Consulta: creatorFacets:"Berthe, Franck"
Registros recuperados: 61
Data/hora: 06/06/2024 00:46:12

Use of molecular tools for mollusc disease diagnosis
Provedor de dados: 5
Autores: Berthe, Franck; Burreson, Eugene; Hine, Mike.
Recently, an increasing number of research teams are engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues.
Tipo: Text
Palavras-chave: Molecular tools; Mollusc disease; Diagnosis; Pathology.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-3291.pdf

Screening of Vibrio isolates to develop an experimental infection model in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Gay, Melanie; Berthe, Franck; Leroux, Frederique.
In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C, gigas mortality rates in experimental challenges. Although the study...
Tipo: Text
Palavras-chave: Crassostrea gias; Antagonism; Agonism; Virulence; Vibrio splendidus.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3174.pdf

Rapport sur la pathologie des pénéides en Nouvelle-Calédonie. Eat et perspectives du programme d'assistance à la filière crevette de Nouvelle-Calédonie par la recherche en pathologie
Provedor de dados: 5
Autores: Berthe, Franck.
La pénéiculture calédonienne connait, depuis 1993, de sévères difficultés de développement et son expansion, spectaculaire au cours des six dernières années, est aujourd'hui considérablement ralentie. Le «syndrome 93», terme consacré par l' usage, affecte les élevages dans leur phase dite de grossissement, et se traduit par des taux de survie inférieurs aux seuils de rentabilité des fermes. Les pertes infligées à la filière mettent celle-ci en péril.C'est pourquoi un programme d'assistance par la recherche en pathologie des pénéides a été élaboré afin de répondre à ces difficultés. Le but de cette démarche est d'établir un plan de lutte basé sur la détection précoce de situations critiques, caractérisées par la présence d'un agent pathogène (diagnostic) et...
Tipo: Text
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00451/56240/57800.pdf

Caractérisation de bactéries pathogènes, Vibrio splendidus, isolées de bivalves marins
Provedor de dados: 5
Autores: Gay, Melanie; Waechter, Magali; Lambert, Christophe; Escoubas, Jean-michel; Cochennec, Nathalie; Nicolas, Jean-louis; Berthe, Franck; Le Roux, Frédérique.
Nowadays, cupped oyster, Crassostrea gigas, production is the main shellfish production in France. Spats cultivated on most farms are usually collected in the wild, but hatchery spats are becoming more numerous, and represent around 10% of today's cultivated spats. During the summer period, when the temperatures are higher, high mortality rates have been regularly observed in cupped in oyster C. gigas (60 to 100%) spats since 1991, both in hatcheries-nurseries and in their natural habitat. Over the last five years, these episodes have become a big concern for all the professionals.
Tipo: Text
Palavras-chave: Crassostrea gigas; Pathogène; Vibrio splendidus; Bactériologie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3277.pdf

First record of Marteilia sp in mussels Mytilus galloprovincialis in Croatia
Provedor de dados: 5
Autores: Zrncic, Snjevana; Le Roux, Frédérique; Oraic, Drazen; Sostaric, Branko; Berthe, Franck.
Marteiliosis is a disease of molluscs caused by Marteilia refringens in Europe and M. sydneyi in Australia. During routine examination of cultured mussels Mytilus galloprovinciallis in the northern Adriatic, the occurrence of Marteilia sp. was recorded with a prevalence of 5 %. This parasite was not detected in flat oysters reared in the same area. The affiliation of the detected parasite in M. galloprovinciallis was confirmed by in situ hybridization using a M. refringens probe, specific at the genus level. DNA of these infected mussels originating from the same area will be used to clarify the taxonomic position of this species within the genus Marteilia using a molecular approach.
Tipo: Text
Palavras-chave: Taxonomy; Detection; Mytilus galloprovincialis; Parasite; Marteilia maurini.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-817.pdf

Mikrocytos roughleyi taxonomic affiliation leads to the genus Bonamia (Haplosporidia)
Provedor de dados: 5
Autores: Cochennec, Nathalie; Reece, K.; Berthe, Franck; Hine, Mike.
Microcell-type parasites of oysters are associated with a complex of diseases in different oyster species around the world. The etiological agents are protists of very small size that are very difficult to characterize taxonomically. Associated lesions may vary according to the host species, and their occurrence may be related to variations in tissue structure. Lesion morphology cannot be used to distinguish the different agents involved. Ultrastructural observations on Mikrocytos roughleyi revealed similarities with Bonamia spp., particularly in regard to the presence of electron-dense haplosporosomes and mitochondria, whose absence from M mackini also indicate that M roughleyi and M mackini are not congeneric. A partial small subunit (ssu) rRNA gene...
Tipo: Text
Palavras-chave: Small subunit rDNA; Taxonomy; Microcell; Bonamosis; Saccostrea glomerata; Mikrocytos roughleyi.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-2816.pdf

Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events
Provedor de dados: 5
Autores: Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck.
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast...
Tipo: Text
Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf

