Consulta: creatorFacets:"Arantes,Eliane Candiani"
Registros recuperados: 10
Data/hora: 01/06/2024 04:22:57

Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion
Provedor de dados: 80
Autores: Shibao,Priscila Yumi Tanaka; Cologna,Camila Takeno; Morandi-Filho,Romualdo; Wiezel,Gisele Adriano; Fujimura,Patricia Tiemi; Ueira-Vieira,Carlos; Arantes,Eliane Candiani.
Abstract  Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules.  Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-seq; Rhinella schneideri; Toad secretion; Transcriptome; Illumina; Cutaneous secretion; Skin secretion; Toad protein.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100326

Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
Provedor de dados: 80
Autores: Boldrini-França,Johara; Pinheiro-Junior,Ernesto Lopes; Arantes,Eliane Candiani.
ABSTRACT  Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose clots and fibrinogen consumption. We report the functional and biological characterization of a recombinant thrombin-like serine protease from Crotalus durissus collilineatus, named rCollinein-1.  Methods: Heterologous expression of rCollinein-1 was performed in Pichia pastoris system according to a previously standardized protocol, with some modifications. rCollinein-1 was purified from the culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Serine proteases; Thrombin-like enzymes; Coagulation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100306

Antivenomic approach of different Crotalus durissus collilineatus venoms
Provedor de dados: 80
Autores: Oliveira,Isadora Sousa de; Pucca,Manuela Berto; Sampaio,Suely Vilela; Arantes,Eliane Candiani.
Abstract  Background: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian anticrotalic serum capacity in recognizing twenty-two Crotalus durissus collilineatus venoms, as well as their fractions.  Methods: The indirect enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate the efficacy of heterologous anticrotalic serum produced by Instituto Butantan (Brazil) in recognizing the twenty-two Crotalus durissus collilineatus venoms and the pool of them. Moreover, the venom...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snake venoms; Crotalus durissus collilineatus; Antivenomic; Crotalic antivenom; Individual variation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100601

Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom
Provedor de dados: 80
Autores: Cremonez,Caroline Marroni; Leite,Flávia Pine; Bordon,Karla de Castro Figueiredo; Cerni,Felipe Augusto; Cardoso,lara Aimê; Gregório,Zita Maria de Oliveira; Souza,Rodrigo Cançado Gonçalves de; Souza,Ana Maria de; Arantes,Eliane Candiani.
Abstract  Background  In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata.  Methods  We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lachesis muta rhombeata; Bushmaster; Natural antivenom; Antiophidic action; Soursop; Annona muricata L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100310

Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations
Provedor de dados: 80
Autores: Cologna,Camila Takeno; Rodrigues,Renata Santos; Santos,Jean; Pauw,Edwin de; Arantes,Eliane Candiani; Quinton,Loïc.
Abstract  Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectrometry allows, due to its high sensitivity, the study of venoms from species previously left aside, such as ants. Ant venoms are a complex mixture of compounds used for defense, predation or communication purposes. The venom from Neoponera ants, a genus restricted to Neotropical regions, is known to have cytolytic, hemolytic, antimicrobial and insecticidal activities. Moreover, venoms from several Neoponera species have been compared and differences in their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ant venom; Peptidomics; Ponericins; Venom comparison; Antimicrobial peptides.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100303

Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Provedor de dados: 80
Autores: Cordeiro,Francielle Almeida; Coutinho,Bárbara Marques; Wiezel,Gisele Adriano; Bordon,Karla de Castro Figueiredo; Bregge-Silva,Cristiane; Rosa-Garzon,Nathalia Gonsales; Cabral,Hamilton; Ueberheide,Beatrix; Arantes,Eliane Candiani.
Abstract  Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV).  Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lachesis muta rhombeata; Metalloprotease; Proteases; Snake venom.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100323

Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Provedor de dados: 80
Autores: Baldo,Mateus Amaral; Cunha,Alexandra Olimpio Siqueira; Godoy,Lívea Dornela; Liberato,José Luiz; Yoneda,Juliana Sakamoto; Fornari-Baldo,Elisa Correa; Ciancaglini,Pietro; Santos,Wagner Ferreira dos; Arantes,Eliane Candiani.
Abstract  Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest.  Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinella schneideri; Toad poison; Bufadienolides; Seizures; Neuroprotection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309

Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom
Provedor de dados: 80
Autores: Oliveira,Isadora Sousa de; Manzini,Rafaella Varzoni; Ferreira,Isabela Gobbo; Cardoso,Iara Aimê; Bordon,Karla de Castro Figueiredo; Machado,Ana Rita Thomazela; Antunes,Lusânia Maria Greggi; Rosa,José Cesar; Arantes,Eliane Candiani.
Abstract  Background: In recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. Disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ECM interactions. These molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogenesis. Therefore, disintegrin from Crotalus durissus collilineatus venom was isolated, structurally characterized and evaluated for its toxicity and ability to interfere with cell proliferation and migration in MDA-MB-231, a human breast cancer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus collilineatus; Non-RGD disintegrin; Cell migration; Cell adhesion; Human breast cancer; MDA-MB-231.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100319

Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins
Provedor de dados: 80
Autores: Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani.
Abstract  Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308

Tityus serrulatus envenoming in non-obese diabetic mice: a risk factor for severity
Provedor de dados: 80
Autores: Oliveira,Guilherme Honda de; Cerni,Felipe Augusto; Cardoso,Iara Aimê; Arantes,Eliane Candiani; Pucca,Manuela Berto.
Abstract  Background  In Brazil, accidents with venomous animals are considered a public health problem. Tityus serrulatus (Ts), popularly known as the yellow scorpion, is most frequently responsible for the severe accidents in the country. Ts envenoming can cause several signs and symptoms classified according to their clinical manifestations as mild, moderate or severe. Furthermore, the victims usually present biochemical alterations, including hyperglycemia. Nevertheless, Ts envenoming and its induced hyperglycemia were never studied or documented in a patient with diabetes mellitus (DM). Therefore, this is the first study to evaluate the glycemia during Ts envenoming using a diabetic animal model (NOD, non-obese diabetic).  Methods  Female mice (BALB/c...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Diabetes mellitus; Scorpion venom; NOD mice; Glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100319