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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration
Autores:  Rocha,Paulo Sérgio Gomes da
Oliveira,Roberto Pedroso de
Scivittaro,Walkyria Bueno
Data:  2013-07-01
Ano:  2013
Palavras-chave:  Saccharum officinarum
Light source
Tissue culture
White fluorescent lamp
Resumo:  The aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light sources and four medium sucrose concentrations) with six replications. Sugarcane bud growth was satisfactory under the three LED types studied. The presence of sucrose in growth media was essential for bud multiplication and rooting. Nevertheless, each light source requires the respective medium sucrose concentration adjustment for best results. Red LEDs provided a significantly high multiplication rate (although not the highest) with 8.5 buds per sub-culture and 34.9g L-1 of sucrose; also, the highest bud length (33.3mm) and the best plantlet acclimatization. Therefore, LED sources can advantageously substitute fluorescent lamps in laboratories of sugarcane micropropagation.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84782013000700005
Formato:  text/html
Fonte:  Ciência Rural v.43 n.7 2013
Direitos:  info:eu-repo/semantics/openAccess

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