The French Polynesian experience
Provedor de dados: 5
Autores: Berthe, Franck; Prou, Jean.
French Polyncsia is currently the world's largest producer of cultured black pearls with exports worth US$150 millions annually. The activity has become of prime socio-economic importance the archipelagos with strong support and control of the government. In the mid-1980s, mass mortality of the black-lipped pearl oyster, Pinctada margaritifera, has occurred in several atolls and challenged the development of pearl farming activity. Consequent Studies and surveys have described severa! pathologica! conditions. None of those is considered to pose significant threat to the industry. However, they are monitored by a surveillance program in order to prevent and control emerging diseases. The governmemt of French Polynesia has developed efforts to maintain high...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00353/46396/46032.pdf

Development and validation of DNA-based diagnostic techniques with particular reference to bivalve mollusc pathogens
Provedor de dados: 5
Autores: Berthe, Franck.
The International Aquatic Animal Health Code of the OIE (the World Organization for Animal Health) includes serious pathogens that have been causing important losses in the mollusc aquaculture industry throughout the world. This list also meets the ones established by the European Union regulation.
Tipo: Text
Palavras-chave: Pathogens; Techniques; Diagnostics; Pathology; Molluscs.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3213.pdf

Vibriosis in bivalves : review of recent molecular, biochemical, and physiological studies
Provedor de dados: 5
Autores: Paillard, Christine; Nicolas, Jean-louis; Le Chevalier, Patrick; Lambert, Christophe; Le Boulay, Christine; Berthe, Franck; Haras, Dominique.
The emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For examples, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crass()strea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities.
Tipo: Text
Palavras-chave: Pathogenic vibrios; Bivales; Vibriosis; Bacteriology.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3287.pdf

Arbitrarily primed PCR to type Vibrio spp. Pathogenic for shrimp
Provedor de dados: 5
Autores: Goarant, Cyrille; Merien, Fabrice; Berthe, Franck; Mermoud, Isabelle; Perolat, Philippe.
A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR...
Tipo: Text
Palavras-chave: Vibrio spp.; Shrimp; Molecular typing.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-2734.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Journées du Département RA - Compte-rendu de la revue du thème "santé des cheptels"
Provedor de dados: 5
Autores: Grizel, Henri; Berthe, Franck.
Les 21 et 22 juin 2000, le Département des Ressources Aquacoles a consacré ses journées annuelles à une revue du thème "santé des cheptels" et du dispositif de recherche en pathologie sur les filières poissons, crustacés et mollusques, domaine très stratégique pour l'aquaculture marine française. Les objectifs des ces journées, rappelés par Yves Harache, Directeur du Département, étaient les suivante : • Dresser le bilan des actions de recherche menées par l'Ifremer en pathologie des espèces aquacoles,  • Repréciser la stratégie (objectifs/moyens) du Département des Ressources Aquacoles sur le thème "santé des cheptels",  • Programmer les actions de recherche à venir, • Stimuler les interactions entre groupes de recherche et laboratoires impliqués. Le...
Tipo: Text
Palavras-chave: Pathologie; Ecotoxicologie; Mortalités; Ecopathologie; Poissons; Crustacés; Mollusques; Nacres.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00164/27547/25711.pdf

Molluscan pathogens of concern to ASEAN
Provedor de dados: 5
Autores: Bondad-reantaso, Melba; Berthe, Franck.
Pacific oysters were introduced into west coast of the USA from Matsushima Bay in Japan, infected with low levels of Haplosporidiumsp. identical to H.nelsoni(MSX) which causes high mortalities of Eastern oyster (C. virginica). A highly specific and sensitive DNA probe for H. nelsonican also detect Haplosporidium sp. in C.gigas of western US and Japan (Burreson and Stokes 2000).In 2002, H. nelsoni was detected in Japan using the same probe (Kamaishi and Yoshinaga 2002).Lesson: it appears that H. nelsoni does not cause serious disease in Pacific oysters; H. nelsoniis now speculated to have been introduced into the Pacific US by apparently healthy but infected C. gigas. Infected C. gigaswere introduced onto the east coast of the US where the parasite shifted...
Tipo: Text
Palavras-chave: Pathogens; Diseases; Impact; Introductions; Transfers; Molluscan Pathogens.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3294.pdf

Bonamia exitiosa Hine et al., 2001 is proposed instead of B. exitiosus as the valid name of Bonamia sp infecting flat oysters Ostrea chilensis in New Zealand
Provedor de dados: 5
Autores: Berthe, Franck; Hine, Mike.
A parasite of the dredge oyster Ostrea chilensis Philippi, previously known as Bonamia sp., was recently described and named Bonamia exitiosus, based on ultrastructural and molecular evidence. The rules governing the names of organisms require correspondence of gender, and the name must be changed from B. exitiosus to B. exitiosa.
Tipo: Text
Palavras-chave: Zoological nomenclature; Bonamia exitiosa.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-2843.pdf

Epidémiologie moléculaire des espèces Marteilia en Europe
Provedor de dados: 5
Autores: Le Roux, Frédérique; Audemard, Corinne; Cochennec, Nathalie; Thebault, Anne; Berthe, Franck.
Responsible for massive epizootics, the Marteilia type parasites are very dangerous infectious agents for oysters. As a matter of fact, in France the spreading out of the parasitosis caused by Marteilia refringens disturbed dangerously the flat oyster, Ostrea edulis, production; destroying a large percentage of the farms on the Atlantic coast. The fight against marteiliose implies a good management of the contamination risk, that is to say the identification of the parasite(s), the characterization of the host(s), vector(s) or reservoir(s) and the understanding of the parasite's transmission mode.
Tipo: Text
Palavras-chave: Bivalves; Biologie Moléculaire; Marteilia refringens; Epidémiologie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3271.pdf

