Consulta: creatorFacets:"Bardouil, Michele"
Registros recuperados: 40
Data/hora: 07/06/2024 04:34:46

Paralytic shellfish poison accumulation yields and feeding time activity in the Pacific oyster (Crassostrea gigas) and king scallop (Pecten maximus)
Provedor de dados: 5
Autores: Bougrier, Serge; Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Truquet, Philippe.
Pacific oysters and king scallops placed individually in a recirculating flume were fed for 2 weeks with a constant concentration (120 cell ml(-1)) of a toxic strain of Alexandrium minutum. Fluorescence at the outlet of each experimental unit was measured continuously, and biodeposits were recovered twice daily to evaluate feeding time activity (FTA) and rates of organic filtration (OFR), ingestion (OIR) and organic absorption (OAR). Ion-pairing high performance liquid chromatography (IP-HPLC) was performed concurrently to quantify paralytic phycotoxins both (i) individually in shellfish at the end of the contamination period and (ii) in A. minutum cultures to estimate cellular toxin concentration. These data allowed comparison of the actual tissue toxin...
Tipo: Text
Palavras-chave: Alexandrium minutum; Crassostrea gigas; Feeding activity; Paralytic shellfish poisoning.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-576.pdf

Paralytic phycotoxin uptake by scallops (Pecten maximus)
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele; Ledoux, Martial; Murail, Ingrid; Bohec, Madeleine; Truquet, Philippe; Frémy, Jean-marc; Rohmer, Véronique.
It is difficult to apply sanitary standards for mollusc contamination by paralytic shellfish poisoning to animals not consumed whole. This problem is illustrated by the 1990 embargo on a Japanese shipment of frozen scallop muscle and gonad: the sanitary threshold applied to digestive gland (in Japan) does not guarantee that muscle and especially gonad are toxin-free. Accordingly, we performed experimental contaminations of scallops (Pecten maximus) from Port-en-Bessin (Normandy, France) using a toxic Japanese strain of Alexandrium tamarense. During the contamination/decontamination experiment on different tissues (digestive gland, muscle, gonad), extracts were obtained using cold acetic acid 0.1 N to maintain the perfect integrity of the toxin profile....
Tipo: Text
Palavras-chave: Alexandrium tamarense; Phycotoxines; Bioaccumulation; Profils toxiniques; Pecten maximus; Patinopecten yessoensis; Alexandrium tamarense; Phycotoxins; Bioaccumulation; Toxin profiles; Patinopecten yessoensis; Pecten maximus.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00189/30075/28563.pdf

Modélisation de la contamination des bivalves par les toxines d'Alexandrium spp. Rapport final
Provedor de dados: 5
Autores: Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie.
L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs.
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf

Le maximum saisonnier de Dynophysis cf acuminata à Antifer (Normandie, France)
Provedor de dados: 5
Autores: Lassus, Patrick; Maggi, Pierre; Proniewski, F.; Truquet, Philippe; Bardouil, Michele.
Since 1987 Antifer oil tanker terminal (Normandy, France) was weIl known, as a preferential area for summer highest blooms of Dinophysis cf acuminata, a toxic dinoflagellate. Three oceanographic cruises were made in August 1989, before, in the course of and after the seasonal max. of this species. Results obtained from these cruises (hydrological parameters and phytoplankton) as weIl as from a daily survey at a reference station in Antifer harbour, corrobora te the prevailing role of coastal up flowing of Seine estuarine waters, pushed to the coast by a dominant S - SW wind, on intensity and duration of Dinophysis bloom. Such observations are in agreement with those of Summer 1987 at reference station.
Tipo: Text
Palavras-chave: Antifer; Dinophysis; Hydrologie; Phytoplancton; Antifer; Dinophysis; Hydrology; Phytoplankton.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00138/24946/23039.pdf

