Consulta: creatorFacets:"Arzul, Isabelle"
Registros recuperados: 120
Data/hora: 01/06/2024 01:03:00

Haemocytic neoplasia in Mediterranean mussels (Mytilus galloprovincialis) in the Slovene Adriatic Sea
Provedor de dados: 5
Autores: Gombac, Mitja; Sitar, Rosvita; Pogacnik, Milan; Arzul, Isabelle; Jencic, Vlasta.
The health status of cultured and wild Mediterranean mussels in the Slovene Sea has so far been unexplored. Initially, 1280 adult Mediterranean mussels (Mytilus galloprovincialis), 960 from a shellfish farm and 320 from natural beds, were collected over a one-year period. Water temperature, oxygenation and salinity were measured at each sampling. Mussels were measured and weighted to calculate the condition index and microscopically examined for the presence of haemocytic neoplasia. Haemocytic neoplasia was detected in 14 mussels (1.1%) with a higher prevalence in cultured mussels. Neoplastic cells singularly infiltrated the connective tissue, in small foci or diffusely. Necrosis and multifocal atrophy of digestive tubules were noticed in mussels with...
Tipo: Text
Palavras-chave: Haemocytic neoplasia; Mediterranean mussels; Mytilus galloprovincialis; Slovene Sea.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25512/23959.pdf

Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms.  Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf

One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence.
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in...
Tipo: Text
Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf

Infections à virus de type herpes chez les bivalves : large spectre d'hôte et détection au stade adulte
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan.
In 1972, Farley reported the presence of herpes-type viral particles in adult American oysters (Crassostrea virginica) for the first time. Since then, this pathogen has been detected in various marine bivalve species, usually associated with massive mortality events. To date, herpes-type viruses have been detected in the following species: C gigas, Ostrea edulis, O. angasi, Tiostrea chilensis, Ruditapes decussatus, R. philippinarum and most recently, Pecten maximus.
Tipo: Text
Palavras-chave: Pathogènes; Bivalves; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3266.pdf

Mass Mortalities Affecting Populations of the Yellow Clam     Amarilladesma mactroides     Along Its Geographic Range
Provedor de dados: 5
Autores: Vazquez, Nuria; Fiori, Sandra; Arzul, Isabelle; Carcedo, Cecilia; Cremonte, Florencia.
The yellow clam Amarilladesma mactroides (Deshayes, 1854) is considered a vulnerable species since the mid-1990s. Populations have experienced mass mortalities throughout their entire range of distribution (23–41°S) along exposed sandy beaches of Atlantic South America. Detrimental anthropogenic impacts have further contributed to failure of populations to make a recovery. To determine the factors involved in these events, density prior to a mortality event was calculated and live yellow clams encompassingmost of its geographic range distribution were analyzed histologically to describe parasites and pathologies. Moreover, moribund specimens were analyzed by molecular techniques to test for the presence of the virus OsHV-1. A mortality event was recorded...
Tipo: Text
Palavras-chave: Mass mortalities; Viral susceptibility; Amarilladesma mactroides; Pathologies.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00362/47283/47260.pdf

Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre.
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of...
Tipo: Text
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf

Le Réseau REPAMO : un outil de surveillance de la santé des mollusques marins élevés et sauvages
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Arzul, Isabelle; Robert, Maeva; Omnes, Emmanuelle; Chollet, Bruno; Renault, Tristan.
The Ifremer Repamo network (REseau de PAthologie des MOllusques) is the national surveillance network in charge of monitoring marine mollusc health on French coastlines. It is commissioned to enforce the official regulations and to provide a public service, under the supervision of the competent authority (DGAL Veterinary Directorate) in the Ministry.of Agriculture and Fisheries. Its goals are to prevent the introduction and the dissemination of infectious agents, in particular pathogens involved in reportable diseases, and to monitor the changes in the pathogens already present on the French coasts.
Tipo: Text
Palavras-chave: Network; Surveillance; Mollusks; Pathogens; Regulations.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6946.pdf

Can survival of European flat oysters following experimental infection with Bonamia ostreae be predicted using QTLs?
Provedor de dados: 5
Autores: Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie.
The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also...
Tipo: Text
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf

Polymorphism at the ITS and NTS Loci of Perkinsus marinus isolated from cultivated oyster Crassostrea corteziensis in Nayarit, Mexico and Phylogentic relationship to P. marinus along the Atlantic Coast
Provedor de dados: 5
Autores: Escobedo-fregoso, C.; Arzul, Isabelle; Carrasco, Noelia; Gutierrez-rivera, J. N.; Llera-herrera, Raul; Vazquez-juarez, R.
Prevalence of the protozoan Perkinsus spp. in the gills of the pleasure oyster Crassostrea corteziensis from two estuaries in Nayarit, Mexico, was measured. The protozoan was identified by PCR amplification of the internal transcribed spacer (ITS) region of the rDNA of Perkinsus spp. The pathogen was found in 92% of oysters from Boca de Camichín and 77% of oysters from Pozo Chino. ITS sequences characterized from C. corteziensis showed 96–100% similarity to Perkinsus marinus. The most frequent ITS sequence, (GenBank JQ266236) had 100% identity with the ITS locus of P. marinus from New Jersey, Maryland, South Carolina, and Texas, and the second most frequent observed sequence (GenBank JQ266240) was 100% identical to ITS sequences of P. marinus from New...
Tipo: Text
Palavras-chave: Crassostrea corteziensis; Perkinsus marinus; Internal transcribed spacer; Non-internal transcribed spacer; Aquatic disease.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00137/24811/22892.pdf

Bilan 2004 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Goubet, Anne; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Costantini, Louis; Masson, Jean-claude; Martin, Anne-genevieve.
Created in 1986, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91 /67/EEC and 95/70/EC and are a patt of the institutional tasks ofIfremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to survey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Banc de Granville and zone X). In 2004, no sample couId be analysed to these two areas because of the difficulty to obtain flat oysters (sparse oyster beds,...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00086/19702/17343.pdf

A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

Permanent Advisory Network for Diseases in Aquaculture (PANDA)
Provedor de dados: 5
Autores: Arzul, Isabelle; Ariel, E.; Hill, B.
The PANDA project aims to establish a permanent network of aquatic animal health specialists (researchers and diagnosticians) to provide a forum for the debate of issues concerning diseases in European aquaculture, and to communicate the results of these discussions to the European Commission with provision of advice on EU policy and legislation for aquatic animal health. PANDA consists of five scientific work packages: risk analysis, epidemiology, diagnosis, environmentally safe strategies for disease control, training in research and diagnostics. These work packages are lead by PANDA consortium members, supported by small task force of specialists, with wider input from experts registered in the PANDA network. Focused workshops and ongoing online...
Tipo: Text
Palavras-chave: Aquatic infectious diseses; Diagnosis; Risk analysis; PANDA; Animal health.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3320.pdf

Bilan 2015 du dispositif national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Fleury, Elodie; Robert, Stephane; Garcia, Celine; Arzul, Isabelle; Baillon, Laury; Bechemin, Christian; Canier, Lydie; Chollet, Bruno; Dechamps, Lucie; Dubreuil, Christine; Faury, Nicole; Francois, Cyrille; Godfrin, Yoann; Lapegue, Sylvie; Morga, Benjamin; Travers, Marie-agnes; Tourbiez, Delphine; Masson, Jean-claude; Verin, Francoise; Cordier, Remy; Gangnery, Aline; Louis, Wilfried; Mary, Charlotte; Normand, Julien; Penot, Julia; Cheve, Julien; Dagault, Francoise; Le Jolivet, Aurore; Le Gal, Dominique; Lebrun, Luc; Bellec, Gwenael; Bouget, Jean-francois; Cochennec-laureau, Nathalie; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Pepin, Jean-francois; Seugnet, Jean-luc; D'Amico, Florence; Maurer, Daniele; Le Gall, Patrik; Mortreux, Serge; Baldi, Yoann; Orsoni, Valerie; Bouchoucha, Marc; Le Roy, Valerian; Pouvreau, Stephane; Queau, Isabelle; Lamoureux, Alice.
Depuis 1992, la surveillance de la santé des mollusques marins du littoral français est assurée par le réseau de Pathologie des Mollusques (Repamo). Ses activités s’inscrivent dans le cadre de la Directive Européenne 2006/88/CE. Depuis son évaluation par la plateforme nationale d’épidémiosurveillance en santé animale en 2012, l’objectif de surveillance est la détection précoce des infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’année 2015 est la première année de transition pour laquelle un début d’évolution des modalités de surveillance de la santé des mollusques marins animées par l’Ifremer a été amorcé. Un dispositif hybride de surveillance a été mis en place, s’appuyant sur...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Mortalité; Maladie.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43486/42945.pdf