Prevalence and infection intensity of the ovarian parasite Marteilioides chungmuensis during an annual reproductive cycle of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Ngo, Thao; Berthe, Franck; Choi, Kwang-sik.
The occurrence of Marteilioides chungmuensis, a protozoan paramyxean parasite in the reproductive system of the Pacific oyster Crassostrea gigas, was observed at Gosung Bay, Korea. Seasonal variation in gonad development was investigated in a suspended cultured oyster population. Gametogenesis began in February and first-spawning was observed between mid and late June when surface water temperature reached 22 to 25degreesC. Spawning activity extended from mid June to late September, with 2 marked spawning peaks in June and August. Histological examination indicated that gonad development paralleled seasonal fluctuations in water temperature. Spawning in late June was partly associated with a sudden drop in salinity due to large freshwater inputs to the Bay...
Tipo: Text
Palavras-chave: Korea; Ovary parasite; Marteilioides chungmuensis; Gonad development; Crassostrea gigas.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-2848.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

Cg-TIMP, un inhibiteur de metalloproteinase isolé chez Crassostrea gigas : étude de son rôle dans la réparation des lésions et dans les mécanismes de défense
Provedor de dados: 5
Autores: Escoubas, Jean-michel; Montagnani, Caroline; Le Roux, Frédérique; De Lorgeril, Julien; Berthe, Franck; Bachere, Evelyne.
We isolated complementary DNA (Cg-TIMP) from Crassostrea gigas that codes for a protein highly homologous to vertebrate tissue inhibitors of metalloproteinases (TIMPs). In vertebrates, TIMPs are multifunctional proteins that play a key role in extracellular matrix metabolism. They are involved in physiological processes (e.g. embryonic development, damage repair, angiogenesis) and pathological processes (e.g. inflammatory reactions, cancer).
Tipo: Text
Palavras-chave: Proteine; Cg TIMP; ADN; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3268.pdf

Les parasites du groupe "Mikrocell" : Qui est qui?
Provedor de dados: 5
Autores: Cochennec, Nathalie; Le Roux, Frédérique; Berthe, Franck; Thebault, Anne.
Bonamiose, a disease caused by the protozoa Bonamia ostreae, has destroyed the flat oyster Ostrea edulis production, in France but also in Europe and in the United States since 1979. Another species of Bonamia has been monitored in New-Zealand, in Australia and lately in Chile. In Canada and Australia, two other parasites have been described in cupped oyster: Mikrocytos mackini and Mikrocytos roughleyi. All these small size protozoa are grouped under the name "Mikrocell". For most of them, light and electronic microscope descriptions have been realised. Based on those criteria, different taxonomic positions have been proposed. However, these analysis are still questioned today.
Tipo: Text
Palavras-chave: Bivalves; Haplosporidia; Parasites; Pathologie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3270.pdf

Delta de l'Ebre is a natural bay model for Marteilia spp. (Paramyxea) dynamics and life-cycle studies
Provedor de dados: 5
Autores: Carrasco, N; Arzul, Isabelle; Berthe, Franck; Fernandez Tejedor, M; Durfort, M; Furones, M.
Marteilia spp. are paramyxean parasites that affect several bivalve species of economic interest, such as Ostrea edulis and Mytilus galloprovincialis. Certain aspects of Marteilia spp., such as their life cycle and host affinity and infection dynamics, still remain unknown. The 'Delta de l'Ebre' constitutes a natural model for the study of the life cycle of the parasite Marteilia, since uninfected mussels and flat oysters immersed in the bays can become infected. This, along with the geographical and ecological characteristics of the bays, make it a very interesting location to study the Marteilia life cycle. Preliminary results concerning marteiliosis, mainly in mussels, such as prevalence dynamics, infectious periods, host affinity and host intermediate...
Tipo: Text
Palavras-chave: Ebre delta bays; Host specificity; Intermediate host; Mytilus galloprovincialis; Paramyxea; Marteilia.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3880.pdf

Report about mollusc diseaes
Provedor de dados: 5
Autores: Berthe, Franck.
Of the 75 laboratories contacted through the survey, 54 answered, 14 of which are partially or totally devoted to mollusc diseases. In fact, only 4 laboratories are devoted full-time to this work. Of the 14 laboratories that declared to work on the diagnosis of mollusc diseases, 12 stated that they worked with mussels, 12 with oysters, 9 with clams and 8 with other mollusc species. This is coherent with available production information. According to production data in 2001 (FAO source), the main mollusc species in the region are the blue mussel, Mytilus edulis (297,485 tonnes), the Mediterranean mussel, Mytilus galloprovincialis (131,014 tonnes), the Pacific cupped oyster, Crassostrea gigas (127,323 tonnes, an important part of which is produced on the...
Tipo: Text
Palavras-chave: Bonamia existiosus; Bonamis ostreae; Bonamiosis; Marteilia sydneyi; Marteilia maurini; Marteilia refringens; Marteiliosis; Perkinsus marinus; Perkinsus olseni; Perkinsus atlanticus; Perkinsosis; Vibriosis; Herpes like virus; Oysters; Pathology; Mollusc diseases.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3300.pdf

