| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
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| Autores: |
González,Teresa
Licursi,María
Bonzo,Estela
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| Data: |
2007-03-01
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| Ano: |
2007
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| Palavras-chave: |
Bovine leukemia
ELISA evaluation
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| Resumo: |
Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100001
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822007000100001
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.38 n.1 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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