Consulta: creatorFacets:"Bierne, Nicolas"
Registros recuperados: 33
Data/hora: 06/06/2024 12:14:03

Fragmentation des populations naturelles d'Ostrea edulis: une adaptation locale de l'huître plate européenne
Provedor de dados: 5
Autores: Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique.  La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf

Comprendre et inférer la connectivité marine pour mieux gérer les ressources
Provedor de dados: 5
Autores: Bierne, Nicolas; Arnaud-haond, Sophie.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59820/62963.pdf

Variation in synonymous codon use and DNA polymorphism within the Drosophila genome
Provedor de dados: 5
Autores: Bierne, Nicolas; Eyre Walker, A.
A strong negative correlation between the rate of amino-acid substitution and codon usage bias in Drosophila has been attributed to interference between positive selection at nonsynonymous sites and weak selection on codon usage. To further explore this possibility we have investigated polymorphism and divergence at three kinds of sites: synonymous, nonsynonymous and intronic in relation to codon bias in D. melanogaster and D. simulans. We confirmed that protein evolution is one of the main explicative parameters for interlocus codon bias variation (r(2) similar to 40%). However, intron or synonymous diversities, which could have been expected to be good indicators of local interference [here defined as the additional increase of drift due to selection on...
Tipo: Text
Palavras-chave: Substitution rate; Selective constraints; Linkage; Hill Robertson interference; GC content; Drosophila; Diversity; Codon usage.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-4221.pdf

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe
Provedor de dados: 5
Autores: Yonemitsu, Marisa A; Giersch, Rachael M; Polo-prieto, Maria; Hammel, Maurine; Simon, Alexis; Cremonte, Florencia; Avilés, Fernando T; Merino-véliz, Nicolás; Burioli, Erika Av; Muttray, Annette F; Sherry, James; Reinisch, Carol; Baldwin, Susan A; Goff, Stephen P; Houssin, Maryline; Arriagada, Gloria; Vázquez, Nuria; Bierne, Nicolas; Metzger, Michael J.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00590/70255/68304.pdf

Speciation in the Deep Sea: Multi-Locus Analysis of Divergence and Gene Flow between Two Hybridizing Species of Hydrothermal Vent Mussels
Provedor de dados: 5
Autores: Faure, Baptiste; Jollivet, Didier; Tanguy, Arnaud; Bonhomme, Francois; Bierne, Nicolas.
Background: Reconstructing the history of divergence and gene flow between closely-related organisms has long been a difficult task of evolutionary genetics. Recently, new approaches based on the coalescence theory have been developed to test the existence of gene flow during the process of divergence. The deep sea is a motivating place to apply these new approaches. Differentiation by adaptation can be driven by the heterogeneity of the hydrothermal environment while populations should not have been strongly perturbed by climatic oscillations, the main cause of geographic isolation at the surface. Methodology/Principal Finding: Samples of DNA sequences were obtained for seven nuclear loci and a mitochondrial locus in order to conduct a multi-locus...
Tipo: Text
Palavras-chave: Mid atlantic ridge; Snake pit area; Population genetics; Drosophila pseudooscura; Bathymodiolus bivalvia; Statistical properties; Subdivided population; Phylogenetic networks; Dna polymorphism; Neutrality tests.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00129/24012/21972.pdf

Fitness landscapes support the dominance theory of post-zygotic isolation in the mussels Mytilus edulis and M. galloprovincialis
Provedor de dados: 5
Autores: Bierne, Nicolas; Bonhomme, Francois; Boudry, Pierre; Szulkin, M; David, Pierre.
We studied the genetic basis of post-zygotic isolation in the marine mussels Mytilus edulis and Mytilus galloprovincialis. Evidence was obtained for a high number of recessive Dobzhansky-Muller substitutions in the genome of these two mussel taxa. We analysed the segregation of unlinked diagnostic markers in the progeny of two backcrosses and an F-2 cross, 36 h and 200 days after fertilization. Directional selection favouring M. galloprovincialis genotypes was observed in both kinds of cross. In the F-2, epistatic interactions between each pair of chromosome fragments mapped by the markers were identified in addition. Our results imply that homozygous-homozygous interactions are required for breakdown of coadaptation, in accordance with the dominance...
Tipo: Text
Palavras-chave: Mytilus; Dobzhansky Muller incopatibilities; Post zygotic isolation; Negative epistasis; Hybrid breakdown; Hybrid zone.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1350.pdf

Using neutral, selected, and hitchhiker loci to assess connectivity of marine populations in the genomic era
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Broquet, Thomas; Aurelle, Didier; Viard, Frederique; Souissi, Ahmed; Bonhomme, Francois; Arnaud-haond, Sophie; Bierne, Nicolas.
Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known...
Tipo: Text
Palavras-chave: Connectivity; Gene flow; Marine conservation; Population genomics; Population structure.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00275/38592/37120.pdf

Relationships between genetic diversity and niche-partitioning of Synechococcus populations in Mediterranean coastal lagoons, systems of transition between freshwater and marine ecosystems
Provedor de dados: 5
Autores: Masseret, Estelle; Bierne, Nicolas; Chevret, Pascale; Roumagnac, Marie; Bec, Béatrice; Laugier, Thierry; Collos, Yves; Vaquer, Andre.
Coastal lagoons are morphologically and ecologically complex ecosystems, subject to constantly changing environmental conditions generally of much greater magnitude than it is recorded in the open sea. Due to their transitional nature, they usually display a number of specific features (ie. high nutrient concentrations, light spectral quality, salinity range) which are potentially influencing strategies of adaptation of photosynthetic microorganisms. The unicellular cyanobacteria Synechococcus is a major component of marine and freshwater picophytoplankton. Its genetic diversity is well documented for these ecosystems, but knowledge about biodiversity and distribution of this genus in transitional waters remains scarce. The phylogenetic analysis of a...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00011/12198/8960.pdf

Can habitat specialisation maintain a mosaic hybrid zone in marine bivalves?
Provedor de dados: 5
Autores: Bierne, Nicolas; David, Pierre; Langlade, Aime; Bonhomme, F.
Maintaining the integrity of differentiated genomes in marine organisms needs efficient isolation mechanisms, because planktonic larval dispersion provides contacts between taxa. Habitat specialisation is interesting in this respect, because it can both prevent interspecific crosses (each taxon reproduces in its own habitat) and eliminate hybrids (typically less fit than a parental taxon in each habitat). The contact zone between smooth-shelled mussels Mytilus edulis and M. galloprovincialis in Europe is a good example, as allozyme genotypes typical of both taxa seem to segregate into different habitats. However, allozymes may be selected directly and it is not known whether the same pattern can be extended to the whole genome. Here, we used 6 presumably...
Tipo: Text
Palavras-chave: Habitat specialisation; Hybrid zone; Introgression; Mytilus edulis; Mytilus galloprovincialis.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00000/10228/7597.pdf

A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction
Provedor de dados: 5
Autores: Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas.
Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the...
Tipo: Text
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf

The origin and remolding of genomic islands of differentiation in the European sea bass
Provedor de dados: 5
Autores: Duranton, Maud; Allal, Francois; Fraisse, Christelle; Bierne, Nicolas; Bonhomme, Francois; Gagnaire, Pierre-alexandre.
Speciation is a complex process that leads to the progressive establishment of reproductive isolation barriers between diverging populations. Genome-wide comparisons between closely related species have revealed the existence of heterogeneous divergence patterns, dominated by genomic islands of increased divergence supposed to contain reproductive isolation loci. However, this divergence landscape only provides a static picture of the dynamic process of speciation, during which confounding mechanisms unrelated to speciation can interfere. Here we use haplotype-resolved whole-genome sequences to identify the mechanisms responsible for the formation of genomic islands between Atlantic and Mediterranean sea bass lineages. Local ancestry patterns show that...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00446/55750/57409.pdf

Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).  Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf

Mark-recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids
Provedor de dados: 5
Autores: Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P.
We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream...
Tipo: Text
Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf

Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe.  Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60).  Results A female-biased sex-ratio and a higher condition index were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf

Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf

Mortalités de moules bleues dans les secteurs mytilicoles : description et étude des facteurs liés, action –MORBLEU-2017
Provedor de dados: 5
Autores: Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Costes, Louis; Degremont, Lionel; Garcia, Celine; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Morga, Benjamin; Normand, Julien; Robert, Stephane; Saunier, Alice; Soletchnik, Patrick; Travers, Marie-agnes.
Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015 et 2016 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2017, les trois objectifs principaux étaient de : i) poursuivre la description du phénomène de mortalité ainsi que le contexte environnemental associé en effectuant une nouvelle campagne d’études et d’observations in situ, ii) tester des hypothèses...
Tipo: Text
Palavras-chave: Moules; Mytilus edulis; Mortalité; Statut cytogénétique; Anomalies génomiques; Populations; Facteurs environnementaux; Ecologie microbienne.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00503/61503/65337.pdf

Assortative fertilization and selection at larval stage in the mussels Mytilus edulis and M-galloprovincialis
Provedor de dados: 5
Autores: Bierne, Nicolas; David, Pierre; Boudry, Pierre; Bonhomme, F.
Assortative mating (prezygotic isolation) and reduced hybrid fitness (postzygotic isolation) are typically invoked to explain the stability of hybrid zones. In the tension zone model. these factors work in opposition to migration, which promotes genetic homogeneity. Many marine animals migrate over long distances through a planktonic larval stage. Therefore. strong reproductive isolation is needed to maintain stable marine hybrid zones. However, surprisingly little is known about mating preferences and hybrid fitness in marine organisms. Smooth-shelled mussels (Mytilus spp.) form a well-known species complex, with hybridization over extensive areas such as the contact zone of M. edulis and M. galloprovincialis around European Atlantic coasts. This paper...
Tipo: Text
Palavras-chave: Preferential fertilization; Mytilus galloprovincialis; Mytilus edulis; Hybrid viability; Heterosis; Gamete competition.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2781.pdf

Replicated anthropogenic hybridisations reveal parallel patterns of admixture in marine mussels
Provedor de dados: 5
Autores: Simon, Alexis; Arbiol, Christine; Nielsen, Einar Eg; Couteau, Jérôme; Sussarellu, Rossana; Burgeot, Thierry; Bernard, Ismaël; Coolen, Joop W. P.; Lamy, Jean-baptiste; Robert, Stephane; Skazina, Maria; Strelkov, Petr; Queiroga, Henrique; Cancio, Ibon; Welch, John J.; Viard, Frédérique; Bierne, Nicolas.
Human‐mediated transport creates secondary contacts between genetically differentiated lineages, bringing new opportunities for gene exchange. When similar introductions occur in different places, they provide informally replicated experiments for studying hybridisation. We here examined 4,279 Mytilus mussels, sampled in Europe and genotyped with 77 ancestry‐informative markers. We identified a type of introduced mussels, called “dock mussels,” associated with port habitats and displaying a particular genetic signal of admixture between M. edulis and the Mediterranean lineage of M. galloprovincialis. These mussels exhibit similarities in their ancestry compositions, regardless of the local native genetic backgrounds and the distance separating colonised...
Tipo: Text
Palavras-chave: Admixture; Bentho-pelagic species; Biological introductions; Clines; Ports; Secondary contact.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70600/68791.pdf

Within-Generation Polygenic Selection Shapes Fitness-Related Traits across Environments in Juvenile Sea Bream
Provedor de dados: 5
Autores: Rey, Carine; Darnaude, Audrey; Ferraton, Franck; Guinand, Bruno; Bonhomme, François; Bierne, Nicolas; Gagnaire, Pierre-alexandre.
Understanding the genetic underpinnings of fitness trade-o s across spatially variable environments remains a major challenge in evolutionary biology. In Mediterranean gilthead sea bream, first-year juveniles use various marine and brackish lagoon nursery habitats characterized by a trade-o between food availability and environmental disturbance. Phenotypic di erences among juveniles foraging in di erent habitats rapidly appear after larval settlement, but the relative role of local selection and plasticity in phenotypic variation remains unclear. Here, we combine phenotypic and genetic data to address this question. We first report correlations of opposite signs between growth and condition depending on juvenile habitat type. Then, we use single...
Tipo: Text
Palavras-chave: Antagonistic pleiotropy; Habitat association; Fitness trade-off; Juvenile growth; Polygenic scores; RAD-sequencing; Spatially varying selection.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00622/73370/72560.pdf

Dedicated population genomics for the silent world: the specific questions of marine population genetics
Provedor de dados: 5
Autores: Bierne, Nicolas; Bonhomme, Francois; Arnaud-haond, Sophie.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00374/48545/48841.pdf

Population genomics shed light on the demographic and adaptive histories of European invasion in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Crassostrea gigas originated from the Pacific coast of Asia, but was introduced into several European countries in the early 1970s. Natural populations have now spread across the length of the western seaboard of Europe. To elucidate the demographic and selective processes at play during this rapid expansion, genome-scan analysis was performed on different populations. High diversities and low differentiation were observed overall, but significant genetic differentiation was found among newly established populations and between the newly established northern group and a nearly panmictic group composed of southern European populations and a population from Japan. Loss of genetic diversity was also seen in the north, likely caused by founder events during...
Tipo: Text
Palavras-chave: AFLPs; Crassostrea gigas; Genome scan; Invasive species; Microsatellites; SNPs.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/25977/24070.pdf

Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Huvet, Arnaud; Balabaud, K; Bierne, Nicolas; Boudry, Pierre.
Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas...
Tipo: Text
Palavras-chave: Paternal contribution; Microsatellites; In vitro crossings; Hybridization; Crassostrea angulata; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-436.pdf

Exploring local reproductive differentiation of Pacific oyster population recently settled in northern Europe by transcriptomic approach
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lelong, Christophe; Pernet, Fabrice; Quillen, Virgile; Lapegue, Sylvie; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Originating from north-eastern Asia, the Pacific oyster Crassostrea gigas has been introduced, mainly for aquaculture purpose, into a large number of countries. Although highly variable, its invasive nature has been reported in an increasing number of coastal areas, notably. Northern Europe. We set up a common garden experiment based on the comparison of progenies of Pacific oysters sampled in France, where populations settled since the introduction of the species in the 70’s, and Denmark, where established populations were observed since the beginning of 90s. Pure and hybrid progenies, resulting from crosses within and between adults sampled in both populations, were studied for their sex-ratio, condition index, transcriptomics, and biochemical parameters
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27815/26008.pdf

Application of high-throughput sequencing to population differentiation in the cupped oysters Crassostrea angulata/C. gigas
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie.
The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf

Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf

"Bivalvomix" : génomique évolutive des bivalves marins
Provedor de dados: 5
Autores: Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang &amp; Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf

Large-scale genetic panmixia in the blue shark (     Prionace glauca     ): a single worldwide population, or a genetic lag-time effect of the “grey zone” of differentiation?
Provedor de dados: 5
Autores: Bailleul, Diane; Mackenzie, Alicia; Sacchi, Olivier; Poisson, Francois; Bierne, Nicolas; Arnaud-haond, Sophie.
The blue shark Prionace glauca, among the most common and widely studied pelagic sharks, is a top predator, exhibiting the widest distribution range. However, little is known about its population structure and spatial dynamics. With an estimated removal of 10 to 20 million individuals per year by fisheries, the species is classified as “Near Threatened” by International Union for Conservation of Nature. We lack the knowledge to forecast the long-term consequences of such a huge removal on this top predator itself and on its trophic network. The genetic analysis of more than 200 samples collected at broad scale (from Mediterranean Sea, North Atlantic and Pacific Oceans) using mtDNA and nine microsatellite markers allowed to detect signatures of genetic...
Tipo: Text
Palavras-chave: Blue shark; Conservation; Fisheries; Genetic panmixia; Prionace glauca; Stock.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00417/52866/53776.pdf