Recommendations for evaluation of the health status in cultured and wild shellfish: Perkinsus olseni infestation in clams as an example
Provedor de dados: 5
Autores: Miossec, Laurence; Arzul, Isabelle; Garcia, Celine; Soudant, Philippe; Francois, Cyrille; Leite, R. B.; Cancela, M. L.; De Blas, I.
Infectious diseases in exploited shellfish population cause economical problems world-wide. The characteristics of the disease in the population -i.e. origin, frequency, distribution, development- need to be well known to set up appropriate measures for protection and restoration of shellfish population health. The investigation of disease in a population is the basis of epidemiology. Quantitative investigations include studies, survey, surveillance with specific sampling strategies according to the objectives. European clams (Ruditapes decussatus) and Manila clams (Ruditapes philippinarum), two commercially valuable species, are infected by the protozoan Perkinsus olseni. This pathogen has been associated with epizootic outbreaks involving in heavy...
Tipo: Text
Palavras-chave: Recommendations; Epidemiology; Aquatic animal diseases; Pathology; Perkinsus olseni.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3326.pdf

Can Bonamia ostreae infect larvae of flat oysters Ostrea edulis ?
Provedor de dados: 5
Autores: Arzul, Isabelle; Robert, Maeva; Garcia, Celine; Chollet, Bruno; Langlade, Aime.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00448/55999/57503.pdf

Ostreid herpesvirus 1 infection in farmed Pacific oyster larvae Crassostrea gigas (Thunberg) in Korea
Provedor de dados: 5
Autores: Hwang, J. Y.; Park, J. J.; Yu, H. J.; Hur, Y. B.; Arzul, Isabelle; Couraleau, Yann; Park, M. A.
Tipo: Text
Palavras-chave: Crassostrea gigas; Korea; Ostreid herpesvirus 1; Pacific oyster.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00162/27276/26231.pdf

A literature review as an aid to identify strategies for mitigating ostreid herpesvirus 1 in Crassostrea gigas hatchery and nursery systems
Provedor de dados: 5
Autores: Rodgers, Chris; Arzul, Isabelle; Carrasco, Noèlia; Furones Nozal, Dolores.
An understanding of husbandry strategies and any associated risk factors is important for designing management control measures that can reduce mortality in Pacific oysters, Crassostrea gigas, caused by ostreid  herpesvirus 1 (OsHV-1). The type of culture facility can be considered in relation to the potential pathways that could lead to the entry of a pathogen and its survival. In addition, the animal host (e.g. age, physiological state,  selective breeding programmes), husbandry procedures (e.g. stocking density), the pathogen itself (e.g. pathogenicity, virulence) and environmental effects (e.g. temperature) represent other relevant interconnected factors. However, all these factors provide valuable background information for outlining the mitigation...
Tipo: Text
Palavras-chave: Crassostrea gigas; Husbandry; Management strategies; Ostreid herpesvirus 1; Oyster herpesvirus; Risk factors.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00474/58581/61146.pdf

Viruses infecting marine molluscs
Provedor de dados: 5
Autores: Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan.
Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing...
Tipo: Text
Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf

New Perspective On The Haplosporidian Parasites Of Molluscs
Provedor de dados: 5
Autores: Arzul, Isabelle; Carnegie, Ryan B.
The protist phylum Haplosporidia comprises over 40 described species with representatives infecting a range of mollusc hosts, including several ecologically and economically significant pathogens. Continuing exploration of haplosporidian diversity has added ten new species in recent years and brought the phylogenetics of the group into somewhat clearer focus, with monophyletic Bonamia and Minchinia lineages continuing to be supported. However, the addition of new sequences to phylogenetic analyses has left the paraphyletic genus Haplosporidium’s picture less resolved. It is not clear that even two genera will be enough to accommodate the species presently drawn to the Haplosporidium regions of the haplosporidian tree. In this review, we summarize recent...
Tipo: Text
Palavras-chave: Haplosporidium; Bonamia; Minchinia; MSX disease; Haplosporidiosis; Bonamiosis.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00275/38619/37149.pdf

Le réseau Repamo : un outil de surveillance de la santé des mollusques marins élevés et sauvages (acte)
Provedor de dados: 5
Autores: Francois, Cyrille; Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Robert, Maeva; Ferrand, Sylvie; Chollet, Bruno; Renault, Tristan.
Created in 1992, the Repamo network (REseau de PAthologie des MOllusques – Mollusc Pathology Network) is in charge of the surveillance and monitoring of the marine molluscs health status along the French coast. It has a regulatory mission (European legislation 91/67 and 95/70) as well as a public service activity under the supervision of the Ministry of Agriculture and Fisheries through the DPMA. It aims at preventing the introduction and propagation of pathogenic agents, in particular the noticeable agents, and monitoring the evolution of those already present on the national territory. These activities are part of the institutional missions of the Ifremer. The missions of the network are the following: 1) surveillance of the evolution of noticeable...
Tipo: Text
Palavras-chave: Mortalités; Maladies; Pathologie; Mollusques; Réseau surveillance; REPAMO.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3715.pdf

Avis sur la détection de parasites du genre Marteilia proche de l’espèce Marteilia cochillia chez les coques
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Serpin, Delphine; Noyer, Mathilde; Canier, Lydie; Bechemin, Christian.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00602/71383/81205.pdf

Detection of Bonamia sp., infecting flat oysters, Ostrea puelchana (d’Orbigny, 1842) in San Matias Gulf (NW Patagonia, Argentina) based on 18S Small sub-unit and ITS Segment sequence
Provedor de dados: 5
Autores: Kroeck, Marina; Arzul, Isabelle; Robert, Maeva; Carrasco, Noelia.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00448/56000/57504.pdf

Study of interactions between flat oyster, Ostrea edulis, and the parasite Bonamia ostreae using flow cytometry and suppression subtractrive hybridization (SSH)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Gagnaire, Beatrice; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. Control of bonamiosis requires a good knowledge of the parasite life cycle including host-parasite interactions. In this study, we investigated interactions between parasites and haemocytes by flow cytometry and Suppression Subtractive Hybridization (SSH). In both approaches, analyses were performed after 2 hours of contact between haemocytes and parasites purified from highly infected flat oysters. Flow cytometry analyses consisted in testing haemocyte activities including esterase activities, reactive oxygen species (ROS) production and phagocytosis after contact with live parasites and parasites inactivated by...
Tipo: Text
Palavras-chave: SSH; Suppression subtractive hybridization; Cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3367.pdf

Suivi des gisements naturels de mollusques dans le cadre du réseau Repamo
Provedor de dados: 5
Autores: Garcia, Celine; Francois, Cyrille; Arzul, Isabelle; Miossec, Laurence; Joly, Jean-pierre; Robert, Maeva; Chollet, Bruno; Ferrand, Sylvie.
A monitoring of the natural and high density populations of commercial molluscs is performed on an annual basis within the scope of the Repamo mollusc pathology monitoring network The aim of this surveillance is (1) to monitor the health status of the molluscs along the French coast and to produce a reference picture, and (2) to study the evolution of the noticeable pathogenic agents existing on the national territory. Different sampling strategies have been introduced for the past 15 years; each strategy is based on an active monitoring including the systematic study of natural mollusc deposits. Indeed, the natural and high density mollusc populations are interrelated and pathogenic agents can be and have been exchanged between these populations with...
Tipo: Text
Palavras-chave: Haliotis tuberculata; Ostrea edulis; Population sauvage; Elevage; Mollusques; REPAMO; Réseau surveillance.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3720.pdf

