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Innovative molecular approach to the identification of Colossoma macropomum and its hybrids
Provedor de dados: 42
Autores: Gomes,Fátima; Schneider,Horacio; Barros,Claudene; Sampaio,Dioniso; Hashimoto,Diogo; Porto-Foresti,Fábio; Sampaio,Iracilda.
Tambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as "Tambacu" and "Tambatinga". Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (α-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pisciculture; Multiplex PCR; Mitochondrial DNA; Genetic bottleneck.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200020