Consulta: creatorFacets:"Browdy, Craig L."
Registros recuperados: 3
Data/hora: 01/06/2024 03:35:40

Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei
Provedor de dados: 5
Autores: Labreuche, Yannick; Veloso, Artur; De La Vega, Enrique; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.
Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both...
Tipo: Text
Palavras-chave: Shrimp; Litopenaeus vannamei; RNAi; DsRNA; Argonaute; Sid-1; Antiviral immunity.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12652/9587.pdf

Advances in Genomics and Genetics of Penaeid Shrimp
Provedor de dados: 5
Autores: Robalino, Javier; Chapman, Robert W.; De La Vega, Enrique; O'Leary, Nuala A.; Gorbach, Danielle M.; Du, Zhi-qiang; Rothschild, Max F.; Browdy, Craig L.; Warr, Gregory; Labreuche, Yannick.
This chapter contains sections titled:    Introduction    EST Collection as an Approach to Gene Discovery in Shrimp    Medium- to High-Throughput Studies of Differential Expression and Gene Discovery    RNAi-Based Applications in Shrimp Aquaculture: From Reverse Genetics to Control of Diseases    Markers, Genetic Maps, and Large Insert Genomic Libraries in Shrimp    Analytical Challenges in Genomics and Genetics of Shrimp    Concluding Remarks    Acknowledgments    References
Tipo: Text
Palavras-chave: Penaeid shrimp EST collection; Gene discovery gene expression “snapshot” gametogenesis abiotic stress responses differential expression cloning RNAi-based applications; Shrimp targeted gene silencing RNAi; And gene function genetic markers in breeding.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00070/18149/15775.pdf

Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp
Provedor de dados: 5
Autores: Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection...
Tipo: Text
Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf