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Provedor de dados:  Electron. J. Biotechnol.
País:  Chile
Título:  Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
Autores:  Tang,Yue Yi
Wang,Chuan Tang
Yang,Guan Pin
Gao,Hua Yuan
Wang,Xiu Zhen
Chi,Xiao Yuan
Xu,Ya Long
Chen,Dian Xu
Data:  2011-09-01
Ano:  2011
Palavras-chave:  Chilling response
Real-time quantitative PCR
Suppression subtractive hybridization
Resumo:  To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
Tipo:  Journal article
Idioma:  Inglês
Editor:  Pontificia Universidad Católica de Valparaíso
Formato:  text/html
Fonte:  Electronic Journal of Biotechnology v.14 n.5 2011

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