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Provedor de dados:  PAB
País:  Brazil
Título:  Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain
Autores:  Silva,André Ribeiro da
Zanin,Lívia Montanheiro Médici
Ishikawa,Angélica Tieme
Yamashita,Cassia Reika Takabayashi
Fracalossi,Felipe Pedote
Amorim,Thaís Marques
Itano,Eiko Nakagawa
Hirooka,Elisa Yoko
Data:  2018-03-01
Ano:  2018
Palavras-chave:  Aspergillus
Aflatoxin B1
Blanched peanuts
Resumo:  Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively, were also recorded. The ic-Elisa showed a good reproducibility with a high-intraday precision, with 1.87% coefficient of variation (CV), and interday precision with 6.75% CV. The moisture content ranged from 4.0 to 7.2% (mean of 5.8%), and the water activity from 0.4848 to 0.6997 (mean of 0.5990) for the tested samples. Aflatoxin B1 was present in concentrations ranging from 1.13 to 29.2 μg kg-1, with only two samples (3.3%) exceeding the maximum allowed limit of 20 μg kg-1. The ic-ELISA developed here is an accessible tool for the rapid monitoring of aflatoxin contamination in the peanut production chain.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira
Relação:  10.1590/s0100-204x2018000300011
Formato:  text/html
Fonte:  Pesquisa Agropecuária Brasileira v.53 n.3 2018
Direitos:  info:eu-repo/semantics/openAccess

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