Consulta: creatorFacets:"Anzai,Eleine Kuroki"
Registros recuperados: 2
Data/hora: 05/06/2024 04:22:50

Leptospires detection in kidney, liver and uterus of cows slaughtered in Paraná State, Brazil
Provedor de dados: 58
Autores: Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda.
The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008

Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil
Provedor de dados: 58
Autores: Zacarias,Francielle Gibson da Silva; Vasconcellos,Silvio Arruda; Anzai,Eleine Kuroki; Giraldi,Nilson; Freitas,Julio Cesar de; Hartskeerl,Rudy.
In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Culture; Leptospirosis; Diagnosis; Cattle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400028