Consulta: creatorFacets:"Alves,L.G."
Registros recuperados: 2
Data/hora: 02/06/2024 00:32:29

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora
Provedor de dados: 56
Autores: Silva,T.M.A.; Alves,L.G.; Queiroz,K.C.S. de; Santos,M.G.L.; Marques,C.T.; Chavante,S.F.; Rocha,H.A.O.; Leite,E.L.
The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fucan; Anticoagulant activity; Sulfated polysaccharides; Brown algae; Padina gymnospora.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400005

Heterofucans from Dictyota menstrualis have anticoagulant activity
Provedor de dados: 56
Autores: Albuquerque,I.R.L.; Queiroz,K.C.S.; Alves,L.G.; Santos,E.A.; Leite,E.L.; Rocha,H.A.O.
Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fucan; Fucoidan; Anticoagulant drugs; Brown algae; Antithrombotic agents.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200002