Consulta: creatorFacets:"Bertoni, Marine"
Registros recuperados: 2
Data/hora: 12/06/2024 12:36:05

Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents
Provedor de dados: 5
Autores: Segura, Audrey; Bertoni, Marine; Auffret, Pauline; Klopp, Christophe; Bouchez, Olivier; Genthon, Clemence; Durand, Alexandra; Bertin, Yolande; Forano, Evelyne.
Background The cattle gastrointestinal tract (GIT) is the main enterohemorrhagic Escherichia coli (EHEC) reservoir. In order to identify nutrients required for the survival or multiplication of EHEC in the bovine GIT, we compared the transcriptomes of the EHEC O157:H7 reference strain EDL933 cultured in vitro in bovine digestive contents (DCs) (rumen, small intestine and rectum) using RNA-sequencing. Results Gene expression profiles showed that EHEC EDL933 activated common but also specific metabolic pathways to survive in the different bovine DCs. Mucus-derived carbohydrates seem important in EHEC nutrition in posterior DCs (small intestine and rectum) but not in rumen content. Additional carbohydrates (xylose, ribose, mannitol, galactitol) as well as...
Tipo: Text
Palavras-chave: EHEC O157:H7; Bovine gastrointestinal tract; RNA-seq; Metabolism; Carbon sources; Mucus-derived carbohydrates; Ethanolamine; Respiration.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57563/59734.pdf

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
Provedor de dados: 5
Autores: Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf