Consulta: creatorFacets:"松井, 基純"
Registros recuperados: 58
Data/hora: 06/06/2024 01:28:50

インスリン感受性試験のための至適インスリン投与濃度の検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成20年度 1.基盤研究部門
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2511

育成牛の繁殖管理
Provedor de dados: 19
Autores: 松井, 基純.
DAIRYMAN秋季臨時増刊号「徹底・後継牛づくり」第1部 飼養管理編 2章 育成牛の管理 3 育成牛の繁殖管理
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3085

Relationship between the degree of insulin resistance during late gestation and postpartum performance in dairy cows and factors that affect growth and metabolic status of their calves
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; MUNAKATA, Megumi; SHIMIZU, Takashi; MIYAMOTO, Akio; KIDA, Katsuya; MATSUI, Motozumi; 川島, 千帆; 清水, 隆; 宮本, 明夫; 木田, 克弥; 松井, 基純.
This study aimed to investigate the effects of insulin resistance (IR) during the close-up dry period on the metabolic status and performance of dairy cows as well as to determine the effects on body weight (BW) and metabolic status of their calves. An insulin tolerance test (ITT) was conducted by administering 0.05 IU/kg BW of insulin to 34 multiparous Holstein cows at 3 weeks prepartum. Blood samples were collected at 0, 30, 45 and 60 min after insulin injection, and cows were divided into two groups based on the time required for glucose to reach the minimum levels [non-IR (NIR), 45 min (n=28); and IR, 60 min (n=6)]. Blood or milk sampling and body condition score (BCS) estimation were performed twice weekly during the experimental period. Blood samples...
Palavras-chave: Dairy cow; Dry period; Insulin tolerance test; Metabolic status; Postpartum performance.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4363

Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows
Provedor de dados: 19
Autores: Kawashima, Chiho; Fukihara, Saori; Maeda, Mayumi; Kaneko, Etsushi; Montoya, Carlos Amaya; Matsui, Motozumi; Shimizu, Takashi; Matsunaga, Nobuyoshi; Kida, Katsuya; Miyake, Yoichi; Schams, Dieter; Miyamoto, Akio; 川島, 千帆; 松井, 基純; 清水, 隆; 木田, 克弥; 三宅, 陽一; 宮本, 明夫.
Recent studies suggest that IGF-I is a crucial regulatory factor in follicular growth during early post-partum period. The aim of the present study was to determine in detail the changing profiles of metabolic and reproductive hormones in relation to ovulation of the dominant follicle (DF) of the first follicular wave post-partum in high-producing dairy cows. Plasma concentrations of related hormones in 22 multiparous Holstein cows were measured from 4 weeks pre-partum to 3 weeks post-partum, and the development of DF was observed with colour Doppler ultrasound. Thirteen cows showed ovulation by 15.2 days post-partum. Anovulatory cows showed higher GH and lower IGF-I levels than those in ovulatory cows during the peri-partum period. Each DF developed...
Palavras-chave: GROWTH-FACTOR-I; COMPETITIVE ENZYME-IMMUNOASSAY; ENERGY-BALANCE; OVARIAN ACTIVITY; STIMULATING-HORMONE; ESTRADIOL PRODUCTION; BODY CONDITION; MESSENGER-RNA; HOLSTEIN COWS; IGF-I.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1658

ペルシュロン種繁殖雌馬にみられた遠隔転移を伴う腎細胞癌の1症例
Provedor de dados: 19
Autores: 久保田, 直樹; 村上, 智亮; 安田, 峰; 松井, 基純; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽.
19歳齢のペルシュロン種繁殖雌馬に血尿が認められた。血尿は止血剤および抗生剤投与によっては改善され なかった。患畜は次第に元気食欲の低下、削痩、貧血を呈し、高度蛋白尿および胸前部冷性浮腫が発現した。 直腸検査により人頭大に腫大した左腎を触知し、腎臓腫瘍を疑った。病理解剖の結果、左腎は27X18Xl0cmに 腫大し、血液に富む巨大な腫瘤形成(13X8X8cm)を認めた。左腎門リンパ節は腫大し、また右腎臓、左右 副腎、肝臓および肺では大小の白色腫瘤を認めた。病理組織学的検査によりこれらの腫瘍はいずれも浸潤性に 増殖する上皮性腫瘍組織であったことから、本症例は腎細胞癌が他の複数の臓器に転移したものと考察した。
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2935

