Consulta: creatorFacets:"Brandão,Paulo Eduardo"
Registros recuperados: 12
Data/hora: 06/06/2024 16:23:10

Could human coronavirus OC43 have co-evolved with early humans?
Provedor de dados: 74
Autores: Brandão,Paulo Eduardo.
Abstract This paper reports on an investigation of the role of codon usage evolution on the suggested bovine-to-human spillover of Bovine coronavirus (BCoV), an enteric/respiratory virus of cattle, resulting in the emergence of the exclusively respiratory Human coronavirus OC43 (HCoV-OC43). Analyses based on full genomes of BCoV and HCoV-OC43 and on both human and bovine mRNAs sequences of cholecystokinin (CCK) and surfactant protein 1 A (SFTP1-A), representing the enteric and respiratory tract codon usage, respectively, have shown natural selection leading to optimization or deoptimization of viral codon usage to the human enteric and respiratory tracts depending on the virus genes under consideration. A higher correlation was found for the nucleotide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Codon usage; Coronavirus; Spillover; Coevolution.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400692

Seroprevalence of antibodies against group A rotavirus in cattle from a pioneer frontier in Brazilian Amazon
Provedor de dados: 62
Autores: Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio.
Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003

Short-interfering RNAs as antivirals against rabies
Provedor de dados: 55
Autores: Brandão,Paulo Eduardo; Castilho,Juliana Galera; Fahl,Willian; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Macedo,Carla Isabel; Carrieri,Maria Luiza; Kotait,Ivanete.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SiRNA; Rabies; Antiviral.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011

Species determination of Brazilian mammals implicated in the epidemiology of rabies based on the control region of mitochondrial DNA
Provedor de dados: 55
Autores: Carnieli Junior,Pedro; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Brandão,Paulo Eduardo; Carrieri,Maria Luiza; Kotait,Ivanete.
Identification of animals that are decomposing or have been run over or burnt and cannot be visually identified is a problem in the surveillance and control of infectious diseases. Many of these animals are wild and represent a valuable source of information for epidemiologic research as they may be carriers of an infectious agent. This article discusses the results obtained using a method for identifying mammals genetically by sequencing their mitochondrial DNA control region. Fourteen species were analyzed and identified. These included the main reservoirs and transmitters of rabies virus, namely, canids, chiroptera and primates. The results prove that this method of genetic identification is both efficient and simple and that it can be used in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Sequencing; Identification; Mammals; Rabies.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600002

Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Provedor de dados: 55
Autores: Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008

A coronavirus detected in the vampire bat Desmodus rotundus
Provedor de dados: 55
Autores: Brandão,Paulo Eduardo; Scheffer,Karin; Villarreal,Laura Yaneth; Achkar,Samira; Oliveira,Rafael de Novaes; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Kotait,Ivanete; Richtzenhain,Leonardo José.
This article reports on the identification of a group 2 coronavirus (BatCoV DR/2007) in a Desmodus rotundus vampire bat in Brazil. Phylogenetic analysis of ORF1b revealed that BatCoV DR/2007 originates from a unique lineage in the archetypical group 2 coronaviruses, as described for bat species elsewhere with putative importance in Public Health.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronavirus; Vampire bat; RNA-dependent; RNA-polymerase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600003

Desmodus rotundus and Artibeus spp. bats might present distinct rabies virus lineages
Provedor de dados: 55
Autores: Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo.
In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008

Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Provedor de dados: 58
Autores: Miotto,Bruno Alonso; Hora,Aline Santana da; Taniwaki,Sueli Akemi; Brandão,Paulo Eduardo; Heinemann,Marcos Bryan; Hagiwara,Mitika Kuribayashi.
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared with those from a well-established conventional PCR targeting the 16S RNA encoding gene associated with nucleotide sequencing analysis. The overall agreement between the assays was 94.8% (confidence interval [CI] 95% 88-100%). The newly developed assay presented 91.6% (CI 95% 71.5-98.5%) relative sensitivity (22[+] lipl32 PCR/24[+] 16S RNA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Dog; Urine; QPCR; Lipl32.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300584

Paralytic rabies in swine
Provedor de dados: 58
Autores: Pessoa,Clarice Ricardo de Macedo; Silva,Maria Luana Cristiny Rodrigues; Gomes,Albério Antônio de Barros; Garcia,Andrea Isabel Estévez; Ito,Fumio Honma; Brandão,Paulo Eduardo; Riet-Correa,Franklin.
Rabies transmitted by vampire bats was diagnosed in pigs with paralysis of the pelvic limbs. Diffuse nonsuppurative encephalomyelitis, affecting mainly the spinal cord, was observed histologically. Despite the various diagnosis of rabies in pigs this is the first report of clinical signs and pathology of rabies transmitted by vampire bats.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vampire bats; Epidemiology; Virus isolation; Zoonotic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100038

Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells
Provedor de dados: 58
Autores: Jerez,José Antonio; Gregori,Fábio; Brandão,Paulo Eduardo; Rodriguez,Cesar Alejandro Rosales; Ito,Fumio Honma; Buzinaro,Maria da Glória; Sakai,Takeo.
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine coronavirus (BCoV); Isolation; HmLu-1 cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001

Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay
Provedor de dados: 58
Autores: Souza,Sibele Pinheiro de; Asano,Karen Miyuki; Sandri,Thaisa Lucas; Barros,Iracema Nunes de; Richtzenhain,Leonardo José; Brandão,Paulo Eduardo.
This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine coronavirus; Restriction enzyme; Genotypes; Gene S.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300034

Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
Provedor de dados: 58
Autores: Favaro,Patricia Filippsen; Fernandes,Wilson Roberto; Reischak,Dilmara; Brandão,Paulo Eduardo; Silva,Sheila Oliveira de Souza; Richtzenhain,Leonardo José.
Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine influenza; H3N8; Hemagglutinin; Neuraminidase; Evolutionary analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336