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Provedor de dados:  BJPS
País:  Brazil
Título:  Development of a generic method for the determination of proton-pump inhibitors by capillary zoneelectrophoresis
Autores:  Papp,Lajos Attila
Gyéresi,Árpád
Hancu,Gabriel
Mircia,Eleonora
Kelemen,Hajnal
Data:  2019-01-01
Ano:  2019
Palavras-chave:  Capillary electrophoresis
Proton-pump inhibitors
Experimental design
Resumo:  A generic capillary zone electrophoresis method was developed for the analysis of four proton pump inhibitors: omeprazole, pantoprazole, lansoprazole and rabeprazole. During preliminary analysis screening of phosphate buffers at different pH levels was performed, in order to determine the optimum pH domain suitable for the simultaneous determination of all studied compounds. A face centered central composite design was employed for the optimization of separation conditions. The effect of buffer concentration, pH and applied voltage was studied; resolution between peaks and migration time of the last compound were considered as responses. Other factors as system temperature, injection parameters, capillary length, were held constant during the optimization process. The optimized conditions consisted of 40mM phosphate background electrolyte at pH 5.0, +25 kV applied voltage and 20 °C temperature. The migration order of the analytes was as follows: rabeprazole, omeprazole, lansoprazole and pantoprazole. Full resolution of all analytes was achieved within 9 minutes. The method was validated and proved to be suitable in terms of repeatability, sensitivity, linearity, accuracy and robustness. Determinations from commercially available pharmaceutical formulation were performed for omeprazole; good reproducibility and recovery were obtained.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100534
Editor:  Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação:  10.1590/s2175-97902019000117786
Formato:  text/html
Fonte:  Brazilian Journal of Pharmaceutical Sciences v.55 2019
Direitos:  info:eu-repo/semantics/openAccess
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