LHサージ直前の排卵前卵胞の吸引とその後の継続的な卵胞吸引による黄体不在の延長を利用した卵胞嚢腫作出モデルの開発に関する研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2559

Relationships Between the First Ovulation Postpartum and Polymorphism in Genes Relating to Function of Immunity, Metabolism and Reproduction in High-producing Dairy Cows
Provedor de dados: 19
Autores: SHIRASUNA, Koumei; KAWASHIMA, Chiho; MURAYAMA, Chiaki; AOKI, Yuka; MASUDA, Yutaka; KIDA, Katsuya; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 川島, 千帆; 増田, 豊; 木田, 克弥; 松井, 基純; 清水, 隆; 宮本, 明夫.
The decrease in fertility and conception rates of high-producing dairy cows is one of the major negative impacts for today's producers. The recovery of ovarian activity postpartum is affected by the status of immunity, metabolism and reproduction and plays a critical role in subsequent fertility after parturition in the cow. In the present study we investigated the relationships between polymorphisms in genes relating to the above functions and the first postpartum ovulation as a marker of the recovery of ovarian function in the cow. In immune function related-factors, the occurrence of first postpartum ovulation within 3 weeks in the C/C genotypes of tumor necrosis factor α (TNFα) exon (55.4%) and the A/G genotypes of TNFα promoter (55.4%) was...
Palavras-chave: Cow; Ovulation; Polymorphism; Tumor necrosis factor α (TNFα).
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2990

乳牛の周産期におけるインスリン感受性およびインスリン分泌能と分娩後の繁殖性の関係
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2148

The Effects of Labial Stimulation after Artificial Insemination on Conception Rates in Holstein Heifers
Provedor de dados: 19
Autores: 小野, 守; 小関, 茂樹; 斉藤, 康倫; 泉, 徳和; 松井, 基純; 大澤, 健司; 三宅, 陽一; MATSUI, Motozumi.
人工授精 (AI) 後の陰唇刺激がホルスタイン種乳用牛の受胎率に及ぼす効果を評価するために, 未経産牛295頭を用い, AI直後に手指による陰唇刺激を15秒間行って対照群と比較した. その結果, 陰唇刺激群の受胎率は対照群よりも高かった (69.2%vs64.7%) が, 有意差はなかった. また, 対照群において7月から8月に受胎率が低下する傾向が認められたが, 陰唇刺激群では認められなかった. 両群の受胎成績のTemperature-Humidity Index (THI) 別の分析では, 対照群において, 暑熱ストレス条件下とされるTHIが72以上の場合の受胎率は, 72以下の場合よりも有意に低かった (P&lt;0.01) が, 陰唇刺激群ではそのような差異は認められなかった.
Palavras-chave: 受胎率; 乳用未経産牛; 陰唇刺激.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4179

外陰部刺激がホルスタイン種雌牛の子宮内圧に与える影響に関する研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2560

春期発動前の雌ウシにおけるキスペプチンの作用に関する検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 1.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2555

LHサージ直前の排卵前卵胞の吸引とその後の継続的な卵胞吸引による黄体不在の延長を利用した卵胞嚢腫作出モデルの開発、ならびに、作出したモデルにおける発育過程の嚢腫卵胞の研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成20年度 1.基盤研究部門
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2509

Ovulation of the preovulatory follicle originating from the first-wave dominant follicle leads to formation of an active corpus luteum
Provedor de dados: 19
Autores: MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純.
The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =...
Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147

Expression of mRNA for the Angiopoietin-Tie System in Granulosa Cells during Follicular Development in Cows
Provedor de dados: 19
Autores: HAYASHI, Ken-Go; BERISHA, Bajram; MATSUI, Motozumi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17β (E2) concentration in follicular fluid (FF) as follows: (1) E2&lt;0.5, (2) 0.5&lt;E2&lt;5, (3) 5&lt;E2&lt;20, (4) 20&lt;E2&lt;180 and (5)...
Palavras-chave: Follicular development; Angiopoietin-Tie system; Granulosa cell; Cow.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2975

人工授精後の黄体の形態および機能が受胎の成立に及ぼす影響
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2151