Pipetman et bottes en caoutchouc à la recherche d'hôte(s) de Marteilia refringens
Provedor de dados: 5
Autores: Audemard, Corinne; Le Roux, Frédérique; Sauriau, Pierre-guy; Sautour, Benoît; Combes, C.; Coustau, C.; Blachier, Philippe; Berthe, Franck.
Over the last thirty years, the French production of flat oyster Ostrea edulis dropped from15,000 tons/year to 1,500 tons. Marteilia refringens, a parasite of the flat oyster's digestive gland, is partly responsible for this drop. The fight against this pathogen agent requires a better knowledge of its parasitic cycle. The main hypothesis is the existence of a heteroxenous cycle, that is to say a cycle involving more than one host. However, for thirty years this or these hosts have not been found, and the cycle of Marteilia refringens remains unknown.
Tipo: Text
Palavras-chave: Claires ostréicoles; Parasite; Détection; Biologie moléculaire; Marteilia refringens.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3272.pdf

Development of a TaqMan PCR assay for the detection of Bonamia species
Provedor de dados: 5
Autores: Corbeil, Serge; Arzul, Isabelle; Diggles, Ben; Heasman, Mike; Chollet, Bruno; Berthe, Franck; Crane, Mark St. J.
The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New...
Tipo: Text
Palavras-chave: Oyster; Real time TaqMan PCR assay; Bonamia spp.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1980.pdf

Some regulatory issues related to perkinsosis
Provedor de dados: 5
Autores: Berthe, Franck.
Perkinsosis is one of the OIE listed diseases of molluscs caused by Perkinsus marinus and P. olseni. Consequently, molluscs originating from endemic areas should not be transferred into areas free of the disease. Impediments to the implementation of international zoosanitary regulation will be reviewed.
Tipo: Text
Palavras-chave: Pathology; Molluscs; Perkinsus olseni; Perkinsus marinus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3284.pdf

Ostrea conchaphila : a natural host of Bonamia ostreae ?
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Garcia, Celine; Robert, Maeva; Joly, Jean-pierre; Miossec, Laurence; Berthe, Franck.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00448/55998/57502.pdf

Que savons-nous des Marteillia?
Provedor de dados: 5
Autores: Berthe, Franck; Haffner, Philippe; Zerabib, Maamar; Rodriguez, David.
Marteilia refringens, a parasitic protist, is partially responsible for the drastic reduction in French production of the European flat oyster (Ostrea edulis) in the 1970s and still constitutes a major obstacle for revitalizing coastal farming of this species.
Tipo: Text
Palavras-chave: Bivalves; Ostrea edulis; Protiste; Parasite; Marteilia refringens; Pathologie.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3247.pdf

Bilan 1999 du Réseau REPAMO - Réseau National de Surveillance zoosanitaire des Mollusques marins
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Grizel, Henri; Berthe, Franck; Cochennec, Nathalie; Renault, Tristan; Le Roux, Frederique.
Le réseau REPAMO (Réseau de Pathologie des Mollusques) est un réseau de santé animale, qui s'occupe des coquillages marins du littoral français métropolitain. Les mollusques marins des côtes françaises représentent une ressource par la pêche, mais sont surtout une part importante de l'aquaculture. Les pertes liées aux maladies dans ce domaine d'activité sont aujourd'hui considérées comme l'un des principaux facteurs limitants de son développement au niveau mondial. Deux exemples récents suffisent à illustrer ce danger pour l'ostréiculture française : • La culture de l''huître plate Ostrea edulis, déjà atteinte par un parasite, Marteilia refringens, est passée en quelques années de 20 000 tonnes à 1800 tonnes, suite à une épizootie. En effet à la fin des...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00098/20903/18522.pdf

Taxinomie et épidémiologie moléculaires du parasite Marteilia refringens Grizel et al. 1974 : intérêt pour la gestion du risque sanitaire en conchyliculture
Provedor de dados: 5
Autores: Berthe, Franck.
Infectious diseases are currently recognised as a major limiting factor to development and sustainability of aquaculture of molluscs. The study is focused on the European flat oyster, Ostrea edulis and its paramyxean parasite Marteilia refringens. Results are contributing to the prevention of risk associated to infected stocks imports from endemic zones, by developing molecular diagnostic tools that are specific, sensible and reliable. Phylogeny and taxonomy of Marteilia refringens, previously impeded by microscopy limits, were clarified by sequencing the rDNA SSU, small sub-unit in the rRNA gene cluster. These new insights have practical implications discussed in a pragmatic polyphasic approach involving pathology and epidemiology. This approach led us to...
Tipo: Text
Palavras-chave: Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Pathologie; Phylogénie moléculaire; Taxinomie; Epidémiologie; Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Molluscs diseases; Molecular phylogeny; Taxonomy; Epidemiology.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00109/21980/19594.pdf

Diseases in mollusc hatcheries and their paraedox in health management
Provedor de dados: 5
Autores: Berthe, Franck.
Global molluscan aquaculture production is continuously increasing, dominated by five species, among which the Pacific cupped oyster, Crassostrea gigas, predominates. Hatchery production is accompanying this increase to assist consistent availability of juveniles for restocking, fishery enhancement, genetic improvements as well as for species diversification. Hatchery development contributes significantly to the demand for international transfers of live molluscs; a consequence of which pathogen transfer via transfer of live molluscs is currently recognised as a major cause of epizootic disease outbreaks. Diseases are a primary constraint to mollusc aquaculture growth and sustainability, severely impacting socioeconomic development in many countries....
Tipo: Text
Palavras-chave: Mollusca; Crassostrea gigas; Risks; Mollusc culture; Husbandry diseases; Hatcheries; Disease control.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3289.pdf

Perkinsosis in Europe : current issues and research needs
Provedor de dados: 5
Autores: Berthe, Franck; Choi, K. S.; Figueras, Antonio; Soudant, Philippe; Villalba, A.
The aim of this workshop was to review current issues posed by perkinsosis in Europe with particular emphasis on taxonomv. epidemiology and impact an mollusc stocks, and identify where new developments in research are likely to improve our knowledge and understandings.
Tipo: Text
Palavras-chave: Parasitic diseases; Pathogens; Geographical distribution; Fish diseases; Perkinsus marinus; Pathology.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-3290.pdf

Pacem in terris pathogenibus bonae voluntatis: molluscs-pathogens relationships prospects
Provedor de dados: 5
Autores: Berthe, Franck.
In 1999, the world aquaculture production of molluscs was estimated at 9.4 million tonnes which represents approximately a 23% contribution to the global aquaculture production. Aquaculture of molluscs has been growing at an average annual rate of 11% over the past decades, although some activities - such as aquaculture of pectens or abalones - were growing even more rapidly. In many countries, there is a significant mollusc aquaculture, traditionally based on wild stocks. However, these natural populations frequently do not fulfil market demand, because of low value of the product, over-fishing of the resource and/or mass mortality outbreaks caused by diseases. A frequent answer to this situation has been the introduction of new stocks or new species in...
Tipo: Text
Palavras-chave: Mortality; Mollucs; Pathogen.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2780.pdf

Programme Pathologie - Rapport scientifique Année 1994 - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 94 RPC R 62
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit.
Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie....
Tipo: Text
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf

Marteiliosis in molluscs: A review
Provedor de dados: 5
Autores: Berthe, Franck; Leroux, Frederique; Adlard, Robert; Figueras, Antonio.
Among mollusc pathogens, paramyxeans are an important group of protistan parasites belonging to the genera Marteilia and Marteilioides. Martelia refringens and M. sydneyi are of particular concern given their potential impact on mollusc aquaculture world-wide. Aber disease and QX disease are currently listed by the OIE, the World Organisation for Animal Health. After more than thirty years of existence in the scientific literature, these organisms still pose questions and research challenges to investigators. This paper reviews current knowledge of the group and key references. The review was focused on taxonomy, epidemiology, pathology and potential control methods for these organisms in order to enhance understanding of paramyxean infection issues in...
Tipo: Text
Palavras-chave: Paramyxean parasites; Diagnosis; Pathology; Epidemiology; Taxonomy.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-406.pdf

Molecular detection of the oyster parasite Mikrocytos mackini, and a preliminary phylogenetic analysis
Provedor de dados: 5
Autores: Carnegie, Ryan; Meyer, Gary; Blackbourn, Janice; Cochennec, Nathalie; Berthe, Franck; Bower, Susan.
The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M mackini, the PCR assay was validated against standard histopathological diagnosis, and a preliminary phylogenetic analysis of the M mackini small-subunit ribosomal RNA gene (SSU rDNA) was undertaken. A PCR designed specifically not to amplify host DNA generated a 544 bp SSU rDNA fragment from M mackini-infected oysters and enriched M. mackini cell isolates, but not from uninfected control oysters....
Tipo: Text
Palavras-chave: Fluorescent in situ hybridization; PCR validation; Mikrocytos mackini; Denman Island disease.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-2814.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Bonamia exitiosus n. sp (Haplosporidia) infecting flat oysters Ostrea chilensis in New Zealand
Provedor de dados: 5
Autores: Hine, P; Cochennec, Nathalie; Berthe, Franck.
Bonamia sp. is a pathogenic parasite that occurs in the haemocytes of dredge oysters Ostrea chilensis Philippi in New Zealand, Ultrastructurally it resembles other haplosporidians in the possession of haplosporosomes, haplosporogenesis, persistence of mitotic microtubules during interphase and of the nuclear envelope during mitosis, and occurrence of a diplokaryotic or multi-nucleate plasmodial stage. Another stage containing a large vacuole derived from enlargement of 1 or more mitochondria has not previously been described from other haplosporidians. It most closely resembles B, ostreae Pichot et al., 1979, which parasitises and is pathogenic in haemocytes of European flat oysters, O. edulis. However, B. ostreae is smaller and denser, and has fewer...
Tipo: Text
Palavras-chave: New Zealand; 18S rDNA; Ultrastructure; Ostrea chilensis; Bonamia exitiosus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-818.pdf

Molecular characterisation of an Australian isolate of Bonamia exitiosa
Provedor de dados: 5
Autores: Corbeil, Serge; Arzul, Isabelle; Robert, Maeva; Berthe, Franck; Cochennec, Nathalie; Crane M, Mark.
An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.
Tipo: Text
Palavras-chave: Small subunit rRNA operon; Flat oyster; Ostrea angasi; Bonamia species.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1981.pdf