Summer mortality outbreaks of French Pacific oysters Crassostrea gigas in 2008 and 2009
Provedor de dados: 5
Autores: Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie.
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August....
Tipo: Text
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf

Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf

Evolutionary Origins of Rhizarian Parasites
Provedor de dados: 5
Autores: Sierra, Roberto; Canas-duarte, Silvia J.; Burki, Fabien; Schwelm, Arne; Fogelqvist, Johan; Dixelius, Christina; Gonzalez-garcia, Laura N.; Gile, Gillian H.; Slamovits, Claudio H.; Klopp, Christophe; Restrepo, Silvia; Arzul, Isabelle; Pawlowski, Jan.
The SAR group (Stramenopila, Alveolata, Rhizaria) is one of the largest clades in the tree of eukaryotes and includes a great number of parasitic lineages. Rhizarian parasites are obligate and have devastating effects on commercially important plants and animals but despite this fact, our knowledge of their biology and evolution is limited. Here, we present rhizarian transcriptomes from all major parasitic lineages in order to elucidate their evolutionary relationships using a phylogenomic approach. Our results suggest that Ascetosporea, parasites of marine invertebrates, are sister to the novel clade Apofilosa. The phytomyxean plant parasites branch sister to the vampyrellid algal ectoparasites in the novel clade Phytorhiza. They also show that...
Tipo: Text
Palavras-chave: Rhizaria; Parasites; Phylogenomics.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00320/43156/44988.pdf

Avis sur la détection d'agents infectieux réglementés chez les mollusques marins en France au cours du second semestre 2018
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Canier, Lydie; Combette, Agnes; Bechemin, Christian.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58898/81058.pdf

Transmission expérimentale du virus de type herpes chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Arzul, Isabelle.
Au cours de l'été 1991, d'importantes mortalités de larves d'huître creuse, Crassostrea gigas, ont été observées dans des écloseries, simultanément en France (Comps et Cochennec, 1993) et en Nouvelle-Zélande (Hine &amp; al., 1992).  Les examens histologiques ainsi que des examens réalisés en microscopie électronique à transmission d'échantillons de larves présentant des mortalités ont permis de détecter la présence de particules virales associées à des lésions cellulaires.  D'après la morphologie, la taille, les localisations intranucléaires et cytoplasmiques du virus, il est possible d'envisager son appartenance à la famille des Herpesviridae.  De nouvelles mortalités sporadiques ont été observées au cours des étés suivants sur des lots de larves élevées...
Tipo: Text
Palavras-chave: Virus; Herpesviridae; Bivalve; Crassostrea gigas; Etiologie; Epidémiologie; Diagnostic; Reproduction infection.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00106/21711/19289.pdf

An eDNA/eRNA‐based approach to investigate the life cycle of non‐cultivable shellfish micro‐parasites: the case of   Bonamia ostreae   , a parasite of the European flat oyster   Ostrea edulis
Provedor de dados: 5
Autores: Mérou, Nicolas; Lecadet, Cyrielle; Pouvreau, Stephane; Arzul, Isabelle.
Environmental DNA approaches are increasingly used to detect microorganisms in environmental compartments, including water. They show considerable advantages to study non‐cultivable microorganisms like Bonamia ostreae, a protozoan parasite inducing significant mortality in populations of flat oyster Ostrea edulis . Although B. ostreae development within the host has been well described, questions remain about its behaviour in the environment. As B. ostreae transmission is direct, seawater appears as an interesting target to develop early detection tools and improve our understanding of disease transmission mechanisms. In this context, we have developed an eDNA/eRNA approach allowing detecting and quantifying B. ostreae 18S rDNA/rRNA as well as monitoring...
Tipo: Text
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00636/74800/75174.pdf

Project ROMEO : E. C. n° FA-S2 9052 - Research objectives : Mortality in European Oysters - Final Report
Provedor de dados: 5
Autores: Renault, Tristan; Arzul, Isabelle.
Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,...
Tipo: Text
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf

Molecular characterization of parasites of the genus Perkinsus present in clams from French producing areas
Provedor de dados: 5
Autores: Arzul, Isabelle; Michel, Justine; Chollet, Bruno; Robert, Maeva; Miossec, Laurence; Garcia, Celine; Francois, Cyrille.
Production of clams is the third most important bivalve production in France and was estimated at 3400 metric tons in 2001 among which 60% came from aquaculture and 40% from fisheries. Surveillance of clam diseases mainly relies on histology and thioglycolate culture, two techniques allowing detection of parasites of the genus Perkinsus but not specific enough to determine the parasite species. Based on a previous study aiming at estimating distribution and prevalence of perkinsosis in France, we selected 34 infected clams from 4 different producing areas in order to characterize the parasite species. Molecular characterization was done by PCR-RFLP targeting the ITS region as described by Abollo et al. (2006). 16 PCR products were cloned and 10 to 20...
Tipo: Text
Palavras-chave: French producing areas; Clams; Perkinsus olseni; Parasite.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6269.pdf

Inventaire, diagnostic écologique et restauration des principaux bancs d’huitres plates en Bretagne : le projet FOREVER
Provedor de dados: 5
Autores: Pouvreau, Stephane; Cochet, Hélène; Fabien, Aurélie; Arzul, Isabelle; Lapegue, Sylvie; Gachelin, Sonia; Salaun, Benoit.
L’huitre plate, la seule huitre native des côtes européennes, est une espèce emblématique de Bretagne. Convoitée depuis l'Antiquité, sur-pêchée au 19ème siècle, fleuron de l'ostréiculture au 20ème, elle est désormais menacée de disparition à l'aube du 21ème. Sous l'impulsion de la commission OSPAR mais aussi dans un contexte grandissant de crise écologique et d'érosion de la biodiversité imposant une nécessaire conservation et/ou restauration de nos patrimoines naturels, une alliance européenne réunissant biologistes et gestionnaires a été créée en novembre 2017 à Berlin sous l'égide de l'Agence Allemande de Conservation de la Nature (BFN) avec un objectif commun: favoriser le retour de l'huitre plate sur les côtes européennes. Cette alliance pour la...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00683/79506/82154.pdf

Bonamia ostreae and Ostrea edulis : a stable host-parasite system in France?
Provedor de dados: 5
Autores: Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production...
Tipo: Text
Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf

Synthèse PERLE. Programme d’Expérimentation et de recherche sur L’huître plate ostrea Edulis
Provedor de dados: 5
Autores: Hussenot, Mathieu; Pouvreau, Stephane; Duchene, Julie; Freulon, Henry; Arzul, Isabelle; Lapegue, Sylvie.
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce document présente une synthèse des principaux résultats du programme PERLE et ouvre de multiples perspectives pour les années à venir.
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00249/36060/34610.pdf

Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text
Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text
Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf

REPAMO: a surveillance tool for mollusc health
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva.
At the present time losses due to diseases are one of main factors limiting the aquaculture development. Unceasing increase of exchanges between different countries favours the risk of introduction and propagation of new pathogen agents. At the beginning of the 90’s the European Union laid the surveillance of mollusc health inside each Member State in order to protect the safety of exchanges. Indeed few measures are available to protect molluscs from diseases. Only the prophylactic approach is possible; this approach is based on a zoosanitary surveillance of mollusc populations and/or a development of resistant populations against some diseases. Created in 1986, the French network for surveillance and monitoring of mollusc health (REPAMO) ensures the...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00442/55334/56826.pdf

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio.
Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland....
Tipo: Text
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf

Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin.
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members...
Tipo: Text
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf

PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3
Provedor de dados: 5
Autores: Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf

Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
Provedor de dados: 5
Autores: Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf

Evidence for interspecies transmission of oyster herpesvirus in marine bivalves
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text
Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf

Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response...
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00454/56540/58246.pdf

