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Provedor de dados:  BJM
País:  Brazil
Título:  Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
Autores:  Fonseca,María Isabel
Tejerina,Marcos Raúl
Sawostjanik-Afanasiuk,Silvana Soledad
Giorgio,Ernesto Martin
Barchuk,Mónica Lucrecia
Zapata,Pedro Darío
Villalba,Laura Lidia
Data:  2016-06-01
Ano:  2016
Palavras-chave:  White rot fungi
Manganese peroxidase
Cu2+ response
Resumo:  Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2016.01.002
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.47 n.2 2016
Direitos:  info:eu-repo/semantics/openAccess

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