Consulta: creatorFacets:"Barreto,Marliton R."
Registros recuperados: 9
Data/hora: 13/06/2024 03:51:03

Associação de bioensaios e caracterização molecular para seleção de novos isolados de Bacillus thuringiensis efetivos contra Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 82
Autores: Fatoretto,Júlio C.; Sena,Janete A.D.; Barreto,Marliton R.; Lemos,Manoel V.F.; Boiça Junior,Arlindo L.
A lagarta de Spodoptera frugiperda (J. E. Smith) é uma das principais pragas do milho e para seu controle o Bacillus thuringiensis se destaca por sua atividade entomopatogênica. Este trabalho objetivou a caracterização molecular de isolados de B. thuringiensis quanto à presença do gene cry1 e a avaliação da sua eficiência no controle de lagartas de S. frugiperda. Nas análises da PCR, foi utilizado o Gral-cry1 para confirmação da presença do gene cry1 nos 115 isolados. Uma suspensão de 3 x 10(8) esporos/ml banhou a dieta utilizada para alimentação de 30 lagartas por isolado, com três repetições. A identificação do tipo de genes cry1 dos diferentes isolados foi realizada para cinco sub-classes de genes e análises de regressão linear foram realizadas para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lagarta-do-cartucho; Cry1; Bactéria; Prospecção; Gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000500015

Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD
Provedor de dados: 82
Autores: Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H.
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect pathology; Fall armyworm larvae; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010

Bacillus thuringiensis survey in Brazil: geographical distribution and insecticidal activity against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 82
Autores: Valicente,Fernando H.; Barreto,Marliton R.
A total of 3408 strains of Bacillus thuringiensis were collected from 1448 soil samples in 10 Brazilian states, four different geographical regions, covering 96 counties. These strains were evaluated against Spodoptera frugiperda (J.E. Smith) larvae. Only 62% killed between 81% and 100% and 1758 caused no mortality. Highest proportion of efficient strains (larval mortality above 75%) was found from the total isolated per region in the South Region (16.6%), followed by Western Central (3.1%), Southeast (1.1%) and Northeast Region (0.4%).
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Insect; Bacteria; Native isolate; Biological control; Pathology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000400014

Identificação através de PCR dos genes CryI de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 44
Autores: Valicente,Fernando H.; Barreto,Marliton R.; Vasconcelos,Maria J. V. de; Figueiredo,José E.F. de; Paiva,Edilson.
Fall armyworm, Spodoptera frugiperda (J. E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to Identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Bioassays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryI general primers and their proteins were analised by SDS-PAGE eletrophoresis. All strains present positive results for cryI genes. To further characterize these strains, specific cryI primers were employed. PCR technique showed that some strains harbour the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; CryI genes; Biocontrol; Fall armyworm.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000100018

Comportamento de corte e cópula do predador Supputius cincticeps (Stal) (Heteroptera: Pentatomidae) em laboratório
Provedor de dados: 44
Autores: Mendonça,Fernando A. C.; Barreto,Marliton R.; Assis Jr.,Sebastião; Marsaro Jr.,Alberto L.
The mating behavior of the predator Supputius cincticeps (Stal) was investigated in the laboratory. Courtship and copulation descriptions were based on direct observations of 40 virgin couples. Results showed that males begun courtship by moving the antennae and walking toward females, immediately after they noticed their presence. Then, males mounted females and tried to copulate. In many cases, males were rejected by females. When copulation was successful, males placed the edeagus into females, turned 180º and assumed a position opposite to the female.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Asopinae; Mating; Biological control.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100030

Leveduras associadas a Spermologus rufus Boheman (Coleoptera: Curculionidae)
Provedor de dados: 44
Autores: Barreto,Marliton R.; Barreto,Eriana S.; Anjos,Norivaldo.
Larvae and adults of Spermologus rufus Boheman can cause severe damage to seeds of Brazilian pine, Araucaria angustifolia. In this study, yeasts were isolated from seeds of A. angustifolia and from the digestive tract of S. rufus to verify their similarity and determine the existence of feeding sites in order to elaborate an artificial diet for this insect. Pichia spp. were found in seeds and Pichia spp. and P. stipitis Pignal in the digestive tract of S. rufus. The low variability among observed microorganisms suggests a food preference, which can facilitate the elaboration of artificial diets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Coleoptera; Curculionidae; Yeast; Pichia; Artificial diets; Brazilian pine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000200017

Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae
Provedor de dados: 44
Autores: Barreto,Marliton R.; Loguercio,Leandro L.; Valicente,Fernando H.; Paiva,Edilson.
Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Biological control; Fall armyworm; Vegetative insecticidal proteins; Vip genes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000400010

Comportamento reprodutivo de Spermologus rufus Boheman (Coleoptera: Curculionidae)
Provedor de dados: 44
Autores: Barreto,Marliton R.; Anjos,Norivaldo; Della Lucia,Terezinha M. C.
Spermologus rufus Boheman (Coleoptera: Curculionidae) is an insect which attacks cacao nuts, oleaginous pits and Araucaria angustifolia (Bert.) Kuntze seeds. In this study the reproductive behavior of S. rufus was investigated to provide useful knowledge to support control measures of this insect. Research was carried out under laboratory conditions. Twenty-two couples of newly emerged adults were individualized in Wheaton flasks containing A. angustifolia seeds for oviposition and feeding. Mating was observed every day until the insects died. Females oviposited in the seeds; 80.4% of these seeds had only one egg. Eggs were mostly found in the median and more enlarged region of the seed and at the insertion point. Pre-oviposition period was, on average,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Courtship oviposition.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000200022

Levantamento dos inimigos naturais da lagarta do cartucho do milho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), na região de Cascavel, PR
Provedor de dados: 44
Autores: Valicente,Fernando H.; Barreto,Marliton R.
A survey of the natural enemies of S. frugiperda larvae was done during the years of 1992/93 and 94/95, in Cascavel region, Parana State. The most frequent parasitoids were Campoletis sp. (Hymenoptera: Ichneumonidae), which reached 47.0% of parasitism and Archytas marmoratus (Diptera: Tachinidae), which reached 15.4%. Of all Baculovirus found, 10.8% were NPV (nuclear polihedrosis virus) and only 1% was GV (granulosis virus).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Parasitoids; Baculovirus; Fungi.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000200018