Consulta: creatorFacets:"Abdelnoor,Ricardo Vilela"
Registros recuperados: 7
Data/hora: 13/06/2024 02:58:34

Genetic relationships between Chinese, Japanese, and Brazilian soybean gene pools revealed by simple sequence repeat (SSR) markers
Provedor de dados: 74
Autores: Yamanaka,Naoki; Sato,Hiroyuki; Yang,Zhenyu; Xu,Dong He; Catelli,Lizandra Lucy; Binneck,Eliseu; Arias,Carlos Alberto Arrabal; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima.
An understanding of the relationship of geographically different soybean gene pools, based on selectively neutral DNA markers would be useful for the selection of divergent parental cultivars for use in breeding. We assessed the relationships of 194 Chinese, 59 Japanese, and 19 Brazilian soybean cultivars (n = 272) using 12 simple sequence repeat (SSR) markers. Quantification Theory III and clustering analyses showed that the Chinese and Japanese cultivars were genetically quite distant to each other but not independent, while Brazilian cultivars were distantly related to the cultivars from the other two countries and formed a cluster that was distant from the other two gene pool clusters. Our results indicated that the Brazilian soybean gene pool is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA marker; Genetic relationship; Genetic resources; Glycine max.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100016

Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease
Provedor de dados: 74
Autores: Bücker Neto,Lauro; Oliveira,Rafael Rodrigues de; Wiebke-Strohm,Beatriz; Bencke,Marta; Weber,Ricardo Luís Mayer; Cabreira,Caroline; Abdelnoor,Ricardo Vilela; Marcelino,Francismar Correa; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal disease; HyPRP genes; Glycine max; Real time RT-qPCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200012

New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta
Provedor de dados: 74
Autores: Rincão,Michelle Pires; Carvalho,Mayra Costa da Cruz Gallo de; Nascimento,Leandro Costa; Lopes-Caitar,Valéria S.; Carvalho,Kenia de; Darben,Luana M.; Yokoyama,Alessandra; Carazzolle,Marcelo Falsarella; Abdelnoor,Ricardo Vilela; Marcelino-Guimarães,Francismar Correa.
Abstract Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi, presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were active in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian soybean rust; Multigenic families; Transposable elements.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400671

Caracterização molecular de populações do nematóide-de-cisto-da-soja com diferentes índices de parasitismo na cultivar Hartwig
Provedor de dados: 86
Autores: Abdelnoor,Ricardo Vilela; Dias,Waldir Pereira; Silva,João Flávio Veloso; Marin,Silvana Regina Rockenbach; Kiihl,Romeu Afonso de Souza.
Recentemente, foi descoberta uma raça do nematóide-de-cisto-da-soja (NCS; Heterodera glycines) que apresentou a capacidade de quebrar a resistência da cultivar Hartwig, até então considerada resistente a todas as raças conhecidas do nematóide. Essa população foi coletada no Município de Sorriso, Estado do Mato Grosso, e foi caracterizada como raça 4. Para verificar a diversidade genética entre esta e outras populações pertencentes às raças 4 e 9, foi feita uma caracterização molecular pela técnica de marcadores moleculares RAPD. Foram utilizadas nove populações do NCS, das quais quatro apresentavam a capacidade de parasitar 'Hartwig'. Foi verificado que as populações capazes de parasitar 'Hartwig' foram bastante diferentes das demais. Por meio de análise...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterodera glycines; Variação genética; Marcadores moleculares; Polimorfismo.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001000200017

Identification of quantitative trait loci for resistance against soybean sudden death syndrome caused by Fusarium tucumaniae
Provedor de dados: 86
Autores: Yamanaka,Naoki; Fuentes,Francisco Horacio; Gilli,Javier Ramon; Watanabe,Satoshi; Harada,Kyuya; Ban,Tomohiro; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima; Homma,Yoshihisa.
The objective of this work was to identify genomic regions that underlie resistance to Fusarium tucumaniae sp. nov., the causing agent of sudden death syndrome (SDS) in soybean in South America, using a population with a genetic background different from that previously reported for Fusarium virguliforme sp. nov. (F. solani f. sp. glycines), also responsible for SDS in soybean. Although major genes and quantitative trait loci (QTL) for SDS resistance have been identified, little is known about the same disease caused by Fusarium tucumaniae sp. nov., in South America. To identify genetic factors related to resistance to F. tucumaniae and DNA markers associated with them, a QTL analysis was performed using recombinant inbred lines. The map locations of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Disease index; Residual heterozygous line; SDS.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000900006

Enzimas marcadoras de indução de resistência diferencialmente reguladas em soja resistente e suscetível à ferrugem-asiática-da-soja
Provedor de dados: 86
Autores: Almeida,Hebréia Oliveira; Barbosa,Meire de Oliveira; Marques,Ana Ermelinda; Pereira,Tânus Henrique Abdalla; Magalhães Júnior,Marcos Jorge; Tessarollo,Nayara Gusmão; Games,Patrícia Dias; Barros,Everaldo Gonçalves de; Stolf-Moreira,Renata; Marcelino-Guimarães,Francismar Corrêa; Abdelnoor,Ricardo Vilela; Pereira,Paulo Roberto Gomes; Baracat-Pereira,Maria Cristina.
O objetivo deste trabalho foi avaliar, por meio de enzimas marcadoras, a indução de resistência à ferrugem-asiática-da-soja em genótipos de soja contrastantes quanto à suscetibilidade a Phakopsora pachyrhizi. Aproteína total e as atividades de cinco enzimas marcadoras da indução de resistência (lipoxigenases, peroxidases, fenilalanina amônia-liase, quitinases e β-1, 3-glucanases) foram avaliadas em extratos de folhas de plantas de soja dos genótipos Embrapa 48 (suscetível) e PI 561356 (resistente), submetidas à inoculação ou não com o patógeno. Foram observadas respostas de defesa discrepantes entre os dois genótipos e entre os tempos de coleta (12, 72 e 168 horas após inoculação). A resposta de indução dessas enzimas assemelha-se à defesa bifásica, para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Atividade enzimática; Mecanismos de defesa; Resistência sistêmica; Resposta em cascata.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200003

Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean
Provedor de dados: 86
Autores: Stolf-Moreira,Renata; Lemos,Eliana Gertrudes de Macedo; Abdelnoor,Ricardo Vilela; Beneventi,Magda Aparecida; Rolla,Amanda Alves Paiva; Pereira,Selma dos Santos; Oliveira,Maria Cristina Neves de; Nepomuceno,Alexandre Lima; Marcelino-Guimarães,Francismar Corrêa.
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Expression stability; RT-qPCR; Water deficit.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008