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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Autores:  Maia,Thais Augusta
Vilaça,Sibelle Torres
Silva,Luciana Resende da
Santos,Fabricio Rodrigues
Dantas,Gisele Pires de Mendonça
Data:  2017-12-01
Ano:  2017
Palavras-chave:  DNA
Noninvasive samples
Genetic diversity
Endangered birds
Mergus
Resumo:  Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/1678-4685-gmb-2016-0297
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.40 n.4 2017
Direitos:  info:eu-repo/semantics/openAccess
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