A New Barrier to Dispersal Trapped Old Genetic Clines That Escaped the Easter Microplate Tension Zone of the Pacific Vent Mussels
Provedor de dados: 5
Autores: Plouviez, Sophie; Faure, Baptiste; Le Guen, Dominique; Lallier, Francois; Bierne, Nicolas; Jollivet, Didier.
Comparative phylogeography of deep-sea hydrothermal vent species has uncovered several genetic breaks between populations inhabiting northern and southern latitudes of the East Pacific Rise. However, the geographic width and position of genetic clines are variable among species. In this report, we further characterize the position and strength of barriers to gene flow between populations of the deep-sea vent mussel Bathymodiolus thermophilus. Eight allozyme loci and DNA sequences of four nuclear genes were added to previously published sequences of the cytochrome c oxidase subunit I gene. Our data confirm the presence of two barriers to gene flow, one located at the Easter Microplate (between 21 degrees 33'S and 31 degrees S) recently described as a hybrid...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00185/29622/28006.pdf

Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Lapegue, Sylvie; Bierne, Nicolas; Boudry, Pierre.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the...
Tipo: Text
Palavras-chave: QTLs analyses; Summer mortaliy; Genetic; SNPs; Crassostrea gigas; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3351.pdf

Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf

Mortalités de moules bleues dans les secteurs mytilicoles : description et étude des facteurs liés, action – MORBLEU-2018
Provedor de dados: 5
Autores: Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Chasselin, Leo; Degremont, Lionel; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Normand, Julien; Robert, Stephane; Soletchnik, Patrick.
Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015- 2017 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2018, les actions d’observation et de suivis du milieu et des moules ont été fortement réduites et trois axes principaux ont été poursuivis : i) l’étude de la relation entre l’évolution des équilibres microbiens planctoniques et l’apparition de mortalités...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00588/70056/67999.pdf

Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris
Provedor de dados: 5
Autores: Bierne, Nicolas; Bezuart, Ivan; Vonau, Vincent; Bonhomme, François; Bedier, Edouard.
Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the...
Tipo: Text
Palavras-chave: Penaeus stylirostris; Heterozygosity; Marker associated heterosis; Inbreeding depression; Microsatellites.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-753.pdf

Mortalités de moules bleues dans les secteurs mytilicoles charentais et vendéens : description et facteurs liés – MORBLEU
Provedor de dados: 5
Autores: Pepin, Jean-francois; Benabdelmouna, Abdellah; Degremont, Lionel; Guesdon, Stephane; Le Moine, Olivier; Morga, Benjamin; Bierne, Nicolas; Travers, Marie-agnes; Robert, Stephane; Soletchnik, Patrick.
Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une première année d’étude a été réalisée au travers de l’action MORBLEU (Mortalité des moules Bleues). Elle a été menée dans les Pertuis charentais et la baie de Bourgneuf. Dans ce même contexte, elle a été reconduite en 2016 afin de poursuivre l’analyse des données collectées en 2015, renouveler une campagne d’étude et d’observation in situ, formuler des hypothèses autour de facteurs favorisants ces épisodes de mortalité de moules, développer des essais permettant de tester ces hypothèses dans le futur et produire des lots de moules pour ces essais et pour caractériser le potentiel adaptatif des animaux face aux épisodes de...
Tipo: Text
Palavras-chave: Moules; Mytilus edulis; Facteurs environnementaux; Mortalité; Equilibre microbien; Populations; Qualité cytogénétique; Anomalies hémocytaires; Expression de gènes; Physiologie.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00391/50288/50978.pdf