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Provedor de dados:  Scientia Agricola
País:  Brazil
Título:  Marker-assisted selection of maintainer lines within an onion tropical population
Autores:  Santos,Carlos Antonio Fernandes
Leite,Daniela Lopes
Oliveira,Valter Rodrigues
Rodrigues,Marciene Amorim
Data:  2010-04-01
Ano:  2010
Palavras-chave:  Allium cepa
CMS
'Alfa São Francisco'
Tropical hybrid
Resumo:  The traditional onion (Allium cepa) hybrid production requires the development of maintainer and male sterile lines and also a pollinator line with good specific combination ability. We report the identification of maintainer and male-sterile onion lines within the Brazilian 'Baia Periforme' derived population, 'Alfa São Francisco', associating random field pairing of male-fertile plants with selected male-sterile plants and PCR-based marker system monitoring S, T and N-cytoplasms. Male-sterile plants produced flowers with light green anthers which were easily detected in the field. A frequency of 2.0% of male-sterile plants was estimated in the 'Alfa São Francisco' sampled population. Male-sterile plants produced the 5'cob-marker 180-bp and the orfA501-marker 473-bp fragments, suggesting the T-cytoplasm type, while the maintainer line produced only the 5'cob-marker 180-bp. These identified lines will be important to develop tropical onion hybrids well-adapted to Brazilian low latitudes and to future comparative studies with other onion cytoplasmic genic male sterility systems.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000200015
Editor:  São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação:  10.1590/S0103-90162010000200015
Formato:  text/html
Fonte:  Scientia Agricola v.67 n.2 2010
Direitos:  info:eu-repo/semantics/openAccess
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