Cinétiques de décontamination en milieu contrôlé de moules toxiques (Diarrheic Shellfish Poison). Premiers résultats
Provedor de dados: 5
Autores: Bardouil, Michele; Masselin, Pierre; Bohec, Madeleine.
Laboratory assays have been performed, using mussels contamined with Dinophysis, in order to assess potential depurations kinetics. Mussels samples from Seine bay, Douarnenez bay and Vilaine bay have been tested . From these assays, which are to be performed in 1989; two main results are stressed : 1) when the toxicity level is near 0,5 MU/g of hepatopancreas*, depuration is undedectable during twelve days, 2) when the toxicity level exceeds 1 MU/g,depuration is important during the first eight days. *(quarantine level used by Japanese authorities) .
Tipo: Text
Palavras-chave: Moules; DSP; Dinophysis; Décontamination; Blue mussels; DSP; Dinophysis; Depuration.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00430/54148/55479.pdf

Etude de la décontamination de moules toxiques (toxines diarrhéiques) en laboratoire et en milieu naturel
Provedor de dados: 5
Autores: Marcaillou-le Baut, Claire; Bardin, B.; Bardouil, Michele; Bohec, Madeleine; Le Dean, Loic; Masselin, Pierre; Truquet, Philippe.
Two studies of depuration, one in laboratory conditions and the other one in an oyster's pond were performed using 2 sets of contaminated mussels with diarrhetic toxins at a high and a low level (3 and 1 M.U.). Depuration kinetics were followed both by the adult mouse test and okadaïc acid analysis. Results are - in the pond, high toxic mussels need about 20 days to become eatable (toxicity level : 0.5 M.U.g-1 digestive glands), -this time decreases when the initial toxicity is low but it increases when mussels are underfed, - a good correlation is observed between the total toxicity and okadaïc acid concentration.
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00075/18595/16148.pdf

Improving detoxification Efficiency of PSP-contaminated oysters (Crassostrea gigas Thunberg)
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp.
Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large...
Tipo: Text
Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf

Juin 1986 : Distribution de Dinophysis au large de la Bretagne sud
Provedor de dados: 5
Autores: Lassus, Patrick; Le Dean, Loic; Bocquene, Gilles; Bardouil, Michele; Truquet, Philippe; Truquet, Isabelle; Larrazabal, M.e.
During EIGAS échointegration cruise, hydrological and phytoplanktonic sampling operations have been carried out along off shore and coastal South Brittany area, from June 8 th to 20 - 1986. Dinophysis cells densities have been evaluated at 3 depths and for 32 statlons between Penmarc'h and Belle Ile, and positive correlation with water stability index have been pointed out; high cells densities area have been detected in the South of Loire estuary
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00119/23000/20830.pdf

Gut passage times in two bivalve molluscs fed toxic microalgae: Alexandrium minutum, A-catenella and Pseudo-nitzschia calliantha
Provedor de dados: 5
Autores: Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent.
The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (...
Tipo: Text
Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf

Paralytic shellfish poison outbreaks in the Penze estuary: Environmental factors affecting toxin uptake in the oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Lassus, Patrick; Baron, Regis; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Leguay, Didier; Amzil, Zouher.
Several experiments using a self-regulated system were conducted to define the factors likely to influence the uptake of paralytic shellfish poison (PSP) by oysters in the Penze estuary (France, Brittany). Each 4-day experiment was carried out in a recirculated sea water system using 15 Pacific oysters (Crassostrea gigas) separated from each other and supplied with unfiltered natural seawater containing alternatively toxic (Alexandrium minutum) or non-toxic (Skeletonema costatum) algal diets. The food supply and exposure times to toxic diets were determined according to field studies of the Upstream and downstream movement of patches containing A. minutum. The experimental parameters corresponded roughly to the hydrological conditions generally observed in...
Tipo: Text
Palavras-chave: Experimental contamination; Oyster toxicity; Dinophyceae; Toxin; PSP.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-400.pdf

Modélisation de la contamination des huîtres par les toxines d'Alexandrium minutum - Rapport final (2ème année)
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille.
L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer).
Tipo: Text
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf

Effect of the PSP-causing dinoflagellate, Alexandrium sp. on the initial feeding response of Crassostrea gigas
Provedor de dados: 5
Autores: Wildish, David; Lassus, Patrick; Martin, Jennifer; Saulnier, Alinne; Bardouil, Michele.
The effects of toxic and non-toxic strains of Alexandrium sp. on the initial feeding responses of the Pacific oyster Crassostrea gigas were determined by continuous monitoring of the clearance rate of individual oysters. This was determined fluorometrically by loss of fluorescence of live microalgae introduced as food. Feeding oysters with toxic or non-toxic A. tamarense and toxic A. fundyense resulted in stop/start clearance behaviour. In contrast, control oysters fed a reference microalga, Isochrysis sp., known to support their growth, did not exhibit this behavior. Pacific oysters acclimated to Isochrysis sp., fed mixtures of Alexandrium/Isochrysis, showed further evidence of stop/start clearance. Exposure for periods up to 48 hours with non-toxic A....
Tipo: Text
Palavras-chave: Crassostrea gigas; Physiologie alimentaire; Microalgues toxiques; Dinoflagellés; Paralytic shellfish poisoning; Crassostrea gigas; Feeding physiology; Toxic microalgae; Dinoflagellate; Paralytic shellfish poisoning.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-877.pdf

Influence des rejets de l'usine "Thann et Mulhouse" sur les organismes marins - Mesures de pH "in situ" et relation avec les tests d'écotoxicité
Provedor de dados: 5
Autores: Proniewsk, F.; Lassus, Patrick; Bocquene, Gilles; Miossec, Laurence; Bardouil, Michele.
Dans deux rapports précédents (LASSUS et PRONIEWSKI, 1982 - LE DEAN et Al. , 1983) un certain nombre d'éléments de réponse avaient été apportés quant aux effets d'un pH bas (2,8 à 3,0) en milieu marin, pendant une durée brève, sur la faune et la flore marines.
Tipo: Text
Ano: 1984
URL: http://archimer.ifremer.fr/doc/00045/15632/13035.pdf

Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution
Provedor de dados: 5
Autores: Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele.
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of...
Tipo: Text
Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf

The diurnal vertical migrations of Dinophysis acuminata in an outdoor tank at Antifer (Normandy, France)
Provedor de dados: 5
Autores: Lassus, Patrick; Proniewski, Frédéric; Pigeon, Cyrille; Veret, Laurent; Le Déan, Loic; Bardouil, Michele; Truquet, Philippe.
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00189/30070/28558.pdf

Modelling of paralytic shellfish toxin biotransformations in the course of Crassostrea gigas detoxification kinetics
Provedor de dados: 5
Autores: Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Truquet, Philippe; Haberkorn, Hansy; Lassus, Patrick; Barille, Laurent; Amzil, Zouher.
The aim of this study was to evaluate the importance of biotransformation of paralytic shellfish toxins during the detoxification process in contaminated oysters. Mathematical models based upon the detoxification patterns of digestive gland and other tissues were developed. It was demonstrated that biotransformations do not seem to play an important role in digestive gland or other tissue detoxification kinetics with our data set. Moreover, different toxin transfers from digestive gland toward other tissues were investigated. No significant transfer was highlighted in our data set. These first conclusions were drawn after comparing the results obtained from 13 biotransformations and identifiable transfer scenarios. Finally, to determine a more robust...
Tipo: Text
Palavras-chave: Detoxification kinetics modelling; Biotransformation of saxitoxin analogues; Oyster.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00049/16047/14309.pdf

Continuous fluorescence recording as a way to improve Pacific oyster (Crassostrea gigas) models of paralytic shellfish toxin accumulation
Provedor de dados: 5
Autores: Baron, Regis; Couedel, Marielle; Joret, Camille; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Lassus, Patrick.
A simple system was used to simulate the effect of alternating toxic (paralytic shellfish poisoning toxins) and non-toxic microalgal diets on oyster feeding behaviors and rates of toxin accumulation. These experimental conditions were meant to reflect, to some extent, the incoming and outgoing fluxes of toxic algae observed at the mouth of the Penze estuary (Northern Brittany, France). Physiological and toxicological parameters were estimated based oil fluorescence measurements recorded continuously at the outlet of each experimental tank, which contained a single oyster. Q(tox), this variable describes toxin uptake in oysters, it was used (instead of the toxin ingestion rate): i) in simple graphical analyses, ii) as well as in one- and two-compartment...
Tipo: Text
Palavras-chave: Skeletonema costatum; Alexandrium minutum; Fluorescence; Modeling; Kinetics; Toxin uptake; Shellfish toxin; PSP.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1400.pdf

