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Pathogenicity of Vibrio penaeicida or white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor
Provedor de dados: 5
Autores: Aguirre Guzman, G; Ascencio, F; Saulnier, Denis.
The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p &lt; 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to...
Tipo: Text
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus vannamei; Cysteine protease; Vibrio penaeicida.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3618.pdf