Detection of Type 1 Ostreid Herpes variant (OsHV-1 mu var) with no associated mortality in French-origin Pacific cupped oyster Crassostrea gigas farmed in Italy
Provedor de dados: 5
Autores: Dundon, William G.; Arzul, Isabelle; Omnes, Emmanuelle; Robert, Maeva; Magnabosco, Cristian; Zambon, Michela; Gennari, Lorenzo; Toffan, Anna; Terregino, Calogero; Capua, Ilaria; Arcangeli, Giuseppe.
In April 2010, the sampling of juvenile Pacific cupped oysters originating from France was undertaken from a farm located off the coast of the Marche region in Italy. Samples were sent to the national reference laboratory for mollusc diseases for bacteriological, histological, electron microscopical and molecular analysis. Classical and Real-Time PCR indicated the presence of the microvariant (OsHV-1 mu var) of Type 1 Ostreid Herpes virus (OsHV-1) despite the absence of clinical and pathological signs normally associated with the presence of this variant. Further molecular and sequencing analysis revealed the presence of OsHV-1 par and OsHV-1 DNA in one oyster indicating that simultaneous detection of both viruses is possible. This is the first report of...
Tipo: Text
Palavras-chave: Pacific cupped oyster; Ostreid Herpes virus 1 mu var; PCR; Italy.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14585/11968.pdf

Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia
Provedor de dados: 5
Autores: Lopez Flores, L; Suarez Santiago, V; Longet, D; Saulnier, Denis; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and...
Tipo: Text
Palavras-chave: Molecular phylogeny; Actin gene; Ostrea edulis parasite; Bonamia ostreae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3562.pdf

Les surmortalités des naissains d’huîtres creuses, Crassostrea gigas : acquis des recherches en 2010
Provedor de dados: 5
Autores: Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel.
Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf

Bilan 2007 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Ravel, Christophe.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de deux Directives Européennes, les Directives 91 /67/CEE et 95/70/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae et la Marteiliose à Marteilia refringens chez l'huître...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Mollusques; Coquillages; Santé.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00086/19708/17349.pdf

Sensitivity of mortality reporting by the French oyster farmers
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Mandard, Y. V.; Peroz, C.; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Renault, Tristan.
As diseased shellfish show only very seldom symptoms, any mortality event can be a disease suspicion. In theory, monitoring of mortality events in shellfish can thus provide a tool for early detection of infectious diseases in order to implement effective control measures. This consists in passive mortality event reporting by shellfish farmers to the local competent authority. Sensitivity of the detection of mortality events is a key quality indicator for this type of surveillance. Since 2008, increased mortality outbreaks occur in Pacific oysters Crassostrea gigas in France, leading to economic losses. It is important to evaluate how much the outbreaks have altered the quality indicators of the reporting system and if this modification is long-standing,...
Tipo: Text
Palavras-chave: Early detection; Passive surveillance; Aquaculture; Capture-recapture methods.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00102/21343/18966.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Bilan 2009 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Cobret, Laetitia; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Breerette, Stephane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Banamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19711/17352.pdf

Delta de l'Ebre is a natural bay model for Marteilia spp. (Paramyxea) dynamics and life-cycle studies
Provedor de dados: 5
Autores: Carrasco, N; Arzul, Isabelle; Berthe, Franck; Fernandez Tejedor, M; Durfort, M; Furones, M.
Marteilia spp. are paramyxean parasites that affect several bivalve species of economic interest, such as Ostrea edulis and Mytilus galloprovincialis. Certain aspects of Marteilia spp., such as their life cycle and host affinity and infection dynamics, still remain unknown. The 'Delta de l'Ebre' constitutes a natural model for the study of the life cycle of the parasite Marteilia, since uninfected mussels and flat oysters immersed in the bays can become infected. This, along with the geographical and ecological characteristics of the bays, make it a very interesting location to study the Marteilia life cycle. Preliminary results concerning marteiliosis, mainly in mussels, such as prevalence dynamics, infectious periods, host affinity and host intermediate...
Tipo: Text
Palavras-chave: Ebre delta bays; Host specificity; Intermediate host; Mytilus galloprovincialis; Paramyxea; Marteilia.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3880.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: 5
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011
Provedor de dados: 5
Autores: Garcia, Celine; Haond, Christophe; Chollet, Bruno; Nerac, Mirella; Omnes, Emmanuelle; Joly, Jean-pierre; Dubreuil, Christine; Serpin, Delphine; Langlade, Aime; Le Gal, Dominique; Terre-terrillon, Aouregan; Courtois, Olivier; Guichard, Benjamin; Arzul, Isabelle.
Background: Microcell parasites are small intracellular protozoans mostly detected in molluscs and can be associated with mortalities. In 2010 and 2011, strong increases in mortality events were reported in different wild beds of the wedge clam Donax trunculus Linnaeus, along the Atlantic coast of France and the presence of potential pathogens, including microcells, was investigated. Methods: Clams collected in different beds showing mortality were examined by histology. Based on histological observations, confirmatory analyses were carried out, including transmission electron microscopy (TEM) and molecular characterization. Results: Histological analyses revealed the presence of small protozoans similar to microcell parasites in different tissues of Donax...
Tipo: Text
Palavras-chave: Mikrocytos veneroides n. sp.; Mikrocytos donaxi n. sp.; Donax trunculus; Mortality; Microcell parasite.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00428/53989/55149.pdf

Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa
Provedor de dados: 5
Autores: Prado-alvarez, Maria; Couraleau, Yann; Chollet, Bruno; Tourbiez, Delphine; Arzul, Isabelle.
Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where...
Tipo: Text
Palavras-chave: Bonamia exitiosa; Bonamia ostreae; Actin; Haplosporidia; Whole-Genome Amplification.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00277/38871/37410.pdf

Development of a TaqMan PCR assay for the detection of Bonamia species
Provedor de dados: 5
Autores: Corbeil, Serge; Arzul, Isabelle; Diggles, Ben; Heasman, Mike; Chollet, Bruno; Berthe, Franck; Crane, Mark St. J.
The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New...
Tipo: Text
Palavras-chave: Oyster; Real time TaqMan PCR assay; Bonamia spp.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1980.pdf

Infection à virus de type Herpes chez les bivalves : transmission expérimentale et induction de l'infection
Provedor de dados: 5
Autores: Arzul, Isabelle.
Depuis 1991, d'importantes mortalités sont observées chez des lots de larves et de naissain d'huître creuse, Crassostrea gigas, mais également sur des lots de larves d'autres espèces de bivalve telles que, l'huître plate, Ostrea edulis, les palourdes, Ruditapes decussatus et Ruditapes philippinarum. Ces épizooties se produisent plus généralement à fortes températures en écloserie-nurserie et en période estivale dans le milieu naturel.  Les techniques de détection utilisées jusqu'alors pour rechercher ces infections virales, histologie et microscopie électronique à transmission, paraissent assez inadaptées, tant du fait de leur sensibilité, du temps qu'elles nécessitent et de leur coût. Aussi, le laboratoire IFREMER de Ronce les Bains (Charente-Maritime) a...
Tipo: Text
Palavras-chave: Mollusques; Bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Herpèsvirus; Infection; Expérimentation.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00108/21957/19551.pdf

Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf

Bonamia parasites: a rapidly changing perspective on a genus of important mollusc pathogens
Provedor de dados: 5
Autores: Engelsma, Marc; Culloty, Sarah C.; Lynch, Sharon A.; Arzul, Isabelle; Carnegie, Ryan B.
Organisms of the genus Bonamia are intracellular protistan parasites of oysters. To date, 4 species have been described (B. ostreae, B. exitiosa, B. perspora and B. roughleyi), al though the status of B. roughleyi is controversial. Introduction especially of B. ostreae and B. exitiosa to naïve host populations has been shown to cause mass mortalities in the past and has had a dramatic impact on oyster production. Both B. ostreae and B. exitiosa are pathogens notifiable to the World Organisation for Animal Health (OIE) and the European Union. Effective management of the disease caused by these pathogens is complicated by the extensive nature of the oyster production process and limited options for disease control of the cultured stocks in open water. This...
Tipo: Text
Palavras-chave: Bonamia ostreae; Bonamia exitiosa; Oyster disease; Phylogenetics; Geographical spread; Diagnostics; Epizootiology; Host-parasite interaction; Bonamiosis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00200/31169/29568.pdf

Key outcomes from VIVALDI on preventing and mitigating bivalve diseases
Provedor de dados: 5
Autores: Arzul, Isabelle; Bass, David; Figueras, Antonio; Lamy, Jean-baptiste; Paillard, Christine; Furones, Dolors.
Since 2016, VIVALDI project, supported by the European Union H2020 programme, has aimed to improve the sustainability and competitiveness of the European shellfish industry, which has been hit by a growing number of mortality cases over the recent years. To this end, tools and strategies to better prevent and mitigate the impact of bivalve diseases have been developed through 6 work packages:  - In the 1st work package, the diversity and lifecycles of some bivalve pathogens have been investigated. Environmental-based approaches such as passive sensors or the use of magnetic beads and electrochemical biosensors could be useful for pathogen surveillance and the development of early warning systems.  - In the 2nd work package, some key mechanisms involved in...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00603/71518/69938.pdf

New evidence for the involvement of Paracartia grani (Copepoda, Calanoida) in the life cycle of Marteilia refringens (Paramyxea)
Provedor de dados: 5
Autores: Boyer, S.; Chollet, Bruno; Bonnet, D.; Arzul, Isabelle.
The dynamics of the protozoan parasite Marteilia refringens was studied in Thau lagoon, an important French shellfish site, for 1 year in three potential hosts: the Mediterranean mussel Mytilus galloprovincialis (Mytiliidae) the grooved carpet shell Ruditapes decussatus (Veneriidae) and the copepod Paracartia grani (Acartiidae). Parasite DNA was detected by PCR in R. decussatus. In situ hybridisation showed necrotic cells of M. refringens in the digestive epithelia of some R. decussatus suggesting the non-involvement of this species in the parasite life cycle. In contrast, the detection of M. refringens in mussels using PCR appeared bimodal with two peaks in spring and autumn. Histological observations of PCR-positive mussels revealed the presence of...
Tipo: Text
Palavras-chave: Marteiliosis; Marteilia refringens; Parasite life cycle; Mytilus galloprovincialis; Ruditapes decussatus; Paracartia grani; PCR; In situ hybridisation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26795/25532.pdf

Contribution to the understanding of the cycle of the protozoan parasite Marteilia refringens
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Boyer, S.; Bonnet, D.; Gaillard, Juliette; Baldi, Yoann; Robert, Maeva; Joly, Jean-pierre; Garcia, Celine; Bouchoucha, Marc.
The paramyxean parasite Marteilia refringens infects several bivalve species including European flat oysters Ostrea edulis and Mediterranean mussels Mytilus galloprovincialis. Sequence polymorphism allowed definition of three parasite types ‘M’, ‘O’ and ‘C’ preferably detected in oysters, mussels and cockles respectively.Transmission of the infection from infected bivalves to copepods Paracartia grani could be experimentally achieved but assays fromcopepods to bivalves failed. In order to contribute to the elucidation of the M. refringens life cycle, the dynamics of the infection was investigated in O. edulis, M. galloprovincialis and zooplankton over one year in Diana lagoon, Corsica (France). Flat oysters appeared non-infected while mussels were infected...
Tipo: Text
Palavras-chave: Bivalves; Marteilia refringens; Copepods; Paracartia latisetosa; Zooplankton; Parasite life cycle.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00165/27635/25768.pdf

Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle.
In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
Tipo: Text
Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf

Ostrea conchaphila : a natural host of Bonamia ostreae ?
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Garcia, Celine; Robert, Maeva; Joly, Jean-pierre; Miossec, Laurence; Berthe, Franck.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00448/55998/57502.pdf

Herpèsvirus infectant les bivalves marins : détection, génome et transmission
Provedor de dados: 5
Autores: Arzul, Isabelle.
Since 1991, high mortality rates of larvae and juveniles of different bivalve mollusc species associated with herpesvirus infections have been reported in different countries. The aim of this study was to determine if more than one helpesvirus species was responsible for these infections or just one species exhibiting polymorphism. The investigations concentrated on the analysis of the virus genome, studies of virus transmission in different mollusc species and on the potential role of bivalve adults in the occurrence of infections. The analysis of various target sequences within the virus genome present in infected larvae samples demonstrated the presence of polymorphism. More notably a variant of the virus was identified and the variation appears...
Tipo: Text
Palavras-chave: Herpèsvirus; Bivalves; Transmission; Polymorphisme; OsHV-1; Diagnostic; Herpesviruses; Bivalvia; Transmission; Polymorphism; OsHV-1; Diagnostic.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00105/21675/19253.pdf

Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cecile.
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under...
Tipo: Text
Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf

Space-time clustering of mortality notifications in Pacific oysters of Charente sluices, France, 2008-2010
Provedor de dados: 5
Autores: Lupo, Coralie; Mandard, Yann-vari; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Renault, Tristan; Bareille, Nathalie.
Current monitoring of exceptional mortality events of oysters in France consists in passive surveillance, leading to delayed investigation of causation by scientists and management by policy makers. Early detection of clusters of mortality events in time and space would increase possibilities for their investigation and management. A space-time cluster analysis of existing mortality notifications in Pacific oysters was conducted in France, Charente sluices, from 2008 to 2010. As an example of potential demographic confounder of clustering, the analyses were adjusted on oyster age. Several clusters were identified in space and time each studied year. Patterns of mortality notifications were consistent with the introduction and spread of a contagious disease...
Tipo: Text
Palavras-chave: Notification; Oyster; Mortality; Cluster analysis; Saptiotemporal.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00058/16915/14393.pdf

Bilan 2012 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER.   L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités...
Tipo: Text
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf

Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases.  Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their...
Tipo: Text
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf

Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks
Provedor de dados: 5
Autores: Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed....
Tipo: Text
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf

Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie.
We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage...
Tipo: Text
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf

Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006
Provedor de dados: 5
Autores: Garcia, Celine; Thebault, Anne; Degremont, Lionel; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Ferrand, Sylvie; Kerdudou, Nolwenn; Renault, Tristan.
Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15110/12572.pdf

Induction of apoptosis by UV in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that...
Tipo: Text
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf

Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
Provedor de dados: 5
Autores: Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle.
Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to...
Tipo: Text
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf

Bilan 2008 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf

Asari clam (Ruditapes philippinarum) in France: history of an exotic species 1972 – 2015
Provedor de dados: 5
Autores: De Montaudouin, Xavier; Arzul, Isabelle; Caill-milly, Nathalie; Khayati, Alice; Labrousse, Jean-michel; Lafitte, Céline; Paillard, Christine; Soudant, Philippe; Goulletquer, Philippe.
In 1972, France underwent an oyster (Crassostrea angulata) crisis and urgently needed to diversify its aquaculture. Thus, Asari clam (Ruditapes philippinarum) was introduced at that time for aquaculture purpose, concomitantly with the introduction of Pacific oyster (Crassostrea gigas). All Asari clam adults and spat originated from the same area (Puget Sound, WA, USA). After a promising start and the implementation of a national Research &amp; Development program, Asari clam culture rapidly faced a series of concomitant handicaps: firstly, in spite the fact that cultural practices were optimized and locking points addressed, leasing ground availability was limited during the 1980s’ due to certain reluctance from oyster farmers to share their leases and/or...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; France; Aquaculture; Professional fishing; Diseases; Environmental stressors.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00366/47767/47817.pdf

Défis de la surveillance des maladies chez les coquillages marins en France
Provedor de dados: 5
Autores: Lupo, Coralie; Francois, Cyrille; Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Renault, Tristan.
The basic concepts and objectives of animal disease surveillance are common to all types of animal production. Yet, the procedures for implementation have been mostly developed for terrestrial animal productions. Disease surveillance in marine shellfish calls for specific measures in view of the peculiarities of marine biology and rearing techniques. The main objective of disease surveillance in marine shellfish is to detect as early as possible exotic and newly emerging diseases. With this objective in mind, a national surveillance network for surveillance of mollusc diseases called Repamo has been developed. It combines passive reporting and risk-based surveillance.
Tipo: Text
Palavras-chave: Surveillance; Coquillage; Milieu marin; Détection précoce; Epidémiologie; Surveillance; Shellfish; Marine environment; Early detection.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00109/22023/19653.pdf

Establishing causal link between an infectious agent and mortalities in marine molluscan aquaculture on the example of Bonamia ostreae and Herpèsvirosis in oysters : proposal of a causal grid analysis
Provedor de dados: 5
Autores: Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan.
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes...
Tipo: Text
Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf

Interactions bivalves-protozoaires parasites : un équilibre dynamique
Provedor de dados: 5
Autores: Arzul, Isabelle.
Les bivalves jouent un rôle important dans le fonctionnement et la structure des écosystèmes marins et présentent un intérêt économique non négligeable en tant que ressources alimentaires dans de nombreux pays. En raison de leur impact négatif sur les populations de mollusques sauvages et d’élevage, les parasites protozoaires des genres Bonamia, Marteilia et Perkinsus font l’objet de programmes de surveillance et de travaux de recherche visant à mieux décrire leur diversité et leurs interactions avec leurs hôtes. Les travaux que nous avons réalisés sur ces parasites protozoaires visent à mieux comprendre pourquoi ces interactions, apparemment stables, sont sans cesse réajustées et pourquoi l’équilibre d’un système hôte-parasite est dynamique. Les résultats...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00239/34983/33503.pdf

REPAMO : a French network for the surveillance of molluscs health
Provedor de dados: 5
Autores: Guichard, Benjamin; Garcia, Celine; Joly, Jean-pierre; Lupo, Coralie; Chollet, Bruno; Robert, Maeva; Omnes, Emmanuelle; Arzul, Isabelle.
The REPAMO is a French Network for the surveillance of Mollusc diseases that notably meets the obligations of the European Directive 2006/088/EC. It is entirely funded by the French State. The coordination of this network entrusted to the Institut Français de Recherche pour l`Exploitation de la Mer since 1986 aims at monitoring the notifiable endemic diseases, detecting the emergence of exotic or new pathogens and ensuring a minimum level of surveillance of the general health condition of molluscs. Beside IFREMER, different partners are involved in the surveillance of mollusc health at the national level: the Departmental direction for territories and sea who are the local representatives of the competent authority and the producers who have the obligation...
Tipo: Text
Palavras-chave: Surveillance; Mollusc; Pathology; Network.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00036/14768/12077.pdf

Molecular characterisation of an Australian isolate of Bonamia exitiosa
Provedor de dados: 5
Autores: Corbeil, Serge; Arzul, Isabelle; Robert, Maeva; Berthe, Franck; Cochennec, Nathalie; Crane M, Mark.
An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.
Tipo: Text
Palavras-chave: Small subunit rRNA operon; Flat oyster; Ostrea angasi; Bonamia species.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1981.pdf

New insights in flat oyster Ostrea edulis resistance against the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line...
Tipo: Text
Palavras-chave: Protozoan; Ostrea edulis; Flat oyster; Transcriptomic; Gene expression.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00071/18218/15790.pdf

Bilan 2010 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf

Recommended reporting standards for test accuracy studies of infectious diseases of finfish, amphibians, molluscs and crustaceans: the STRADAS-aquatic checklist
Provedor de dados: 5
Autores: Gardner, Ian A.; Whittington, Richard J.; Caraguel, Charles G. B.; Hick, Paul; Moody, Nicholas J. G.; Corbeil, Serge; Garver, Kyle A.; Warg, Janet V.; Arzul, Isabelle; Purcell, Maureen K.; Crane, Mark St J.; Waltzek, Thomas B.; Olesen, Niels J.; Gallardo Lagno, Alicia.
Complete and transparent reporting of key elements of diagnostic accuracy studies for infectious diseases in cultured and wild aquatic animals benefits end-users of these tests, enabling the rational design of surveillance programs, the assessment of test results from clinical cases and comparisons of diagnostic test performance. Based on deficiencies in the Standards for Reporting of Diagnostic Accuracy (STARD) guidelines identified in a prior finfish study (Gardner et al. 2014), we adapted the Standards for Reporting of Animal Diagnostic Accuracy Studies—paratuberculosis (STRADAS-paraTB) checklist of 25 reporting items to increase their relevance to finfish, amphibians, molluscs, and crustaceans and provided examples and explanations for each item. The...
Tipo: Text
Palavras-chave: Reporting standards; Sensitivity; Specificity; Finfish; Amphibians; Molluscs; Crustaceans; STRADAS-paraTB; Diagnostic validation.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00334/44505/44191.pdf

VIVALDI, A H2020 European Project aiming at preventing and mitigating farmed bivalve diseases
Provedor de dados: 5
Autores: Arzul, Isabelle; Feist, Steve; Figueras, Antonio; Lapegue, Sylvie; Paillard, Christine; Furones, Dolors.
The European shellfish industry enjoys a privileged position on the global scene. Its social dimension is essential, as it employs a high number of people in more than 8000 companies, mostly micro-companies. Shellfish production in Europe is little diversified and mainly relies on the industrially produced mussels, oysters and clams. Over the recent years, this sector has grown more slowly than other fish farming sectors, notably because it depends a great deal on the environmental quality and the emergence of diseases. Mortality events, linked to pathogen organisms such as viruses, bacteria and parasites (protozoa), tend to weaken the production’s sustainability. In this context, the European project VIVALDI (PreVenting and mItigating farmed biVALve...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00352/46307/45975.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro (Diaporama)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Intéraction; Hémocytes; Ostrea edulis; Bonamia ostreae; Parasitologie.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3366.pdf

Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008
Provedor de dados: 5
Autores: Segarra, Amelie; Pepin, Jean-francois; Arzul, Isabelle; Morga, Benjamin; Faury, Nicole; Renault, Tristan.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 μVar. A microsatellite zone...
Tipo: Text
Palavras-chave: Herpes virus; Oyster; Crassostrea gigas; OsHV-1 mu Var; Increased mortality; Microsatellites.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12260/9035.pdf

Sustainable large‐scale production of European flat oyster (     Ostrea edulis     ) seed for ecological restoration and aquaculture: a review
Provedor de dados: 5
Autores: Colsoul, Bérenger; Boudry, Pierre; Pérez‐parallé, María Luz; Bratoš Cetinić, Ana; Hugh‐jones, Tristan; Arzul, Isabelle; Mérou, Nicolas; Wegner, Karl Mathias; Peter, Corina; Merk, Verena; Pogoda, Bernadette.
The conservation and active restoration of European flat oyster (Ostrea edulis) populations are a major focus of ecological restoration efforts to take advantage of the wide‐ranging ecosystem functions and services this species provides. Accordingly, additional and new demands for seed oysters have arisen. In commercial aquaculture (mariculture), the production of O. edulis is still largely based on natural seed collection. Considering the specific requirements, related to ecological restoration, such as the absence of pathogens and the preservation of high genetic diversity, the current supply is insufficient. Despite the development of breeding and controlled reproduction techniques for this species since the late 1930s, seed production today is mainly...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00678/79001/81394.pdf

Some like it hot: Paracartia grani (Copepoda: Calanoida) arrival in the Thau lagoon (south of France—Mediterranean Sea)
Provedor de dados: 5
Autores: Boyer, Séverine; Arzul, Isabelle; Bonnet, Delphine.
Paracartia grani originates from high latitudes and has progressively been recorded in the Mediterranean Sea since the 1980s. In Thau lagoon, while Acartia clausi and Acartia discaudata present maximum peaks of abundance from January to April and in November and December, both species being perennial over the year, P. grani and Paracartia latisetosa appear in May, develop during summer, in July and August, and slowly decrease in autumn before disappearing from the water column in December. The Acartiidae populations have changed over the years at our monitoring station. Indeed, P. latisetosa was last recorded in August 1983 while P. grani appeared in the lagoon in 1998.
Tipo: Text
Palavras-chave: Paracartia grani; Mediterranean Sea; Long term changes; Acartia spp.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00092/20354/18007.pdf

Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979&amp;#305; in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf

A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Arzul, Isabelle.
Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters,...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Mortality; Ruditapes philippinarum; Manila clam; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-816.pdf

First record of a Marteilia parasite (Paramyxea) in zooplankton populations from a natural estuarine environment
Provedor de dados: 5
Autores: Carrasco, Noelia; Lopez, Immaculada; Alcaraz, M; Furones, M. Dolores; Berthe, Franck; Arzul, Isabelle.
The life cycle of the species forming the genus Marteilia (Phylum Paramyxea), which are marine protozoan parasites of molluscs, is still poorly known, although there is evidence of the need for intermediate hosts. To date, only the calanoid copepod Acartia (Paracartia) grani has been demonstrated to be involved in the life cycle of the parasite infecting flat oysters growing in ponds with a low biodiversity. However, no complex natural environments, such as bays or estuaries, have been studied for a similar purpose. Therefore, a survey for the presence of the protozoan Marteilia was conducted by PCR on the zooplankton community of a natural ecosystem, the Alfacs and Fangar bays in the Delta de l'Ebre (NW Mediterranean). Identification of zooplankton...
Tipo: Text
Palavras-chave: Zooplankton; Harpaticoida sp.; Oithona sp.; Mollusc parasite; Life cycle; Marteilia.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3050.pdf

Bilan 2006 du réseau Repamo. Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Labrut, Sophie; Miossec, Laurence; Arzul, Isabelle; Joly, Jean-pierre; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie.
Created in 1992, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91/67/EEC and 95/70/EC and are a part of the institutional tasks of Ifremer. The aims of the network are to prevent the introduction and spread of exotic  pathogens and to survey the evolution ofnotifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (zone X and Granville bed in zone IX). In 2006, only samples of the Granville bed could be analysed due to the difficulty to obtain flat oysters (sparse oyster...
Tipo: Text
Palavras-chave: Health status; Shellfish; Molluscs; Pathology; Surveillance; Network; État de santé; Coquillages; Mollusques; Pathologie; Surveillance; Réseau.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/rapport-3812.pdf

Bilan 2005 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik.
Created in 1992, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Ils activities are in keeping with two European Directives, 91167/EEC and 95170/EC and are a part of the institutional tasks of Ifremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to sm'vey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Granville bed and zone X). In 2005, only samples of the Granville bed could be analysed due to the difflculty to obtain flat oysters (sparse oyster beds, beds...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00086/19704/17346.pdf

Marteiliosis in mussels: a rare disease?
Provedor de dados: 5
Autores: Gombac, Mitja; Kusar, D.; Ocepek, M.; Pogacnik, Milan; Arzul, Isabelle; Couraleau, Yann; Jenclc, V.
Among 1280 cultured and wild adult Mediterranean mussels, Mytilus galloprovincialis, collected over a 1-year surveillance period from the Slovene Adriatic Sea, 0.3% were histologically positive for the presence of Marteilia spp. The infection was concentrated in winter. Employing the molecular methods of PCR, cloning, DNA restriction and sequencing, only Marteilia refringens type M was detected in all the infected mussels. Although all life-cycle stages of M. refringens severely infected digestive glands, only sporadic disruption of epithelial cells of digestive tubules and focal destruction of digestive tubules were observed in the infected mussels. This was the first detection of M. refringens in M. galloprovincialis from the Slovene Adriatic Sea with...
Tipo: Text
Palavras-chave: Prevalence; Marteilia refringens type M.; Mediterranean mussels Mytilus galloprovincialis; Sequencing; Severe infection; Slovene Adriatic Sea.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00165/27640/26064.pdf

Repamo network : a surveillance tool for mollusc health
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva.
At the present time losses due to diseases are one of main factors limiting the aquaculture development. Unceasing increase of exchanges between different countries favours the risk of introduction and propagation of new pathogen agents. At the beginning of the 90's the European Union laid the surveillance of mollusc health inside each Member State in order to protect the safety of exchanges. Indeed few measures are available to protect molluscs from diseases. Only the prophylactic approach is possible; this approach is based on a zoosanitary surveillance of mollusc populations and/or a development of resistant populations against some diseases. Created in 1986, the French network for surveillance and monitoring of mollusc health (REPAMO) ensures the...
Tipo: Text
Palavras-chave: Marteiliosis; Bonamiosis; Organisation; Mission; REPAMO; Surveillance; Pathogenic agents; Mollusc health.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3315.pdf

Molecular detection and quantification of the protozoan Bonamia ostreae in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle.
Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of &lt;1% and a minimum detection limit of 50 gene copies per reaction. Using...
Tipo: Text
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf

Infection à virus de type herpès chez les larves de coquilles Saint-Jacques
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Renault, Tristan.
King scallop (Pecten maximus) larvae suffered massive mortality during the summer of 2000 at a hatchery located in Brittany. Samples were taken for various types of analyses.
Tipo: Text
Palavras-chave: Pathologie; Pecten maximus; Coquilles St Jacques; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3267.pdf

French scallops: A new host for ostreid herpesvirus-1
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text
Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf

Bilan 2003 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain.
The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf

Observations histologiques et analyses en hybridation in situ de bénitiers Tridacna maxima
Provedor de dados: 5
Autores: Arzul, Isabelle; Lapegue, Sylvie; Renault, Tristan.
Tipo: Text
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00156/26683/81049.pdf

Global invasion genetics of two parasitic copepods infecting marine bivalves
Provedor de dados: 5
Autores: Feis, Marieke E.; Goedknegt, M. Anouk; Arzul, Isabelle; Chenuil, Anne; Den Boon, Onno; Gottschalck, Leo; Kondo, Yusuke; Ohtsuka, Susumu; Shama, Lisa N. S.; Thieltges, David W.; Wegner, K. Mathias; Luttikhuizen, Pieternella C.
Invasive species, and especially invasive parasites, represent excellent models to study ecological and evolutionary mechanisms in the wild. To understand these processes, it is crucial to obtain more knowledge on the native range, invasion routes and invasion history of invasive parasites. We investigated the consecutive invasions of two parasitic copepods (Mytilicola intestinalis and Mytilicola orientalis) by combining an extensive literature survey covering the reported putative native regions and the present-day invaded regions with a global phylogeography of both species. The population genetic analyses based on partial COI sequences revealed significant population differentiation for M. orientalis within the native region in Japan, while introduced...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00514/62569/66922.pdf

Emergence de maladies chez les organismes d'intérêt aquacole : quelques scénarios illustrés d'exemples
Provedor de dados: 5
Autores: Saulnier, Denis; Reynaud, Yann; Arzul, Isabelle; Miossec, Laurence; Le Roux, Frédérique; Goarant, Cyrille.
According to the world organisation for animal health (OIE) an emerging disease is defined as a recently admitted serious illness, whose aetiology can, or not, have already been established, and which is likely to be propagated within a population or between populations, for example at the time of international exchanges of aquatic animals and/or products of aquatic animals. Even though the emerging diseases that affect human health have been much studied, those which affect marine organisms and species of aquaculture interest in particular are poorly documented. By restricting emergence to only infectious diseases, we aim at presenting in a non-exhaustive way some scenarios of the emergence of the diseases of aquacultured species by illustrating them with...
Tipo: Text
Palavras-chave: Cyprinus carpio; Ostrea edulis; Bonamia ostreae; Peneides; Vibrio nigripulchritudo; Herpesvirus; Agent pathogène.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2980.pdf

Essais de transmission de l'infection à virus de type Herpes chez les huîtres creuses, Crassostrea gigas
Provedor de dados: 5
Autores: Arzul, Isabelle.
La récente apparition de nombreuses infections à virus de type herpès chez différentes espèces d'huître nécessite le développement d'outils diagnostiques adaptés. Les techniques de détection utilisées jusqu 'alors pour rechercher ces infections virales, histologie et microscopie électronique à transmission, sont plus ou moins satisfaisantes tant par leur sensibilité, le temps qu'elles nécessitent et leur coût. La PCR appliquée à la détection des herpèsvirus chez les vertébrés est largement documentée (Boyle et Blackwell, 1991 ; Casas et al., 1996; Cassinotti et al. , 1996; de Gee et al., 1996). Par ailleurs, de récents progrès ont été réalisés dans le développement de méthodes moléculaires de détection de virus humains transmissibles par les coquillages...
Tipo: Text
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00391/50210/50837.pdf

