Consulta: creatorFacets:"Alhassan, A"
Registros recuperados: 3
Data/hora: 02/06/2024 12:47:57

The Babesia bovis BvP36 gene is expressed at least in the asexual stage of Babesia merozoite
Provedor de dados: 19
Autores: Khukhuu, A; Sivakumar, T; Alhassan, A; Ota, N; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Recently, Babesia bovis genome project identified a novel gene, which is homologous to the gene encoding sporozoite P36 antigen in Plasmodium parasites. In the present study, we isolated the P36 homologous gene from the genomic DNA of B. bovis and designated it as the BvP36 gene. The sequence of the BvP36 gene was identical to that reported previously. This gene was expressed in an Escherichia coli system to produce a recombinant protein (rBvP36). The rBvP36 protein was produced as a highly soluble form in E. coli and was detected by western blot analyses of sera collected from cattle experimentally infected with B. bovis and B. bigemina merozoites. In indirect immunofluorescence tests, sera collected from mice immunized with rBvP36 showed strong reactions...
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2385

Expression analysis of a Babesia bovis BboP67 gene homologous to the Theileria parva p67 gene
Provedor de dados: 19
Autores: Sivakumar, T; Khukhuu, A; Alhassan, A; Rezk Ali Aboulaila, M.
A fragment of a novel gene, designated as the BboP67 gene, of Babesia bovis, which had been recently reported as a homolog of the gene encoding a sporozoite-specific P67 antigen of Theileria parva, was isolated from the genomic DNA of B. bovis and expressed as a recombinant (rBboP67) in E. coli. The antigenicity of rBboP67 was evaluated using 102 and 68 field serum samples collected from cattle in Ghana and Mongolia, respectively. All of the B. bovis- and B. bigemina-infected sera, which had been identified by the species-specific antigens, rRAP-1/CTs, for B. bovis and B. bigemina in the standard ELISA, positively reacted with the rBboP67 in ELISA. While B. bovis-infected field serum samples recognized the rBboP67 in Western blot analysis, sera collected...
Palavras-chave: BboP67; Babesia bovis; Babesia bigemina; Sporozoite; Theileria parva.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2388

In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina
Provedor de dados: 19
Autores: Sivakumar, T; Aboulaila, M. R. A.; Khukhuu, A; Iseki, H; Alhassan, A; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
The in vitro inhibitory effect of fosmidomycin was evaluated against the asexual growth of bovine Babesia parasites (Babesia bovis and Babesia bigemina). In the cultures with these parasites, severe reduction of parasite growth was observed by the addition of 1.25 μg/ml of the final drug concentration. Under these conditions, B. bigemina was completely cleared until the third day of cultivation. On the other hand, B. bovis was completely eliminated on the fourth day when the concentration was 6.25 μg/ml in the culture. These parasites failed to grow again when a drug-free medium was substituted for the subsequent cultivation. The IC50 values of fosmidomycin against B. bovis were determined as 0.88 and 0.63 μg/ml in the in vitro cultures with...
Palavras-chave: Fosmidomycin; Babesia bovis; Babesia bigemina; Asexual growth; In vitro culture.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2235