Ultrastructure of Mikrocytos mackini, the cause of Denman Island disease in oysters Crassostrea spp. and Ostrea spp. in British Columbia, Canada
Provedor de dados: 5
Autores: Hine, P; Bower, S; Meyer, G; Cochennec, Nathalie; Berthe, Franck.
An ultrastructural study was carried out on Mikrocytos mackini, the cause of Denman Island disease in Pacific oysters Crassostrea gigas in western Canada. Three forms were identified, quiescent cells (QC), vesicular cells (VC) and endosomal cells (EC), QC occurred in the vesicular connective tissue (VCT), haemocytes (hyalinocytes), adductor and heart myocytes, and extracellularly, They had a central round to ovoid nucleus, &lt;7 cisternae of inactive nuclear membrane-bound Golgi, few vesicles and lysosome-like bodies. VC were rarely extracellular and usually occurred in adductor and heart myocytes, in close association with host cell mitochondria. The contents of the host cell mitochondria appeared to pass through a tubular extension into the cytoplasm of...
Tipo: Text
Palavras-chave: Denman Island disease; Amitochondriate protozoan; Ultrastructure; Crassostrea gigas; Mikrocytos mackini.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-815.pdf

Bilan du réseau pathologie des mollusques - REPAMO 1996
Provedor de dados: 5
Autores: Berthe, Franck.
La réglementation zoosanitaire vise à protéger les cheptels en limitant les risques de développement et de propagation des maladies infectieuses. La traduction en droit français des Directives Européennes 91167/CE et 95/70/CE confère au Réseau de Pathologie des Mollusques de l'IFREMER (REPAMO) la charge de contrôler la santé des invertébrés marins d' intérêt commercial sur le territoire national. Les objectifs de ce réseau sont les suivants :  - la réalisation des analyses permettant de classer les zones pour les maladies obligatoirement déclarables, - la surveillance de base pour l'ensemble du cheptel français, - l'étude des causes de mortalité anormale, - le contrôle des produits échangés entre les pays de l'Union Européenne et la France et les pays...
Tipo: Text
Palavras-chave: Mollusques bivalves; Pathologie; Réseau surveillance; Zoosanitaie.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00199/31046/29463.pdf

Could Mikrocytos roughleyi be a mis-classified haplosporidian?
Provedor de dados: 5
Autores: Cochennec, Nathalie; Le Roux, Frédérique; Berthe, Franck; Hine, Mike.
Bonamiosis refers to diseases of oysters caused by Bonamia ostreae in the Northern Hemisphere and Bonamia sp. in the Southern Hemisphere. Similarly, Mikrocytosis usually refers to diseases of oysters caused by Mikrocytos mackini on the west coast of Canada and Mikrocytos roughleyi on the east coast of Australia.
Tipo: Text
Palavras-chave: Oysters; Diseases; Pathology; Mikrocytos roughleyi.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3286.pdf

Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens
Provedor de dados: 5
Autores: Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck.
Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of...
Tipo: Text
Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf

First record of a Marteilia parasite (Paramyxea) in zooplankton populations from a natural estuarine environment
Provedor de dados: 5
Autores: Carrasco, Noelia; Lopez, Immaculada; Alcaraz, M; Furones, M. Dolores; Berthe, Franck; Arzul, Isabelle.
The life cycle of the species forming the genus Marteilia (Phylum Paramyxea), which are marine protozoan parasites of molluscs, is still poorly known, although there is evidence of the need for intermediate hosts. To date, only the calanoid copepod Acartia (Paracartia) grani has been demonstrated to be involved in the life cycle of the parasite infecting flat oysters growing in ponds with a low biodiversity. However, no complex natural environments, such as bays or estuaries, have been studied for a similar purpose. Therefore, a survey for the presence of the protozoan Marteilia was conducted by PCR on the zooplankton community of a natural ecosystem, the Alfacs and Fangar bays in the Delta de l'Ebre (NW Mediterranean). Identification of zooplankton...
Tipo: Text
Palavras-chave: Zooplankton; Harpaticoida sp.; Oithona sp.; Mollusc parasite; Life cycle; Marteilia.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3050.pdf

The French Polynesian experience (Pearl oyster management, a manual)
Provedor de dados: 5
Autores: Berthe, Franck; Prou, Jean.
French Polynesia is currently the world's largest producer of cultured black pearls with exports worth U5$150 millions annually. The activity has become of prime socioeconomic importance the archipelagos with strong support and control of the government. In the mid-1980s, mass mortality of the black-lipped pearl oyster, Pinctada margarltlfera, has occurred in several atolls and challenged the development of pearl farming activity. Consequent studies and surveys have described several pathological conditions. None of those is considered to pose significant threat to the industry. However, they are monitored by a surveillance program in order to prevent and control emerging diseases. The government of French Polynesia has developed efforts to maintain high...
Tipo: Text
Palavras-chave: Health management; Pinctada margaritifera; French Polynesia; Pathogens; Mass mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3703.PDF

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Infection dynamics of Marteilia refringens in flat oyster Ostrea edulis and copepod Paracartia grani in a claire pond of Marennes-Oleron Bay
Provedor de dados: 5
Autores: Audemard, Corinne; Sajus, Marie-céline; Barnaud, Antoine; Sautour, Benoît; Sauriau, Pierre-guy; Berthe, Franck.
The protozoan parasite Marteilia refringens has been partly responsible for the severe decrease in the production of the European flat oyster Ostrea edulis Linnaeus in France since the 1970s. The calanoid copepod Paracartia grani Sars was recently found to be a host for M refringens in French shallow-water oyster ponds ('claires'). This study reconsidered M refringens transmission dynamics in the light of this finding, taking into account not only oyster infection dynamics and environmental factors but also data concerning the copepod host. P. grani population dynamics in the claire under study revealed that this species is the dominant planktonic copepod in this confined ecosystem. During winter, M refringens overwintered in O. edulis, with P. grani...
Tipo: Text
Palavras-chave: Claire pond; Infection dynamics; Paracartia grani; Ostrea edulis; Marteilia refringens.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2858.pdf

Histological abnormalities related to mortality in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia
Provedor de dados: 5
Autores: Berthe, Franck; Legroumellec, Marc; Haffner, Philippe; Renault, Tristan.
Outbreaks of mass mortality occurred since 1993 in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia. Since then, the disease, called "syndrome 93" (S93) has inflicted serious losses (60 to 100%) during the cold period and thus must be considered as an economically significant disease.
Tipo: Text
Palavras-chave: Penaeus stylirostris; Penaeid shrimp; Mortality; Histology.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2974.pdf

Experimental transmission of Marteilia refringens with special consideration of its life cycle
Provedor de dados: 5
Autores: Berthe, Franck; Pernas, Maria; Zerabib, Maamar; Haffner, Philippe; Thebault, Anne; Figueras, Antonio.
Ever since its first description the paramyxean parasite Marteilia refringens has been recognised as one of the most significant pathogens of bivalve molluscs. The existence of a complex life cycle has been postulated by many authors. However, the transmission route of M. refringens and its life cycle remain unresolved. Experimental observations are presented here that may help lead to a satisfactory hypothesis of the M. refringens life cycle. Trials were performed to test the infectivity of purified early and late stages of M. refringens by different routes of inoculation. Horizontal transmission was also tested by cohabitation with carriers in experimental tanks. Field cohabitation and exposure to natural sources of the parasite helped examine the role...
Tipo: Text
Palavras-chave: Marteilia refringens; Ostrea edulis; Transmission trial.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-2742.pdf

Etude des agents pathogènes et épidémiologie
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Robert, Maeva; Chollet, Bruno; Gerard, Andre.
Le programme 4 portant sur la pathologie des coquillages dans le cadre d'une gestion durable des productions ostréicoles, comporte 2 actions principales réparties en une étude d'épidémiologie analytique et descriptive des agents pathogènes (Action 1), et l'étude des relations hôtes/agents pathogènes (Action 2). Les résultats 2001 présentés ici sont relatifs à ces 2 actions, avec notamment des informations sur les infections à virus de type herpès, et de recherche de bactéries pathogènes chez Crassostrea gigas lors des épisodes de mortalités estivales. Par ailleurs, l'émergence d'un programme national, intitulé MOREST (pour Mortalités Estivales) sur l' huître creuse C. gigas va permettre de rappeler le contexte scientifique des relations hôtes/pathogènes.
Tipo: Text
Palavras-chave: Pathologie; Agents pathogènes; Epidémiologie; Huître creuse; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31011/29414.pdf

Traceability of aquatic animals
Provedor de dados: 5
Autores: Hastein, T; Hill, Bj; Berthe, Franck; Lightner, Dv.
Effective methods of traceability are urgently required for use in research as weil as in different types of aquaculture operations and to control trade in aquatic animais and praducts. In regard to the marking of fish, many different tagging methods have been described and the method to be used depends on the purpose and need for tagging. In contrast, for molluscs and crustaceans, only a few methods of marking such animais have been described, due to the practical difficulties. The authors first describe the different methods for tracing fish and fishery products, by means of external tags, such as Floy tags, Carlin tags and passive integrated transponder tags; chemical marking using inorganic substances su ch as silver nitrate or potassium nitrate,...
Tipo: Text
Palavras-chave: Aquatic animals; Crustacea; Fish; Genetic markers; Molluscs; Tags; Traceability.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-2701.pdf

Validation of in situ hybridisation and histology assays for the detection of the oyster parasite Marteilia refringens
Provedor de dados: 5
Autores: Thebault, Anne; Bergman, S; Pouillot, R; Le Roux, Frédérique; Berthe, Franck.
An in situ hybridisation technique has been developed for the detection of infection in oysters with Marteilia refringens with particular emphasis on light infections or confirmation of suspected cases by means of histology. Although validation of new diagnostic methods is usually achieved by comparison with standard techniques, in our case the sensitivity and specificity of the standard (histology) had not previously been established. Another point to consider is that surveillance and monitoring frequently target populations displaying different levels of prevalence under different field conditions. The objective of our study was to evaluate the sensitivity and specificity values of in situ hybridisation and histology for the detection of M refringens,...
Tipo: Text
Palavras-chave: Ostrea edulis; Marteilia refringens; Latent models; Specificity; Sensitivity; Validation; Diagnostic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3624.pdf

Viral gametocytic hypertrophy of Crassostrea gigas in France: from occasional records to disease emergence?
Provedor de dados: 5
Autores: Garcia, Celine; Robert, Maeva; Arzul, Isabelle; Chollet, Bruno; Joly, Jean-pierre; Miossec, Laurence; Comtet, Thierry; Berthe, Franck.
Viral gametocytic hypertrophy was reported for the first time in 2001 in Pacific oyster Crassostrea gigas in France. Since this date, the number of reported cases and the distribution area have increased every year; however, the cases are not associated with macroscopic signs or increased mortality rates. Both male and female gametes were hypertrophied and basophilic inclusions were observed in gamete nuclei, Transmission electron microscopy revealed the presence of viral particles in these intranuclear basophilic inclusions. These particles had characteristics similar to those of the Papillomaviridae and Polyomaviridae families: they were small, non-enveloped, icosahedral, and 44 to 56 nm in diameter, The viral particles were found in male, female and...
Tipo: Text
Palavras-chave: Polyomaviridae; Papillomaviridae; Gonad; Viral garnetocytic hypertrophy; Pacific oyster; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-3288.pdf

In situ hybridization detection of initial infective stages of Marteilia refringens (Paramyxea) in its host Mytilus galloprovincialis
Provedor de dados: 5
Autores: Carrasco, N; Arzul, Isabelle; Berthe, Franck; Furones, M.
Tipo: Text
Palavras-chave: Mussel; Marteilia refringens; In situ hybridization; Gills; Early stages; Connective tissue.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3941.pdf

La réglementation zoosanitaire et son application à la conchyliculture
Provedor de dados: 5
Autores: Berthe, Franck; Thebault, Anne; Grizel, Henri.
As for other animal production sectors, shellfish farming has been afflicted with various diseases and some constitute limiting factors for the quality or the quantity of production.
Tipo: Text
Palavras-chave: Réglementation zoosanitaire; Conchyliculture; Pathologie.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3246.pdf

Réseau de veille zoosanitaire des mollusques REPAMO - Rapport d'activité pour l'année 1997
Provedor de dados: 5
Autores: Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique.
Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf

Bilans depuis 1999 et évolution de la stratégie du REPAMO
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Pichot, Yves; Chollet, Bruno; Robert, Maeva; Le Coguic, Yvette; Tige, Gilbert; Hirata, Toshio; Mazurie, Joseph; Le Saux, Jean-claude; Masson, Nadine; Auby, Isabelle; Maurer, Daniele; Le Roux, Frédérique; Cochennec, Nathalie; Renault, Tristan; Berthe, Franck; Grizel, Henri.
The main objectives of the Mollusc Pathology Network (REPAMO) are to prevent introductions or outbreaks of infectious agents, prevent spread within a farm and especially from one farm to another, to study ways of decreasing the impact of infectious agent and to monitor the development of these pathogens in order to guarantee the health status of oysters exchanged with our commercial partners. On the whole, REPAMO's activities have not changed and include: - monitoring the development of diseases that have to be reported to authorities and carrying out analyses to classify zones: bonamiosis and marteiliosis; - baseline monitoring of farmed and wild populations of bivalve molluscs; - studying cases of abnormal mortality events; - controlling mollusc...
Tipo: Text
Palavras-chave: Mollusques; Réseau surveillance; Pathologie; REPAMO.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3265.pdf

Certifying the French population of Crassostrea gigas free from exotic diseases : a risk analysis approach
Provedor de dados: 5
Autores: Thebault, Anne; Berthe, Franck; Audige, L.
Sample-size calculation in the context of the surveys aimed at substantiating freedom from infection have been commonly undertaken an terrestrial animals over recent years, but not on aquatic animals. A recent model developed by Audigé and Beckett in 1999 can be used to plan and assess animal health surveys. The aim of this study was to adapt that model for marine aquaculture, in particular to help in planning surveys aimed at substantiating freedom form two exotic diseases, mikrocytosis and perkinsosis, in the French population of Crassostrea gigas. As a first approach, farmed animals are so frequent that it would be very difficult to be representative of a single area or zone. To find the most appropriate sampling scheme, the model was run using @Risk...
Tipo: Text
Palavras-chave: Stochastic modelling; Sensitivity modelling; Risk analysis; Oysters; Molluscs; Crassostrea gigas; Certification; Aquatic animal surveys.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-2710.pdf

Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast
Provedor de dados: 5
Autores: Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene.
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A...
Tipo: Text
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf

Suivi bactérien d'un élevage larvaire de crevettes péneïdes - Ecloserie CAMCAR, 12 janvier - 12 février 1991
Provedor de dados: 5
Autores: Haffner, Philippe; Berthe, Franck.
La mission effectuée de mi-janvier à mi-février à l'écloserie CAMCAR (Colombie), avait pour objectif l'analyse quantitative et qualitative de la flore bactérienne présente en élevage larvaire de crevettes pénéides. L'intérêt principal du choix de ce site est de travailler dans une écloserie jeune (- de de deux ans) et n'ayant jamais rencontré de problème pathologique. Cette analyse fait donc le point sur les équilibres bactériens présents dans une écloserie industrielle saine mais bien évidemment dans un lieu et à une époque précise.
Tipo: Text
Palavras-chave: Ecloserie; Bactériologie; Flore bactérienne; Colombie.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00131/24192/22186.pdf

A long term study of bonamiosis in Quiberon bay, France
Provedor de dados: 5
Autores: Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf

Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf