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A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
Provedor de dados: 48
Autores: Acharya,Arpan; Vaniawala,Salil; Shah,Parth; Parekh,Harsh; Misra,Rabindra Nath; Wani,Minal; Mukhopadhyaya,Pratap N.
BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p&amp;#8201;&lt;&amp;#8201;0.001). Lower limit of detection of the test protocol was 50...
Tipo: Journal article
Palavras-chave: HIV-1; Gag; SYBR Green; RT PCR.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100022