Consulta: creatorFacets:"ÁVILA,ANTÔNIO C. DE"
Registros recuperados: 3
Data/hora: 09/06/2024 15:31:09

Analysis of the nucleotide sequence of the coat protein and 3'-untranslated region of two Brazilian Potato virus Y isolates
Provedor de dados: 102
Autores: INOUE-NAGATA,ALICE K.; FONSECA,M. ESTHER N.; LOBO,TATIANA O.T.A.; ÁVILA,ANTÔNIO C. DE; MONTE,DAMARES C.
Two Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA corresponding to the 3' terminal region of the viral genome. The sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (NIb) gene, 804 nt of the coat protein (CP) gene, and 328 nt (PVY-OBR) or 326 nt (PVY-NBR) of the 3'-untranslated region (UTR). Translation of the sequence resulted in one single open reading frame with part of the NIb and a CP of 267 amino acids. The two isolates shared 95.1% similarity in the CP amino acid sequence. The CP and the 3'-UTR sequence of the Brazilian isolates were compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PVY; Potyvirus; Phylogenetic analysis; Taxonomy; Coat protein.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000100008

RT-PCR and Dot Blot hybridization methods for a universal detection of tospoviruses
Provedor de dados: 102
Autores: EIRAS,MARCELO; RESENDE,RENATO O.; MISSIAGGIA,ALEXANDRE A.; ÁVILA,ANTÔNIO C. DE.
Transcriptase reverse - polymerase chain reaction (RT-PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Zucchini lethal chlorosis virus, Iris yellow spot virus. Primers for PCR amplification were designed to match conserved regions of the tospovirus genome. RT-PCR using distinct primer combinations was unable to simultaneously amplify all tospovirus species and consistently failed to detect ZLCV and IYSV in total RNA extracts. However, all tospovirus species were detected by RT-PCR when viral RNA was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Plant viruses; Bunyaviridae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200009

Distribuição de geminivírus nas culturas do tomate e pimentão em doze municípios do Submédio do Vale São Francisco
Provedor de dados: 102
Autores: LIMA,MIRTES F.; BEZERRA,ISABEL C.; RIBEIRO,SIMONE G.; ÁVILA,ANTÔNIO C. DE.
Em 1996 e 1997, observaram-se sintomas de viroses causadas por geminivírus transmitidos por mosca branca em plantas de tomateiro (Lycopersicon esculentum) e pimentão (Capsicum annuum) no Submédio do Vale São Francisco, situado nos Estados de Pernambuco e Bahia. De outubro de 1996 a dezembro de 1998, foram coletadas 1.368 amostras foliares de tomateiro e 194 de pimentão, exibindo sintomas similares àqueles causados por geminivírus, em 104 e 16 plantios, respectivamente, de 12 municípios dessa região e em dois municípios vizinhos. A incidência de geminiviroses nas áreas amostradas foi estimada entre 5 e100% para tomate e entre 10 e 20% para pimentão. A detecção de geminivírus nas amostras foi feita por "dot blot" ou "squash blot" utilizando-se sondas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Capsicum annuum; Epidemiologia; Begomovirus.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000100014