Consulta: creatorFacets:"Bidault, Adeline"
Registros recuperados: 13
Data/hora: 01/06/2024 08:12:35

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks
Provedor de dados: 5
Autores: Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine.
In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was...
Tipo: Text
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf

External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease
Provedor de dados: 5
Autores: Rahmani, Alexandra; Mathien, Clémentine; Bidault, Adeline; Le Goïc, Nelly; Paillard, Christine; Pichereau, Vianney.
Aims Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis. Methods and Results In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids...
Tipo: Text
Palavras-chave: BRD; Modulation effect; Neutralizing activity; PH; PH modifications; Ruditapes philippinarum; Vibrio tapetis.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74142/73669.pdf

Development of a Taqman real-time PCR assay for rapid detection and quantification of Vibrio tapetis in extrapallial fluids of clams
Provedor de dados: 5
Autores: Bidault, Adeline; Richard, Gaelle G.; Le Bris, Cedric; Paillard, Christine.
The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600(T) V. tapetis strain. Quantification curves of...
Tipo: Text
Palavras-chave: Vibrio tapetis; VirB4 gene; Taqman real-time PCR; Molecular diagnostic; Venerupis philippinarum; Marine pathogen; Brown ring disease.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00308/41909/73194.pdf

Rapid and efficient protocol to introduce exogenous DNA in Vibrio harveyi and Pseudoalteromonas sp.
Provedor de dados: 5
Autores: Delavat, Francois; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine.
Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudoalteromonas strains. Moreover, a mini-Tn7 delivery system was modified to site-specifically integrate mini-transposons in the genome of V. harveyi ORM4. As a proof-of-principle, replicative plasmids carrying bioreporters were introduced by electroporation in V. harveyi ORM4 cells, and gene expression was followed at the single cell level. We could demonstrate that...
Tipo: Text
Palavras-chave: Electroporation Mini-transposon Vibrio harveyi Pseudoalteromonas; Phenotypic heterogeneity Bioreporter Flagellum expression.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00459/57035/58934.pdf

Identification of Polyunsaturated Fatty Acids Synthesis Pathways in the Toxic Dinophyte Alexandrium minutum Using 13C-Labelling
Provedor de dados: 5
Autores: Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe.
The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched...
Tipo: Text
Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf

Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development
Provedor de dados: 5
Autores: Rahmani, Alexandra; Corre, Erwan; Richard, Gaëlle; Bidault, Adeline; Lambert, Christophe; Oliveira, Louisi; Thompson, Cristiane; Thompson, Fabiano; Pichereau, Vianney; Paillard, Christine.
The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a...
Tipo: Text
Palavras-chave: Brown ring disease; V. tapetis; R. philippinarum; Hemocytes; Actin cytoskeleton; Beta-Thymosin; Coactosin; Resting cells.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00510/62131/66362.pdf

Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Boullot, Floriane; Castrec, Justine; Bidault, Adeline; Dantas, Natanael; Payton, Laura; Perrigault, Mickael; Tran, Damien; Amzil, Zouher; Boudry, Pierre; Soudant, Philippe; Hegaret, Helene; Fabioux, Caroline.
Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sodium channel; Alternative splicing; Alexandrium minutum; Paralytic shellfish toxins.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00368/47877/47892.pdf

Marinilactibacillus piezotolerans sp nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough
Provedor de dados: 5
Autores: Toffin, Laurent; Zink, Klaus; Kato, Chiaki; Pignet, Patricia; Bidault, Adeline; Bienvenu, Nadège; Birrien, Jean-louis; Prieur, Daniel.
A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20(T)) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaGl concentration range for growth was 0-120 gl(-1), with the optimum at 10-20 g l(-1). The temperature range for growth at PH 7.0 was 4-50 degreesC, with the optimum at 37-40 degreesC. The optimum pH for growth was 7.0-8.0. The optimum pressure for growth was 0.1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %);...
Tipo: Text
Palavras-chave: Phylogeny; Biochemistry; Microbiology; Thermophilic bacteria; Deep sub seafloor sediment.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-592.pdf

Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough
Provedor de dados: 5
Autores: Toffin, Laurent; Bidault, Adeline; Pignet, Patricia; Tindall, Brian; Slobodkin, Alexander; Kato, Chiaki; Prieur, Daniel.
A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the...
Tipo: Text
Palavras-chave: Phylogeny; Bacteria; Microbiology; Deep sediment layer.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-590.pdf

Dynamics of the Pacific Oyster Pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy
Provedor de dados: 5
Autores: Lasa, Aide; Cesare, Andrea; Tassistro, Giovanni; Borello, Alessio; Gualdi, Stefano; Furones, Dolors; Carrasco, Noelia; Cheslett, Deborah; Brechon, Amanda; Paillard, Christine; Bidault, Adeline; Pernet, Fabrice; Canesi, Laura; Edomi, Paolo; Pallavicini, Alberto; Pruzzo, Carla; Vezzulli, Luigi.
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 (OsHV‐1) have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve disease. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00508/61952/66036.pdf

Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs
Provedor de dados: 5
Autores: Dias, Graciela M.; Bidault, Adeline; Le Chevalier, Patrick; Choquet, Gwenaelle; Sarkissian, Clio Der; Orlando, Ludovic; Medigue, Claudine; Barbe, Valerie; Mangenot, Sophie; Thompson, Cristiane C.; Thompson, Fabiano L.; Jacq, Annick; Pichereau, Vianney; Paillard, Christine.
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan-and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I...
Tipo: Text
Palavras-chave: Vibrio tapetis; Venerupis philippinarum; Comparative genomics; Pathogenicity; T4SS; Pangenome; Core genome.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00429/54077/72091.pdf

GenoPopTaille. Moving stock assessment into the future
Provedor de dados: 5
Autores: Marandel, Florianne; Biais, Gerard; Bidault, Adeline; Charrier, Grégory; Cornette, Florence; Lapegue, Sylvie; Laroche, Jean; Le Cam, Sabrina; Stephan, Eric; Trenkel, Verena; Lorance, Pascal.
At the ecosystem level, sustainable exploitation of fisheries resources depends not only on the status of target species but also on that of bycatch species, some of which are even more sensitive to exploitation. This is the case for a number of elasmobranchs (skates, rays and sharks) species whose abundance declined during the 20th century. Further, the biology of elamobranchs is still poorly known and traditional fisheries stock assessment methods using fisheries catches and scientific survey data for estimating abundance are expensive or even inapplicable due to the small numbers observed. The GenoPopTaille project attempts to apply to the case of the thornback ray (Raja clavata) recent genetic-based methods for absolute population abundance estimation...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00343/45439/44960.pdf

The marine intertidal zone shapes oyster and clam digestive bacterial microbiota
Provedor de dados: 5
Autores: Offret, Clement; Paulino, Sauvann; Gauthier, Olivier; Château, Kevin; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Pernet, Fabrice; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaelle Le.
Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position....
Tipo: Text
Palavras-chave: Microbiota; Oysters; Clams; Intertidal zone; Digestive gland; Metabarcoding.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00628/73961/73320.pdf