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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH
Autores:  Fernandes,Thiago
Rego,Letícia do Nascimento Andrade de Almeida
Yuyama,Priscila Mary
Vanzela,André Luís Laforga
Data:  2009-01-01
Ano:  2009
Palavras-chave:  Cestrum
Chromosome banding
Physical maps
45S and 5S rDNA
Resumo:  The karyotypes of four South American species of Cestrum (C. capsulare, C. corymbosum, C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA+ bands and 45S rDNA were located predominantly in terminal regions. The C-CMA+/DAPI+ bands appeared in interstitial and terminal regions, and the C-DAPI+ bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species except C. capsulare, where they were found in the paracentromeric region of the long arm of pair 4. The differences in band patterns among the species studied here, along with data from other nine species reported in the literature, suggest that the bands are dispersed in an equilocal and non-equilocal manner and that structural rearrangements can be responsible for internal karyotype diversification. However, it is important to point out that the structural changes involving repetitive segments did not culminate in substantial changes in the general karyotype structure concerning chromosome size and morphology.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572009000200019
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.32 n.2 2009
Direitos:  info:eu-repo/semantics/openAccess

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