Distribution de Dinophysis cf acuminata, et des espèces associées en baie de Vilaine, pendant l'été 1985
Provedor de dados: 5
Autores: Lassus, Patrick; Maggi, Pierre; Truquet, Isabelle; Truquet, Philippe; Bardouil, Michele; Larrazabal, M.e.
Dinophysis cf. acuminata concentrations have been monitored from April to July 1985 in Vilaine Bay with 20 sampling stations and at several depths. Besides, variations in biomasses for other species have been analysed against Temperature/Salinity patterns. This study confirms 1) The importance of stratification and coastal currents in Dinophysis distribution, 2) the influence of bottom morphology on cumulative phenomenons as observed in the inner part of the Bay. In the same way, waters stratification would play an important role in species succession.
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00119/22999/20829.pdf

Influence of Crassostrea gigas (Thunberg) sexual maturation stage and ploidy on uptake of paralytic phycotoxins
Provedor de dados: 5
Autores: Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Haberkorn, Hansy; Soudant, Philippe; Truquet, Philippe; Lassus, Patrick.
The purpose of this study was to assess paralytic phycotoxin uptake in diploid and triploid oysters at two stages of their sexual cycle corresponding to their status in early summer (June) and winter (November). Samples of diploid and triploid oysters were exposed to a toxic culture of Alexandrium minutum for 4 days in each season. No significant differences in filtration or clearance rates were observed during either November or June experiments. When diploid oysters were at resting stage (November), toxin uptake showed no significant difference between the ploidy classes. In contrast, when the diploid oysters were at the peak of their sexual maturation (June), the triploid oysters were seen to accumulate almost double the amount of paralytic toxins as...
Tipo: Text
Palavras-chave: Paralytic shellfish poisoning; Crassostrea gigas; Diploid; Triploid; Contamination.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19796/17487.pdf

Pinnatoxines en lien avec l’espèce Vulcanodinium rugosum (II)
Provedor de dados: 5
Autores: Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi.
Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés.....
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf

Décontamination expérimentale de bivalves toxiques : application aux phycotoxines paralysantes. Rapport final
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Naviner, Magali; Truquet, Philippe.
A sea water recirculating flume was especially designed in the framework of this study. It improves physiological and ecophysiological evaluation of a 25 oysters (c. gigas) set fed on PSP toxic A. minutum cultures and then detoxified with commonly used aquaculture species (I. galbana, T. suecica, S. costatum, T. weissflogii). Chlorophyll a levels are kept steady in each case and adjusted in order to reach the same food value in each tank (0,5 mg/l TPM). When exposed to the mildly toxic species A. minutum (0,5 pg.eq.STX.cel-1) oysters depict a reduced clearance rate, food uptake and shell-valves activity (compared to non-toxic algal diets). The same physiological parameters do not show any significant differences when oysters are exposed to the control al...
Tipo: Text
Palavras-chave: Épuration; Crassostrea gigas; Alexandrium minutum; PSP; Écophysiologie; Depuration; C. gigas; A. minutum; PSP; Ecophysiology.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00139/25021/23126.pdf

ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton
Provedor de dados: 5
Autores: Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...
Tipo: Text
Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf

Présence d'un Protogonyaulax sp. sur le littoral atlantique français pendant l'hiver 1987
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele.
A dinoflagellate belonging to Protogonyaulax genera and forming more than 20 cells chains was observed from November 1987 to February 1988 in Vilaine Bay and in the west ofIle d'Yeu (France). This species was not detected in coastal phytoplankton during preceding surveys. Close to Protogonyaulax affinis it seems to be a winter developing species for this coastal area. Vertical distribution shows in december a migration of the patch towards deeper layers. Increasing occurrences of species belonging to Protogonyaulax (Alexandrium Balech) genera inshore or coastal waters need to pay more attention to the taxonomy of such organisms. Some species are effectively well known as poisons producers.
Tipo: Text
Palavras-chave: Pyrrophycophyta; Milieu marin; Morbihan; Vendée; Océan Atlantique Nord; Plancton; Morphologie; Algae; Thallophyta; Bretagne; France; Europe; Pays de Loire; Océan Atlantique; Pyrrophycophyta; Marine environment; Morbihan; Vendée; North Atlantic; Plankton; Morphology; Algae; Thallophyta; Bretagne; France; Europe; Pays de Loire; Atlantic Ocean.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00133/24474/22485.pdf

Distribution du dinoflagellé Dinophysis sur les côtes françaises - Données taxonomiques récentes
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele.
A partir d'observations récentes en microscopie photonique et à balayage il est devenu nécessaire de réviser la dénomination de Dinophysis acuminata donnée à différentes espèces proches. Des échantillons phytoplanctoniques prélevés en différents points des côtes françaises ont été examinés par le Professeur BALECH en Argentine et les exemplaires de Méditerranée et des côtes sud bretonnes ont été identifiés comme D. sacculus. Compte tenu des données bibliographiques sur la répartition du genre Dinophysis sur les côtes françaises et du caractère toxique de certaines espèces, quelques recommandations sont émises concernant les dénombrements effectués dans le cadre du contrôle phytoplanctonique des eaux conchylicoles.
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00119/23001/20831.pdf

Juin 1987 - Distribution de Dinophysis au large de la Bretagne sud (II)
Provedor de dados: 5
Autores: Lassus, Patrick; Truquet, Philippe; Le Dean, Loic; Bardouil, Michele; Truquet, Isabelle.
As a part of hydrological sampLing operation in South Brittany, 8 transects for 26 sampling stations were made in order to determine horizontal, and vertical distribution of Dinophysis sacculus in the 10 m layer. Un like to 1986, results do not show any correlations between Dinophysis concentrations and stability index. That may, nevertheless, be due to lower cells densities in 1987 when compared to 1986. A thermal, gradient, lower than in 1986, is still observable in the Bouthern of Loire Mouth. And, as a last point, when chlorophyll a concentrations are higher in - 10 m layer than in surface layer (Audierne and Noirmoutier areas), it can be observed hight densities of an other toxie Dinoflagellate : Gyrodinium aureolum .
Tipo: Text
Palavras-chave: Bretagne sud; Hydrologie; D. sacculus; G. aureolum; South Brittany; Hydrology; D. sacculus; G. aureolum.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00138/24921/23015.pdf

Alexandrium ostenfeldii growth and spirolide production in batch culture and photobioreactor
Provedor de dados: 5
Autores: Medhioub, Walid; Sechet, Veronique; Truquet, Philippe; Bardouil, Michele; Amzil, Zouher; Lassus, Patrick; Soudant, Philippe.
Growth and spirolide production of the toxic dinoflagellate Alexandrium ostenfeldii (Danish strain CCMP1773) were studied in batch culture and a photobioreactor (continuous cultures). First, batch cultures were grown in 450 mL flasks without aeration and under varying conditions of temperature (16 and 22 degrees C) and culture medium (L1, f/2 and L1 with addition of soil extract). Second, cultures were grown at 16 degrees C in 8 L aerated flat-bottomed vessels using L1 with soil extract as culture medium. Finally, continuous cultures in a photobioreactor were conducted at 18 degrees C in L1 with soil extract; pH was maintained at 8.5 and continuous stirring was applied. This study showed that A. ostenfeldii growth was significantly affected by temperature....
Tipo: Text
Palavras-chave: Alexandrium ostenfeldii; Batch; Growth; Photobioreactor; Spirolide production.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00049/16050/15784.pdf

Le complexe «Dinophysis acuminata»: identification des espèces le long des côtes françaises
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele.
From seawater samples collected between 1984 and 1989 in 12 French coastal areas, a morphometric study have been realised on Dinophysis genera restricted to "acuminata" like species. Simple morphological criterias are given as suitable for discrimination of four dominant species occurring during toxic episodes in shellfish farms (Diarrheic Shellfish Poisoning): Dinophysis c-closed-integral. sacculus, D. c-closed-integral. acuminata, Dinophysis sp. and D. c-closed-integral. norvegica. This last species cannot be included in the "acuminata complex" and produces temporary blooms along southern Brittany coasts. Pending a more precise diagnosis it is recommended to clarify the relationship between toxin composition and each species previously described.
Tipo: Text
Palavras-chave: Europe; Thallophyta; Algae; Répartition géographique; Systématique; Morphométrie; France; Zone littorale; Identification spécifique; Pyrrophycophyta; Répartition géographique; Systématique; Morphométrie; France; Zone littorale; Identification spécifique; Pyrrophycophyta; Dinophysis; Répartition géographique; Systématique; Europe; Thallophyta; Algae; Geographic distribution; Taxonomy; Morphometry; France; Littoral zone; Specific identification; Pyrrophycophyta; Geographic distribution; Taxonomy; Morphometry; France; Littoral zone; Specific identification; Pyrrophycophyta; Geographic distribution; Taxonomy; Morphometry.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00133/24471/22486.pdf

Seasonal occurrence of Dinophysis sp. along the French coast between 1983 and 1987
Provedor de dados: 5
Autores: Lassus, Patrick; Bardouil, Michele; Berthomé, Jean-paul; Maggi, Pierre; Truquet, Philippe; Le Déan, Loic.
On the basis of hydrological and biological data collected between 1983 and 1987 along the French coast, and especially in the southern approaches to Brittany. it has been possible to compare different parameters in an attempt to explain the seasonal variations of a toxic dinoflagellate: Dinophysis sp. speveral species were identified and their seasonal distribution patterns are described. The origin of the blooms, from either coastal endemic or offshore populations, is considered
Tipo: Text
Palavras-chave: Dinophysis; Distribution saisonnière; Côtes françaises; Dinophysis; Seasonal distribution; French coast.
Ano: 1988
URL: https://archimer.ifremer.fr/doc/00189/30067/28555.pdf

Influence d'un traitement au chlore sur la croissance d'une diatomée : Phaeodactylum Tricornutum (BOHLIN)
Provedor de dados: 5
Autores: Bardouil, Michele; Bocquene, Gilles; Le Dean, Loic.
Ces travaux complètent l'étude de l'influence de chocs thermiques sur la croissance de la diatomée Phaeodactylum tricornutum (BOHLIN). Ils concernent les effets d'un traitement au chlore sur le développement de cette diatomée.
Tipo: Text
Ano: 1981
URL: http://archimer.ifremer.fr/doc/00046/15749/13151.pdf

Kystes modernes de Dinoflagellés en Baie de Vilaine-Bretagne Sud (France)
Provedor de dados: 5
Autores: Larrazabal, Me; Lassus, Patrick; Maggi, Pierre; Bardouil, Michele.
A preliminary study of modern marine dinoflagellate cysts has been performed in Vilaine Bay through winters 1986, 1987 and 1989. Direct observations of muddy sand samples and most probable number (MPN) method applied to cysts have evidenced a low win ter species diversity with Spiniferites spp. and Scrippsiella sp. cysts as predominant species. Change in relative importance of maximum species diversity area from ycar to year suggests a possible action of bottom currents. A study of dormant stages of toxie dinoflagellates, genus Alexandrium and Dinophysis, has corroborated the occurrence of A. minutum cysts (IO to 30 cysts.g-I sediment) at low amounts and absence of Dinophysis sp. resting stages. More generally, comparison of cysts in sediments with summer...
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00133/24472/22488.pdf

Résultats des observation réalisées en 1981 sur le site de la centrale thermique de Cordemais - Effets des ouvrages de prise et de rejet sur l'ichthyofaune locale
Provedor de dados: 5
Autores: Lassus, Patrick; Le Baut, Claire; Bardouil, Michele; Bocquene, Gilles; Le Dean, Loic; Truquet, Philippe.
A la suite des dossiers d'impact réalisés sur la centrale de Cordemais il est apparu rapidement que les autorités intéressés {EDF (1), MECV (2), Port Autonome} manquaient d'information sur l'influence des ouvrages de prise d'eau vis-à-vis de la faune estuarienne.
Tipo: Text
Ano: 1982
URL: https://archimer.ifremer.fr/doc/00045/15625/13028.pdf