Marteilia refringens and Marteilia pararefringens sp. nov. are distinct parasites of bivalves and have different European distributions
Provedor de dados: 5
Autores: Kerr, R.; Ward, G. M.; Stentiford, G. D.; Alfjorden, A.; Mortensen, S.; Bignell, J. P.; Feist, S. W.; Villalba, A.; Carballal, M. J.; Cao, A.; Arzul, Isabelle; Ryder, D.; Bass, D.
Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini, which were synonymized in 2007 and subsequently referred to as M. refringens ‘O-type’ and ‘M-type’. O-type has caused mass mortalities of the flat oyster Ostrea edulis. We used high throughput sequencing and histology to intensively screen flat oysters and mussels (Mytilus edulis) from the UK, Sweden and Norway for infection by both types and to generate multi-gene datasets to clarify their genetic distinctiveness. Mussels from the UK, Norway and Sweden were more frequently polymerase chain reaction (PCR)-positive for M-type (75/849) than oysters (11/542). We did not detect O-type in...
Tipo: Text
Palavras-chave: Marteilia refringens; Marteilia pararefringens; ITS1 rDNA; IGS rDNA; Paramyxida; Ascetosporea; Mytilus edulis; Ostrea edulis.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55566/57177.pdf

VIVALDI, Preventing and mitigating farmed bivalve diseases, HORIZON2020
Provedor de dados: 5
Autores: Arzul, Isabelle; Delangle, Estelle.
The overarching goal of VIVALDI is to increase the sustainability and competitiveness of the European shellfish industry by improving the understanding of bivalve diseases and by developing innovative solutions and tools for the prevention, control and mitigation of the major pathogens affecting the main European farmed shellfish species: Pacific oyster (Crassostrea gigas), mussels (Mytilus edulis and M. galloprovincialis), European flat oyster (Ostrea edulis), clams (Venerupis philipinarum) and scallops (Pecten maximus ). The project addresses the most harmful pathogens affecting either one or more of these shellfish species: the virus OsHV-1, Vibrio species including V. aestuarianus, V. splendidus, V. harveyi and V. tapetis, as well as the parasite...
Tipo: Text
Palavras-chave: AQUACULTURE; BIO ECONOMY; BLUE ECONOMY; HORIZON2020; MOLLUSC DISEASE; SHELLFISH; SUSTAINABLE FARMING.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00694/80566/83775.pdf

Viral gametocytic hypertrophy of Crassostrea gigas in France: from occasional records to disease emergence?
Provedor de dados: 5
Autores: Garcia, Celine; Robert, Maeva; Arzul, Isabelle; Chollet, Bruno; Joly, Jean-pierre; Miossec, Laurence; Comtet, Thierry; Berthe, Franck.
Viral gametocytic hypertrophy was reported for the first time in 2001 in Pacific oyster Crassostrea gigas in France. Since this date, the number of reported cases and the distribution area have increased every year; however, the cases are not associated with macroscopic signs or increased mortality rates. Both male and female gametes were hypertrophied and basophilic inclusions were observed in gamete nuclei, Transmission electron microscopy revealed the presence of viral particles in these intranuclear basophilic inclusions. These particles had characteristics similar to those of the Papillomaviridae and Polyomaviridae families: they were small, non-enveloped, icosahedral, and 44 to 56 nm in diameter, The viral particles were found in male, female and...
Tipo: Text
Palavras-chave: Polyomaviridae; Papillomaviridae; Gonad; Viral garnetocytic hypertrophy; Pacific oyster; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-3288.pdf

First results of an epidemiological study on oyster (Crassostrea gigas) mortality events in France during summer 2008
Provedor de dados: 5
Autores: Miossec, Laurence; Allain, Gwenhael; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Cameron, Angus.
Abnormal mortalities occurred in most oyster producing areas in France during the summer of 2008.  The mortalities were sudden and severe (up to 100%) and mainly affected 6 to 18 month old juveniles.  Only Crassostrea gigas species was affected.  The cause of the mortality was unclear but OsHV-1 virus and bacteria belonging to Vibrionaceae family were frequently detected in affected populations. An epidemiological study was commenced in autumn 2008 in order to identify factors associated with, and the cause (or causes) of these mortalities.  This paper presents the results of the first (descriptive) part of this investigation.  The objectives were to identify the affected population and to describe the pattern of mortalities in time and space.  Mortality...
Tipo: Text
Palavras-chave: France; Crassostrea gigas; Oyster; Mortality; Epidemological study.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6772.pdf

In situ hybridization detection of initial infective stages of Marteilia refringens (Paramyxea) in its host Mytilus galloprovincialis
Provedor de dados: 5
Autores: Carrasco, N; Arzul, Isabelle; Berthe, Franck; Furones, M.
Tipo: Text
Palavras-chave: Mussel; Marteilia refringens; In situ hybridization; Gills; Early stages; Connective tissue.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3941.pdf

Cellular and molecular responses of haemocytes from Ostrea edulis during in vitro infection by the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf

Infection of clams by Perkinsus olseni as an example of the new French Surveillance Programme
Provedor de dados: 5
Autores: Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Francois, Cyrille; Joly, Jean-pierre; Chollet, Bruno; Robert, Maeva; De Blas, Isabelle.
The French network for the surveillance and monitoring of mollusc health (REPAMO) was officially set up in 1992 to control the shellfish status along the French coasts according to the European directives 91/67/EEC and 95/70/EC. The objectives are 1) the surveillance of notifiable diseases present in France (Bonamiosis and Marteiliosis of flat oysters Ostrea edulis), 2) the surveillance of health status of cultured and wild populations of molluscs, 3) the study of abnormal mortality and 4) the control of exchanges from European and third countries. In this context, a new surveillance programme, called Active Targeted Survey, part of the REPAMO, was applied in 2004 in order to improve the monitoring of the health status of the French mollusc populations...
Tipo: Text
Palavras-chave: Surveillance; Bivalvia; Clams; Perkinsus olseni; Pathogen; Pathology.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-6376.pdf

Can the protozoan parasite Bonamia ostreae infect larvae of flat oysters Ostrea edulis?
Provedor de dados: 5
Autores: Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine.
Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled...
Tipo: Text
Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf

REPAMO 2003. Bulletins de la surveillance. Juin à septembre 2003
Provedor de dados: 5
Autores: Arzul, Isabelle.
Ce document compile l’ensemble des bulletins de la surveillance de la santé des mollusques marins émis au cours de l’année 2004 par le réseau REPAMO. Ces bulletins, édités à fréquence mensuelle ou en fonction du nombre d’événements, relatent les événements de mortalité notifiés au réseau ainsi que les résultats des analyses effectuées lors des prélèvements d’animaux réalisés sur les lieux des mortalités. Les bulletins sont diffusés aux instances professionnelles concernées (CRC, CNC, CDPMEM, CNPMEM, centres techniques), à la DGAL, à la DPMA, aux laboratoires d’analyses, à Ifremer ainsi que sur le site internet du REPAMO. Ils ont pour objectif de tenir les  destinataires informés des épisodes de mortalités anormales de mollusques marins rencontrés sur le...
Tipo: Text
Palavras-chave: REPAMO; Surveillance zoosanitaire; Mollusques marins; Mortalités.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00480/59165/61798.pdf

Etude épidémiologique descriptive des mortalités exceptionnelles d'huîtres creuses enregistrées sur le littoral français en 2008
Provedor de dados: 5
Autores: Miossec, Laurence; Allain, Gwenhael; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Cameron, Angus.
En 2008, des mortalités massives d'huîtres creuses (Crassostrea gigas) ont été recensées en France pendant l'été. Ce phénomène, observé de façon récurrente sur les côtés françaises depuis le milieu des années 70, a présenté un caractère exceptionnel en 2008 car il a été observé simultanément dans tous les bassins ostréicoles. De plus, les taux de mortalité calculés ont été particulièrement élevés (80 à 100 % pour de nombreux lots). La détection, fréquente et souvent simultanée, d'herpèsvirus OsHV1 et de plusieurs bactéries de la famille des Vibrionacées dans le cadre de la surveillance menée par le réseau national Repamo (réseau de pathologie des mollusques) a mis en évidence le caractère infectieux de l'épisode épidémique 2008. Une étude épidémiologique a...
Tipo: Text
Palavras-chave: France; Crassostrea gigas; Huîtres; Mortalités; Epidémiologie.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6771.pdf

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae
Provedor de dados: 5
Autores: Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle.
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses....
Tipo: Text
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf

A long term study of bonamiosis in Quiberon bay, France
Provedor de dados: 5
Autores: Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf

Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf

Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy)
Provedor de dados: 5
Autores: Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf