Consulta: creatorFacets:"Bornet, Benjamin"
Registros recuperados: 2
Data/hora: 11/06/2024 08:08:20

Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters
Provedor de dados: 5
Autores: Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.
Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...
Tipo: Text
Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf

ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton
Provedor de dados: 5
Autores: Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...
Tipo: Text
Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf