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Provedor de dados:  BJM
País:  Brazil
Título:  Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize
Autores:  Aquino,Simone
Ribeiro,Deise Helena Baggio
Villavicencio,Anna Lucia Casañas Haasis
Data:  2005-12-01
Ano:  2005
Palavras-chave:  Gamma radiation
Aspergillus flavus
Fluorescent viability test
Water activity
Resumo:  One of the currently most important fungi in stored grains is Aspergillus flavus, which produce aflatoxins. This fungus can grow on diverse substrates and represents a serious public health and animal nutritional problem. Therefore, the study of techniques that can be applied to the control of aflatoxins is of great importance. The objective of the present study was to determine the effects of gamma radiation on the growth of Aspergillus flavus Link and on degradation of aflatoxin B1 and B2 (AFB1 and AFB2) at a relative humidity of 97 99% and a water activity (Aw) of 0.88-0.94. Samples of corn grains were irradiated using a cobalt 60 source emitting gamma rays at doses of 2, 5 and 10 kGy. Irradiation was found to be effective in reducing the number colony-forming units of A. flavus, per gram, in the corn samples analyzed. In addition, the fluorescent viability test (fluorescein diacetate and ethidium bromide) revealed a decrease in the number of viable cells with increasing irradiation doses and three different fluorescence patterns. Furthermore, irradiation induced a partial reduction in AFB1 and AFB2 levels at the doses of 2 and 5 kGy, whereas complete degradation of aflatoxins was observed in the assay employing 10 kGy.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822005000400009
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.36 n.4 2005
Direitos:  info:eu-repo/semantics/openAccess

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