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Provedor de dados:  J. Venom. Anim. Toxins incl. Trop. Dis.
País:  Brazil
Título:  Insects as biological models to assay spider and scorpion venom toxicity
Autores:  Manzoli-Palma,M. F.
Gobbi,N.
Palma,M. S.
Data:  2003-01-01
Ano:  2003
Palavras-chave:  Bioassay
Venom
Insect model
Toxicity
Apis mellifera
Resumo:  This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N. cruentata venom caused only paralysis at the doses assayed. A comparison between the insect test species described above revealed that A. mellifera was highly sensitive to all venoms tested; even a tiny amount of N. cruentata non-lethal venom caused a change in the walking pattern leading to transient paralysis. D. saccharalis larvae were very resistant to all four venoms.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200004
Editor:  Centro de Estudos de Venenos e Animais Peçonhentos
Relação:  10.1590/S1678-91992003000200004
Formato:  text/html
Fonte:  Journal of Venomous Animals and Toxins including Tropical Diseases v.9 n.2 2003
Direitos:  info:eu-repo/semantics/openAccess
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