Consulta: creatorFacets:"Brancalhão,Rose M.C."
Registros recuperados: 3
Data/hora: 16/06/2024 20:28:25

Nucleopolyhedrovirus: scanning electron microscopy technique
Provedor de dados: 82
Autores: Torquato,Ednéia F.B.; Miranda Neto,Marcílio H. de; Brancalhão,Rose M.C.; Franco,Valdeni S.
A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Bombyx mori; BmMNPV; Polyhedra; Truncated octahedron.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600011

Nucleopolyhedrovirus infected central nervous system cells of Bombyx mori (L.) (Lepidoptera: Bombycidae)
Provedor de dados: 82
Autores: Torquato,Ednéia F.B.; Miranda Neto,Marcilio H. de; Brancalhão,Rose M.C.
BmMNPV, a Nucleopolyhedrovirus isolated from infected Bombyx mori (L.) larvae in Paraná State - Brazil, was used to inoculate healthy 5th-instar B. mori larvae and examine the infection on central nervous system (CNS) cells. Samples of nervous tissue were removed from the infected insects, at different sampling times, and processed for cytopathology studies by light and transmission electron microscopy using routine techniques. The experiment included both inoculated and non-inoculated larvae (control). BmMNPV infection was detected on the 5th day after inoculation in CNS cells. Initially, infection was characterized by nuclear hypertrophy and the presence of virogenic stroma, in which the progeny virions were produced. Virions are enveloped and occluded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect; Silkworm; Baculoviridae; Neural lamella; Perineurium.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000100010

Administering ascorbic acid to rats undergoing ageing processes: effects on myosin-V immunoreactive myenteric neurons
Provedor de dados: 42
Autores: Mello,Sônia A.; Marese,Angélica C.M.; Brancalhão,Rose M.C.; Zanoni,Jacqueline N.; Natali,Maria Raquel M.
During the ageing process the enteric nervous system undergoes morphofunctional changes, such as enteric neurodegeneration. Neuronal death can be attributed to increase radicals free, and ascorbic acid (AA), known antioxidant, could minimize damage cause by oxidative stress. The objective of this study is to analyse the behaviour of morphoquantative myenteric neurons in the duodenum of adult Wistar rats with aged 90 (C90), 345 (E345) and 428 (E428) days, as well as animals of the same age who received ascorbic acid supplementation for 120 days (EA345 and EA428). Whole-mount preparations of muscle layer from the duodenum of the animals were immunostained by the method myosin V. 80 microscopic fields were quantified (14.8 mm2/animal) and measured 100...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Antioxidant; Enteric nervous system; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100337