginia
Data:  2010-10-01
Ano:  2010
Palavras-chave:  Biosurfactants
Bioemulsifiers
Soil microbiota
Hydrocarbon contamination
Resumo:  Petroleum-derived hydrocarbons are among the most persistent soil contaminants, and some hydrocarbon-degrading microorganisms can produce biosurfactants to increase bioavailability and degradation. The aim of this work was to identify biosurfactant-producing bacterial strains isolated from hydrocarbon-contaminated sites, and to evaluate their biosurfactant properties. The drop-collapse method and minimal agar added with a layer of combustoleo were used for screening, and positive strains were grown in liquid medium, and surface tension and emulsification index were determined in cell-free supernantant and cell suspension. A total of 324 bacterial strains were tested, and 17 were positive for the drop-collapse and hydrocarbon-layer agar methods. Most of the strains were Pseudomonas, except for three strains (Acinetobacter, Bacillus, Rhodococcus). Surface tension was similar in cell-free and cell suspension measurements, with values in the range of 58 to 26 (mN/m), and all formed stable emulsions with motor oil (76-93% E24). Considering the variety of molecular structures among microbial biosurfactants, they have different chemical properties that can be exploited commercially, for applications as diverse as bioremediation or degradable detergents.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300017
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822010000300017
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.41 n.3 2010
Direitos:  info:eu-repo/semantics/openAccess
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