Consulta: creatorFacets:"Atmar, Robert"
Registros recuperados: 5
Data/hora: 13/06/2024 04:39:16

Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of "Norwalk-like" viruses and hepatitis A virus in stool and shellfish
Provedor de dados: 5
Autores: Schwab, Kellogg; Neill, Frederick; Le Guyader, Soizick; Estes, Mary; Atmar, Robert.
Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by ne,ver diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalklike" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV detection from environmental samples such as shellfish, we have developed an RT-PCR-oligoprobe amplification and detection method using several new procedures that enable confirmed RT-PCR amplification and product detection in 1 day. The new steps include replacing reverse transcriptase and Tag polymerase with rTth polymerase, a heat-stable enzyme that functions as...
Tipo: Text
Palavras-chave: Bivalvia; Immuoessay; DNA enzyme; Reverse transcription PCR; Detection  method; Enteric virus.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-1267.pdf

Norovirus contamination on French marketed oysters
Provedor de dados: 5
Autores: Schaeffer, Julien; Le Saux, Jean-claude; Lora, Monica; Atmar, Robert; Le Guyader, Soizick.
Contaminaded shellfish have been implicated in gastroenteritis outbreaks in different countries. As no regulation has been set up yet regarding viral contamination of food, very few data are available on the prevalence of contamined products on the market. This study presents data obtained from oysters collected on the French market in one producing area over a 16 month period of time. Noroviruses were detected in 9% of samples with a seasonal impact and influence of climatic events. Contamination levels were low and, surprisingly, oysters sampled directly from the producer were found to have less contamination than oystes from supermarkets.
Tipo: Text
Palavras-chave: Oyster; Marker; Norovirus; Quantification; Calicivirus; Prevalence.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26367/24457.pdf

A scientific review of binding and inactivation of viruses on and in food, with a focus on the role of the matrix
Provedor de dados: 5
Autores: Le Guyader, Soizick; Atmar, Robert.
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00066/17762/15276.pdf

Shellfish contamination by norovirus : strain selection based on ligand expression?
Provedor de dados: 5
Autores: Le Guyader, Soizick; Atmar, Robert; Maalouf, Haifa; Le Pendu, Jacques.
Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of NoV GI strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to...
Tipo: Text
Palavras-chave: Shellfish; Noroviruses; Oyster contamination; Pathogen.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00150/26093/24313.pdf

Monitoring viral contamination in shellfish growing areas
Provedor de dados: 5
Autores: Le Guyader, Soizick; Pommepuy, Monique; Atmar, Robert.
Human and animal fecal wastes and urine contain a large number of different viruses that can enter the environment through the discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. Viruses are present in shellfish in very low numbers. Nevertheless, they are present in sufficient quantities to pose a health risk as presented. This low level of contamination has made it necessary to develop highly sensitive viral extraction methods to ensure virus recovery from shellfish tissues. The most common route for accidental contamination is after heavy...
Tipo: Text
Palavras-chave: Human enteric viruses; Shellfish; Sewage; Persistence; Flux.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00015/12606/15279.pdf