Changes in Plasma Metabolic Hormone Concentrations During the Ovarian Cycles of Japanese Black and Holstein Cattle
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; KIDA, Katsuya; HAYASHI, Ken-Go; AMAYA MONTOYA, Carlos; KANEKO, Etsushi; MATSUNAGA, Nobuyoshi; SHIMIZU, Takashi; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; SCHAMS, Dieter; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 清水, 隆; 松井, 基純; 三宅, 陽一; 宮本, 明夫.
The aim of the present study was to investigate the changing profiles of plasma metabolic hormones during the ovarian cycles of beef and dairy cattle. We used 16 non-pregnant, non-lactating Japanese Black beef cattle (6 heifers and 10 cows; parity=2.3 ± 0.8) and 12 multiparous Holstein dairy cows (parity=3.0 ± 0.3). Blood samples for hormonal analysis (growth hormone, GH; insulin-like growth factor-I, IGF-1; insulin; and progesterone, P4) were obtained twice weekly for 40 days before artificial insemination for Japanese Black cattle and from 50 to 100 days postpartum for Holstein cows. Luteal phases were considered normal if the P4 concentrations for at least 3 time points over the course of 7 days remained above 1 ng/ml and at least 2 of the time points...
Palavras-chave: Cattle; Insulin-like growth factor-I; Ovarian cycle; Progesterone.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2984

Slow recovery of blood glucose in the insulin tolerance test during the prepartum transition period negatively impacts the nutritional status and reproductive performance postpartum of dairy cows.
Provedor de dados: 19
Autores: LEE, Hsu-Hsun; KIDA, Katsuya; MIURA, Ryotaro; INOKUMA, Hisashi; MIYAMOTO, Akio; KAWASHIMA, Chiho; HANEDA, Shingo; MIYAKE, Yoh-Ichi; MATSUI, Motozumi; 猪熊, 壽; 宮本, 明夫; 川島, 千帆; 羽田, 真悟; 松井, 基純.
In peripartum dairy cows, insulin resistance (IR) increases to adjust the direction of energy to lactation after calving. To investigate the effect of prepartum IR on postpartum reproductive performance, the insulin tolerance test (ITT) was applied to 15 cows at 3 weeks (Pre21) and 10 days (Pre10) before the predicted calving date. Blood glucose area under the curve (AUCglu) within 120 min after administration of 0.05 IU/kg-BW insulin was calculated. The occurrence of first ovulation, days to first artificial insemination (AI) and first AI conception rate were recorded. Nutritional status postpartum was evaluated by blood chemical analysis. Based on AUCglu changes from Pre21 to Pre10, cows were classified into either the AUC-up group (AUCglu increase, n=5)...
Palavras-chave: Dairy cows; Glucose metabolism; Insulin tolerance test; Reproductive performance; Transition period.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4129

ウシ優性卵胞と閉鎖卵胞の卵胞液中におけるN-アセチルグルコサミニルトランスフェラーゼ I 活性の解析
Provedor de dados: 19
Autores: Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡.
The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose&gt; α1-3α1-6 mannotriose&gt;α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large...
Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185

第37回アメリカ生殖学会および第15回国際家畜繁殖学会での学会発表
Provedor de dados: 19
Autores: 松井, 基純.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/661

Relationship between Peripartal Plasma Oxytocin and Prostaglandin F2α Metabolite and Placental Expulsion Time in Heavy Draft Mares
Provedor de dados: 19
Autores: ISHII, Mitsuo; KOBAYASHI, Syuichi; ACOSTA, Tomas J.; MIKI, Wataru; YAMANOI, Takahiro; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 石井, 三都夫; 松井, 基純; 三宅, 陽一; 宮本, 明夫.
The aim of this study was to clarify the relationship between circulating oxytocin (OT) and PGF2α metabolite (PGFM) in mares at the third stage of labor and placental expulsion time in order to investigate a cause of retained placenta of which the incidence increase in a heavy draft mare. Blood was sampled every 5 min from foaling to expulsion of the placenta in 18 heavy draft mares to evaluate circulating OT and PGFM. The relationships between OT and PGFM concentration and recorded placental expulsion times were investigated. The results were as follows (1) The highest level of OT concentration was observed close to foaling in 15 mares. (2) The OT concentrations close to foaling were variable with a large difference from the lowest concentration, 22.1...
Palavras-chave: Heavy draft mare; Oxytocin; 15-keto-dihydro prostaglandin F2α (PGFM); Placental expulsion.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3089

授精後早期の黄体機能、生殖内分泌および栄養状態からみた受胎成立要件に関する検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 1.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2554

