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Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
Provedor de dados: 96
Autores: Machado,Gilmara Junqueira; Murgas,Luis David Solis; Palhares,Priscila Cotta; Assis,Isadora de Lima; Castro,Tássia Flávia Dias.
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Biotechnology; Cryopreservation; Eggs; Fish; Larvae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155