Consulta: creatorFacets:"Barbosa,Graziela da Silva"
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Data/hora: 09/06/2024 13:54:39

A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses
Provedor de dados: 124
Autores: Nascimento,Aline Kelly Queiróz; Lima,José Albersio Araújo; Barbosa,Graziela da Silva.
ABSTRACT Many methods can be used for arriving in a correct virus disease diagnose, and the serological techniques are the most used methods for plant virus identification. The indirect enzyme-linked immunosorbent assay (Indirect-ELISA) or plate-trapped antigen ELISA (PTA-ELISA) has been useful for detection of viruses in a wide range of situations, especially to test a large number of samples in a relatively short period of time. Immune-biological Companies have developed practical kits for direct ELISA or double antibody sandwich (DAS-ELISA), but neither Company has developed kits for PTA-ELISA. As a single universal antibody-conjugate is used for detection of a wide range of plant viruses, the PTA-ELISA technique is more economical, practical and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant Virus Diagnose; ELISA; Comovirus; Cucumovirus; Potyvirus; Sobemovirus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100216

Sources of resistance in accessions of Cucurbita spp. to virus species from the genus Potyvirus
Provedor de dados: 124
Autores: Barbosa,Graziela da Silva; Lima,José Albersio Araújo; Nascimento,Aline Kelly Queiróz do; Silva,Fabiana Rodrigues; Dias,Rita de Cássia Souza.
ABSTRACT The identification of source of resistance in cultivated cucurbits species is very important for the development of resistant cultivars to control diseases caused by virus from the genus Potyvirus. The present research had the objective to evaluate the phenotypic reactions and the behavior of pumpkin (Cucurbita spp.) accessions to the virus species from the genus Potyvirus: Papaya ringspot virus type Watermelon (PRSV-W), Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Twenty-eight accessions of pumpkin from the Cucurbit Germplasm Bank from Embrapa Semiárido, Petrolina, PE, Brazil were evaluated. Twelve young plants from each pumpkin accession were inoculated with each one of the virus species and were maintained at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PRSV-W; WMV; ZYMV; Cucurbits; Plant resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000400725