Detoxification enhancement in the gymnodimine-contaminated grooved carpet shell, Ruditapes decussatus (Linne)
Provedor de dados: 5
Autores: Medhioub, Walid; Gueguen, Marielle; Lassus, Patrick; Bardouil, Michele; Truquet, Philippe; Sibat, Manoella; Nejib, Medhioub; Philippe, Soudant; Mejdeddine, Kraiem; Amzil, Zouher.
In the Gulf of Gabes (Tunisia, Eastern Mediterranean sea), the grooved carpet shell Ruditapes decussatus has been seen to contain persistent levels of gymnodimine (GYM) for several years. The present experimental work represents the first attempt to assess detoxification kinetics of fast-acting toxins (FAT) in marine molluscs fed specific diets of non-toxic algae (Isochrysis galbana). To find an optimal detoxification method, two experiments were performed in which clams were first fed the toxic dinoflagellate Karenia selliformis to artificially contaminate them with GYM, thus simulating the effect of natural toxic episodes. As a second step, the same clams were fed a non-toxic algae, I. galbana. to speed up the detoxification process. Changes in toxin...
Tipo: Text
Palavras-chave: Ruditapes decussates; Grooved carpet shell; Karenia selliformis; Gymnodimine; Toxicity; Detoxification.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2010/publication-7488.pdf

Feeding responses of Crassostrea gigas (Thunberg) to inclusion of different proportions of toxic dinoflagellates in their diet
Provedor de dados: 5
Autores: Bardouil, Michele; Bohec, Madeleine; Bougrier, Serge; Lassus, Patrick; Truquet, Philippe.
An experimental flow-through system allowing determination of the feeding behaviour of individual molluscs was used to study oysters exposed to mixed diets composed of varying proportions of the diatom Thalassiosira weissflogii and two strains (toxic and nontoxic) of the dinoflagellate Alexandrium larnarense. Our results show that, when compared to a T. weissflogii unialgal diet, even a diatomltoxic dinoflagellate ratio as low in biomass as 90/10 reduced clearance rates and biodeposit production by oysters. Consumption was slightly but significantly decreased for a 50/50 diatomltoxic dinoflagellate mixture. For the toxic dinoflagellate unialgal diet, ingestion, absorption and consumption were completely inhibited. Thus, the inclusion of low amounts of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiologie; Toxine paralysante des coquillages; Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiology; Paralytic shellfish poison.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00017/12788/9729.pdf

Influence of environmental parameters on Karenia selliformis toxin content in culture
Provedor de dados: 5
Autores: Medhioub, Amel; Medhioub, Walid; Amzil, Zouher; Sibat, Manoella; Bardouil, Michele; Ben Neila, Idriss; Mezghani, Salah; Hamza, Asma; Lassus, Patrick.
Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabes gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae oil enriched Gaillard f/2 medium. The influence of growing conditions oil toxin content was studied, examining the effects of (i) different culture Volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15 degrees C et 20-21 degrees C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 +/- 0.14 div d(-1)) was obtained at 20 degrees C...
Tipo: Text
Palavras-chave: Mouse bioassay; Gymnodimine; Toxicity; Growth; Karenia selliformis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7383.pdf

Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon
Provedor de dados: 5
Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher.
beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral...
Tipo: Text
Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf

Modelling the accumulation of PSP toxins in Thau Lagoon oysters (Crassostrea gigas) from trials using mixed cultures of Alexandrium catenella and Thalassiosira weissflogii
Provedor de dados: 5
Autores: Lassus, Patrick; Amzil, Zouher; Baron, Regis; Sechet, Veronique; Barille, Laurent; Abadie, Eric; Bardouil, Michele; Sibat, Manoella; Truquet, Philippe; Berard, Jean-baptiste; Gueguen, Marielle.
In October and November 2003 a bloom of the toxic dinoflagellate Alexandrium catenella was observed in the North-east zone of Thau lagoon (French Mediterranean coast). Sea water samples were collected every hours to evaluate time-related variations of phytoplankton concentrations and to compare the relative ratio of A. catenella versus other phytoplankton species during the outbreak. From these observations, trials using recirculated sea water systems were performed to: i) evaluate the physiological effects on oyster of increasing proportions of A. catenella within a mixed microalgal diet where the non-toxic diatom Thalassiosira weissflogii was present at concentration: 1500 cells ml(-1), ii) compare the effect of two temperatures (12 degrees C and 18...
Tipo: Text
Palavras-chave: Mediterranean Sea; Alexandrium catenella; Toxin content; Model; Crassostrea gigas; Paralytic shellfish poisoning.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2624.pdf

Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum
Provedor de dados: 5
Autores: Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher.
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment...
Tipo: Text
Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf

Spirolide uptake and detoxification by Crassostrea gigas exposed to the toxic dinoflagellate Alexandrium ostenfeldii
Provedor de dados: 5
Autores: Medhioub, Walid; Lassus, Patrick; Truquet, Philippe; Bardouil, Michele; Amzil, Zouher; Sechet, Veronique; Sibat, Manoella; Soudant, Philippe.
Oysters (Crassostrea gigas) were exposed 4 days to cultures of the toxic dinoflagellate Alexandrium ostenfeldii (strain CCMP1773) that produces spirolides belonging to fast acting toxins (FAT) and let depurate for 7 days. During depuration, oysters were either fed the non-toxic algae Isochrysis galbana Tahitian clone (T. Iso) or starved. The objectives of this experiment were to evaluate (i) spirolide uptake and depuration by oysters (ii) spirolide effects on oysters and (iii) oyster recovery according to food supply during depuration. A. ostenfeldii cells were filtered and ingested by oysters while faeces contained numerous intact cells of the toxic diet. This suggested that ingested cells were not totally digested by oysters. Contents of spirolides (SPX)...
Tipo: Text
Palavras-chave: Spirolides; Alexandrium ostenfeldii; Contamination; Depuration; Crassostrea gigas; Inflammatory responses.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20953/18740.pdf

Hydrologie et distribution de dinophysis en juin 1988, dans la frange côtière nord-Gascogne
Provedor de dados: 5
Autores: Lassus, Patrick; Truquet, Philippe; Le Dean, Loic; Bardouil, Michele.
During the oceanographic cruise of June 1988, phytoplankton and correlated hydrological parameters were studied in the coastal area between Camaret and Ile d'Oléron. 62 stations were sampled in the 0-30 m layer within the 50 m depth limited coastal area. Observations focused on two toxic dinoflagellate species: Dinophysis spp and Gyrodinium aureolum. Dinophysis spp is more abundant in the Loire/Vilaine area, which is.characterized by high surface Nitrate levels, high 30 m depth Phosphate levels and Chlorophyll a amounts higher than elsewhere during the survey. This particuliar pattern can be related to the Nutrient outputs of Loire and Vilaine Rivers. Max. Dinophysis concentrations were observed in Vilaine bay (very high Phosphate levels) and in Douarnenez...
Tipo: Text
Palavras-chave: Phytoplancton; Dinophysis; Gyrodinium aureolum; Hydrologie; Sels nutritifs; Campagne côtière; Nord Gascogne; Phytoplankton; Dinophysis; Gyrodinium aureolum; Hydrology; Nutrients; Coastal cruise; Northern Biscay.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00432/54323/58896.pdf

Etudes préliminaires sur la sensibilité d'organismes planctoniques marins vis à vis de deux insecticides et d'un herbicide
Provedor de dados: 5
Autores: Marcaillou Le Baut, C; Bardouil, Michele; Bocquene, Gilles; Le Dean, Loic; Truquet, Philippe.
This work is the preliminary synthesis of some toxicological tests conducted on three pesticides. This recapitulation would not have been possible without the contribution of our trainees, Misses V. Perles and H. Le Boulicot and MM. M. Falchier and G. Gaty who contributed largely to this study, supervised by the laboratory staff. The obtained results cannot be considered as definitive. The urge to answer the needs did not leave us enough time to fine tune and/or complete the results; for example we still have not determined the lethal dose for 50% of the individuals. [...]
Tipo: Text
Ano: 1983
URL: https://archimer.ifremer.fr/doc/1983/rapport-4575.pdf