Consulta: creatorFacets:"Azevedo,J.L."
Registros recuperados: 5
Data/hora: 05/06/2024 05:41:23

Selection for breed-specific growth hormone and IGF-I alleles in a synthetic beef cattle cross, Canchim
Provedor de dados: 74
Autores: Regitano,L.C.A.; Azevedo,J.L.; Vencovsky,R.; Packer,I.U.; Barbosa,P.F.; Rosa,A.J.M.; Silva,N.A.; Etchegaray,M.A.L.; Coutinho,L.L.
This study was developed to evaluate selection effects on gene frequencies in a synthetic beef cattle cross (5/8 Charolais, 3/8 Zebu) named Canchim. A sample of 154 Canchim animals, representing three generation classes, was analyzed for seven molecular markers. Thirty-six Charolais cattle were also studied for comparison. A highly significant variation (P &amp;lt; 0.01), with a linear increase of the allele coding for valine at position 127 of the growth hormone peptide, was observed through generations. This allele was found in the Charolais sample but is not observed in Brazilian Bos indicus breeds. Four alleles were found for a microsatellite in the 5' flanking region of insulin-like growth factor I gene in Canchim. Allele sizes ranged from 231 to 225...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400011

Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
Provedor de dados: 63
Autores: Kava - Cordeiro,V.; Luna - Alves - Lima,E.A.; Azevedo,J.L.
A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium anisopliae; Auxotrophk mutants; Colour mutants; Mutagenic agents; Gamma radiation; Ultraviolet light; Nitrous acid.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023

Eliminação da produção de bacteriocinas em Erwinia e Pseudomonas fitopatogênicas
Provedor de dados: 63
Autores: Biagi,C.M.R. de; Azevedo,J.L.
Duas linhagens de bactérias fitopatogênicas do gênero Pseudomonas e duas do gênero Erwinia produtoras de bacteriocinas, foram submetidas a diferentes tratamentos visando estimar a estabilidade desse caráter. Houve eliminação da produção de bacteriocinas após tratamento com duas concentrações de brometo de etídio e a porcentagem de eliminação variou com o hospedeiro e com a concentração da droga. Não houve eliminação em temperaturas elevadas e também a preservação da linhagem por três anos em laboratório não causou perda do caráter. A eliminação da produção de bacteriocinas com brometo de etídio sugere que os genes envolvidos tenham localização plasmidiana.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriocina; Erwinia; Pseudomonas.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000100024

A biolistic process for in vitro gene transfer into chicken embryos
Provedor de dados: 56
Autores: Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L.
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003

Fungicide resistance and genetic variability in plant pathogenic strains of Guignardia citricarpa
Provedor de dados: 58
Autores: Possiede,Y.M.; Gabardo,J.; Kava-Cordeiro,V.; Galli-Terasawa,L.V.; Azevedo,J.L.; Glienke,C.
Citrus black spot (CBS) is a plant disease of worldwide occurrence, affecting crops in Africa, Oceania, and South America. In Brazil, climate provides favorable conditions and CBS has spread to the Southeast and South regions. CBS is caused by the fungus Guignardia citricarpa (anamorph: Phyllosticta citricarpa) and its control is based on the use of fungicides, such as benzimidazoles. In South Africa, the disease was kept under control for 10 years with benomyl, until cases of resistance to high concentrations of this fungicide were reported from all citrus-producing areas. Azoxystrobin (a strobilurin) has been found effective in controlling phytopathogens, including CBS, in a wide range of economically important crops. The present study investigated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: G. citricarpa; Azoxystrobin; Benomyl; Citrus black spot; Fungicides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200018