分娩後の卵巣機能および発情行動の回帰に関する研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2149

乳牛の第一卵胞波における優勢卵胞の機能を制御する要因に関する検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成20年度 1.基盤研究部門
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2510

現代の高泌乳牛の健康医科学と新しい生殖技術の構築
Provedor de dados: 19
Autores: 宮本, 明夫; 手塚, 雅文; 松井, 基純; 木田, 克弥; 左, 久; 清水, 隆.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3511

ウシ第一卵胞波における過剰排卵処置の有効性
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2150

Influence of hepatic load from far-off dry period to early postpartum period on the first postpartum ovulation and accompanying subsequent fertility in dairy cows
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; ITO, Nozomi; NAGASHIMA, Shuntarou; MATSUI, Motozumi; SAWADA, Kumiko; SCHWEIGERT, Florian J.; MIYAMOTO, Akio; KIDA, Katsuya; 川島, 千帆; 松井, 基純; 宮本, 明夫; 木田, 克弥.
The aim of the present study was to investigate nutritional and metabolic parameters during the dry and early postpartum periods of ovulatory and anovulatory cows, as well as their postpartum reproductive performance. Blood samples from 20 multiparous Holstein cows were collected once a week from the far-off dry period to 3 weeks postpartum. Early postpartum (0–3 weeks) ovulation was confirmed using plasma progesterone concentration profiles, and cows were considered ovulatory if they had resumed luteal activity by this point (n = 9), whereas cows that had not were considered anovulatory (n = 11). Data from the ovulatory and anovulatory cows were analyzed separately for the far-off dry period (7–4 weeks prepartum), the close-up dry period (3–1 weeks...
Palavras-chave: Dairy cow; First ovulation; Hepatic enzyme; Liver function; Reproductive performance.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4308

カナダ・ゲルフ大学における生産獣医療に関する卒後教育プログラム
Provedor de dados: 19
Autores: 松井, 基純.
海外情報
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3084

初診時ミイラ胎子を疑った成牛型牛白血病の乳牛の1症例
Provedor de dados: 19
Autores: 出口, 祐一郎; 秋場, 由美; 松井, 基純; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 三宅, 陽一; 猪熊, 壽; 石井, 三都夫.
5歳7ヶ月齢のホルスタイン種雌乳牛で初診時、直腸検査にて子宮内にミイラ胎子様のものが触知された。プロスタグランジンF2α 製剤には反応せず、経過を観察していたところ、第43病日にエコー検査により子宮内腫瘤を認め、また第81病日に起立不能となった。病理解剖の結果、両子宮壁に直径3-15cm大の硬結感を有する腫瘤が多数みられ、また腹腔内および胸腔にも腫瘤が認められた。BLV抗体は陽性であり、最終的に成牛型牛白血病と診断された。本症例では体表リンパ節および内腸骨リンパ節の腫脹が認められず、末梢血中リンパ球の増加等もみられなかったことから、生前の牛白血病の診断が困難であった。
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2778

In Vivo Evidence that Local Cortisol Production Increases in the Preovulatory Follicle of the Cow
Provedor de dados: 19
Autores: ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫.
The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and...
Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980

Prostaglandin F2α Differentially Affects mRNA Expression Relating to Angiogenesis，Vasoactivation and Prostaglandins in the Early and Mid Corpus Luteum in the Cow
Provedor de dados: 19
Autores: SHIRASUNA, Koumei; SASAHARA, Kiemi; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 松井, 基純; 清水, 隆; 宮本, 明夫.
Administration of prostaglandin (PG) F2α in cattle during the mid-luteal phase (Days 8-12 of the estrous cycle) drastically reduces the plasma progesterone concentrations and the volume of the corpus luteum (CL). However, PGF2α does not induce luteolysis during the early luteal phase (up to Day 5 of the estrous cycle). To characterize the possible distinct difference in acute response to a luteolytic dose of PGF2α administration, we determined various mRNA expressions in the early and mid CL relating to angiogenesis, vasoactivation and PG-related factors at 30 min after PGF2α injection in cyclic cows. The experiments were conducted on Day 4 (early CL) and Days 10-12 (mid CL). Cows were either injected with 500 μg PGF2α analogue or saline as the control...
Palavras-chave: Corpus luteum; Cow; Luteolysis; Prostaglandin F2α.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2812

An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro
Provedor de dados: 19
Autores: LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946

卵巣割拠雌牛へのテストステ口ン投与による発情発見牛作成の試み
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成20年度 1.基盤研究部門
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2512

