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Provedor de dados:  BJM
País:  Brazil
Título:  Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using immobilized cells
Autores:  Orsi,Daniela C.
Kawaguti,Haroldo Y.
Sato,Hélia H.
Data:  2009-03-01
Ano:  2009
Palavras-chave:  Cell immobilization
Glucosyltransferase
Klebsiella sp
Palatinose
Response surface methodology
Resumo:  The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%) were evaluated and the yield of palatinose was approximately 62.5%.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100010
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822009000100010
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.40 n.1 2009
Direitos:  info:eu-repo/semantics/openAccess
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