An attempt to induce antibody production for immunocontraception in the Hokkaido sika deer (Cervus nippon yesoensis) by immunization with a porcine zona pellucida synthetic peptide
Provedor de dados: 19
Autores: Kobayashi, Kohei; Asano, Makoto; Yanagawa, Yojiro; Haneda, Singo; Matsui, Motozumi; 羽田, 真悟; 松井, 基純.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4130

LHサージ直前の排卵前卵胞の吸引とその後の継続的な卵胞吸引による黄体不在の延長を利用した卵胞嚢腫作出モデルの開発
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2147

妊娠牛、非妊娠牛および未授精牛における末梢血白血球由来ISG15、Mx2発現量の比較と黄体機能との関連
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成20年度 1.基盤研究部門
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2508

帯広畜産大学第7回公開総合臨床学実習における Hands-on laboratory の概要
Provedor de dados: 19
Autores: 佐々木, 直樹; 山田, 一考; 伊藤, めぐみ; 柳川, 将志; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 堀内, 雅之; 南保, 泰雄; 猪熊, 壽.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4506

乳牛のインスリン抵抗性を指標とした分娩前栄養代謝状態の変化が分娩後の繁殖機能へ及ぼす影響
Provedor de dados: 19
Autores: 松井, 基純.
平成21年度 Ⅰ.基盤研究部門
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3353

乾乳期の栄養代謝内分泌状況が、分娩後の栄養状態および卵巣機能に及ぼす影響に関する検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2558

妊娠牛、非妊娠牛および未授精牛での末梢血白血球由来ISG15、GM-CSF発現量と受胎性との関連
Provedor de dados: 19
Autores: 松井, 基純.
平成21年度 Ⅰ.基盤研究部門
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3352

春機発動前の雌ウシにおけるキスペプシンの作用の検討
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2141

Cryopreservation of Canine Ovaries by Vitrification
Provedor de dados: 19
Autores: ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安.
The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23...
Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023

Effect of intraluteal injection of endothelin type A receptor antagonist on PGF2alpha-induced luteolysis in the cow.
Provedor de dados: 19
Autores: Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫.
Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3...
Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922

Relative Changes in mRNA Expression of Angiopoietins and Receptors Tie in Bovine Corpus Luteum during Estrous Cycle and Prostaglandin F2α-induced Luteolysis: A Possible Mechanism for the Initiation of Luteal Regression
Provedor de dados: 19
Autores: TANAKA, Jun; ACOSTA, Tomas J.; BERISHA, Bajram; TETSUKA, Masa; MATSUI, Motozumi; KOBAYASHI, Shuichi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Local angiogenesis and angiolysis in the corpus luteum (CL) relate to the luteal function. Recent studies indicate that angiopoietins (ANPT) and their receptors Tie regulate remodeling of microvasculature. We therefore examined 1) the relative changes in the expression of mRNA for ANPT-1, ANPT-2, Tie1 and Tie2 in bovine CL by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) during the estrous cycle and prostaglandin F2α (PGF2α)-induced luteolysis, and 2) the effect of ANPT-2 on progesterone (P4) release from CL at the late stage of the estrous cycle by an in vitro microdialysis system (MDS). The CLs were classified into 4 stages (early: Day 2-5, n=7, mid: Day 8-12, n=15, late: Day 15-17, n=9, regressing: Day &gt;18, n=19). The...
Palavras-chave: Angiopoietin; Angiolysis; Luteolysis; Corpus luteum; Cow.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2977

ウシ第一卵胞波における過剰排卵処置の有効性に関する研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 1.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2556

Relationship between the First Ovulation within Three Weeks Postpartum and Subsequent Ovarian Cycles and Fertility in High Producing Dairy Cows
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; KANEKO, Etsushi; AMAYA MONTOYA, Carlos; MATSUI, Motozumi; YAMAGISHI, Norio; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; KIDA, Katsuya; MIYAKE, Yoichi; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 石井, 三都夫; 木田, 克弥; 三宅, 陽一; 宮本, 明夫.
The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous...
Palavras-chave: Dairy cow; First ovulation; Postpartum; Resumption of ovarian cycle.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/916

A Potential Use of Color Ultrasound as a Tool for Reproductive Management: New Observations Using Color Ultrasound Scanning that were not Possible with Imaging Only in Black and White
Provedor de dados: 19
Autores: MIYAMOTO, Akio; SHIRASUNA, Koumei; HAYASHI, Ken-Go; KAMADA, Daichi; KAWASHIMA, Chiho; KANEKO, Etsushi; ACOSTA, Tomas J.; MATSUI, Motozumi; 宮本, 明夫; 白砂, 孔明; 川島, 千帆; 松井, 基純.
Ultrasonography (US) has been applied to the ovary and the uterus of domestic animals from the late 1980s, and established in 1990s as a practical tool for animal production. US made it possible to detect pregnancy at a very early stage and, most importantly, to observe the real-time dynamics of follicular development and hence the discovery of follicular waves. This has greatly contributed to our understanding of ovarian physiology and helped us to develop several "pin-point" protocols for hormonal treatment. While US may not seem to fit preconceived ideas of a "green" technology, it does not contravene environmental priorities, and it is non-invasive ("ethical") and non-hormonal ("clean"). Using the US technology that is now commercially available at a...
Palavras-chave: Color Doppler ultrasound; Blood flow; Ovary; Reproduction; Cow.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2983

Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum
Provedor de dados: 19
Autores: TAKAHASHI, Hiroto; HANEDA, Shingo; KAYANO, Mitsunori; MATSUI, Motozumi; 羽田, 真悟; 茅野, 光範; 松井, 基純.
Because the establishment of pregnancy begins at the uterine horn ipsilateral to the corpus luteum (ipsi-horn) in cattle, levels of progesterone (P4) and receptor expression in the endometrial tissue, which regulate the intrauterine environment for embryo development, may differ between the ipsi-horn and the uterine horn contralateral to corpus luteum (contra-horn). The aim of the present study was to determine the endometrial tissue P4 concentrations and nuclear progesterone receptor (PGR), progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 mRNA expressions in the cranial and middle parts of the uterine horns during the luteal phase. The results showed higher endometrial tissue P4 concentrations in the cranial part of the ipsi-horn than in...
Palavras-chave: Bovine; Progesterone; Receptor; Uterus.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4364

Effect of Suckling on the Reproductive Performance and Metabolic Status of Obese Japanese Black Cattle during the Early Postpartum Period
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; KIDA, Katsuya; MATSUHASHI, Miki; MATSUI, Motozumi; SHIMIZU, Takashi; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 松井, 基純; 清水, 隆; 石井, 三都夫; 三宅, 陽一; 宮本, 明夫; 松長, 延吉.
The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the...
Palavras-chave: Japanese Black cattle; Metabolic status; Obesity; Reproductive performance; Suckling.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2991

LHサージ直前の排卵前卵胞の吸引により引き起こされる黄体不在下での優勢卵胞の卵胞液の解析および卵胞発育の観察
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2140

Effect of the Dominant Follicle Aspiration before or after Luteinizing Hormone Surge on the Corpus Luteum Formation in the Cow
Provedor de dados: 19
Autores: HAYASHI, Ken-Go; MATSUI, Motozumi; ACOSTA, Tomas J.; KIDA, Katsuya; MIYAMOTO, Akio; 松井, 基純; 木田, 克弥; 宮本, 明夫.
Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α....
Palavras-chave: Corpus luteum formation; Cow; Follicle aspiration; LH surge.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2982

Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows
Provedor de dados: 19
Autores: ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: &gt; 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h...
Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979

分娩後におけるホルモン処置を用いた排卵誘起が、その後の卵巣機能および受胎率に及ぼす影響に関する研究
Provedor de dados: 19
Autores: 松井, 基純; Matsui, Motozumi.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2557

帯広畜産大学 第6回公開総合臨床学実習におけるナチュラル・ホースマンシップ実習ならびにHands-on臨床トレーニングの概要
Provedor de dados: 19
Autores: 佐々木, 直樹; 山田, 一孝; 柳川, 将志; 松井, 基純; 松本, 高太郎; 古岡, 秀文; 南保, 泰雄; 猪熊, 壽.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4210

産業動物獣医師就業研修報告 : 帯広畜産大学における公開総合臨床学実習の概要
Provedor de dados: 19
Autores: 石井, 三都夫; 佐々木, 直樹　; 山田, 一孝; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 古岡, 秀文; 古林, 与志安; 猪熊, 壽; 三宅, 陽一.
http://nichiju.lin.gr.jp/mag/06303/index.html
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2937

A cooperative action of endothelin-1 with prostaglandin F-2 alpha on luteal function in the cow
Provedor de dados: 19
Autores: Shirasuna, Koumei; Watanabe, S.; Oki, N.; Wijayagunawardane, M. P. B.; Matsui, Motozumi; Ohtani, M.; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫.
Prostaglandin F-2 alpha (PGF(2 alpha)) is the primary luteolysin in the cow, and luteal endothelin-1 (ET-1) interacts with PGF(2 alpha) during the process of luteolysis. In contrast, a developing corpus luteum (CL) is refractory to exogenous administration of PGF(2 alpha). Thus, the present study was aimed to investigate the functional relationship between ET-1 and PGF(2 alpha) in the mid-CL (PGF(2 alpha)-sensitive) and early-CL (PGF(2 alpha)-refractory). In the mid-CL model, cows (n = 6/treatment) were assigned to receive one of five types of treatments on day 10 of the estrous cycle: (1) an injection of saline; control, (2) a 500 jig of PGF2. analogue (sufficient dose to induce luteolytis); full-PG, (3) an intraluteal injection of 0.25 mg ET-1; ET-1, (4)...
Palavras-chave: Endothelin-1; Prostaglandin F-2 alpha; Luteolysis; Corpus luteum; Cow.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/825

重度雄性化を伴う褐毛和種フリーマーチン肥育牛にみられた腎後性腎不全の1例
Provedor de dados: 19
Autores: 吉本, 薫; 海老名, 千尋; 高橋, 英二; 古岡, 秀文; 松本, 高太郎; 松井, 基純; 三宅, 陽一; 猪熊, 壽; YOSHIMOTO, Kaoru; EBINA, Chihiro; TAKAHASHI, Eiji; FURUOKA, Hidefumi; MATSUMOTO, Kotaro; MATSUI, Motozumi; MIYAKE, Yo-ichi; INOKUMA, Hisashi.
排尿異常を認める21カ月齢の褐毛和種フリーマーチン肥育牛が，臨床的に水腎症および腎不全と診断された. 病理学的検査において，泌尿生殖器の重度雄性化，腎膿瘍および尿路結石が明らかとなった. 本症例は異常な尿道形態から尿路感染症と尿石症を継発し，最終的に腎後性腎不全に陥ったまれなフリーマーチン症例である. A Japanese Brown freemartin (21 months old) was clinically diagnosed with hydroneprosis and renal failure. A pathological examination revealed severe masculinization of the urogenital organs， pyonephritis and urolithiasis. These findings suggested that it was a rare case of a freemartin with a post-renal failure and morphological anomaly of the urinary tract，complicated by a bacterial infection and urolithiasis. 一一一Keywords :fr eemartin，po st-renal failure，se vere masculinization
Palavras-chave: フリーマーチン; 腎後性腎不全; 重度雄性化; Freemartin; Post-renal failure; Severe masculinization.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2944

Nutritional factors that regulate ovulation of the dominant follicle during the first follicular wave postpartum in high-producing dairy cows.
Provedor de dados: 19
Autores: KAWASHIMA, Chiho; MATSUI, Motozumi; SHIMIZU, Takashi; KIDA, Katsuya; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 清水, 隆; 木田, 克弥; 宮本, 明夫.
During recent decades, milk production per cow has increased drastically due to improved management, nutrition, and genetic selection; however, the reproductive performance of high-producing dairy cows has been declining. One of the factors responsible for this low reproductive performance is negative energy balance (NEB). NEB affects the onset of first ovulation in early postpartum cows. It is generally accepted that early first ovulation positively relates to the resumption of normal ovarian function, first service, and conception rate in dairy cows. Hence, delayed first ovulation has a negative impact on subsequent fertility. The metabolic condition of cows in NEB shifts to catabolic metabolism, which in turn causes increased plasma growth hormone and...
Palavras-chave: Β-Carotene; Dairy cow; First follicular wave postpartum; First ovulation; Metabolic hormone.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4041

帯広畜産大学 第3回公開総合臨床学実習(馬専攻・牛専攻)の概要
Provedor de dados: 19
Autores: 佐々木, 直樹; 山田, 一孝; 石井, 三都夫; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 古岡, 秀文; 古林, 与志安; 猪熊, 壽; SASAKI, Naoki; YAMADA, Kazutaka; ISHII, Mitsuo; Matsui, Motozumi; HANEDA, Shingo; Matsumoto, Kotaro; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4378