Consulta: creatorFacets:"Burgeot, Thierry"
Registros recuperados: 62
Data/hora: 06/06/2024 18:02:53

The integrated biomarker response revisited: optimization to avoid misuse
Provedor de dados: 5
Autores: Devin, S.; Burgeot, Thierry; Giamberini, L.; Minguez, L.; Pain-devin, S.
The growing need to evaluate the quality of aquatic ecosystems led to the development of numerous monitoring tools. Among them, the development of biomarker-based procedures, that combine precocity and relevance, is recommended. However, multi-biomarker approaches are often hard to interpret, and produce results that are not easy to integrate in the environmental policies framework. Integrative index have been developed, and one of the most used is the integrated biomarker response (IBR). However, an analysis of available literature demonstrated that the IBR suffers from a frequent misuse and a bias in its calculation. Then, we propose here a new calculation method based on both a more simple formula and a permutation procedure. Together, these...
Tipo: Text
Palavras-chave: Biomarkers; Integrated Index; Environmental risk assessment; Pollution; Water Framework Directive.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00176/28712/27276.pdf

Biological effects of the "Erika" oil spill on the common mussel (Mytilus edulis)
Provedor de dados: 5
Autores: Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois.
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during...
Tipo: Text
Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf

Campagne dédiée à l'évaluation des effets biologiques induits par la contamination chimique en baie de Seine. SELISEINE 2018
Provedor de dados: 5
Autores: Mauffret, Aourell; Wessel, Nathalie; Roubeix, Vincent; Akcha, Farida; Chouvelon, Tiphaine; Aminot, Yann; Munschy, Catherine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Mahe, Kelig; Godfrin, Yoann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Bruzac, Sandrine; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Burgeot, Thierry.
Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Le présent rapport concerne le volet SELIseine. Des poissons plats (194 soles, flets et limandes) ont été prélevés en septembre 2018 sur le N/O Antéa, et des moules ont été prélevées en février 2019 sur 6 stations dans le cadre du suivi ROCCH MV suivant le protocole de la convention OSPAR (2012). Les biomarqueurs suivis chez ces organismes couvrent plusieurs types d’effets toxiques (reprotoxicité, génotoxicité,...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00688/80024/83012.pdf

Relationship between PAH biotransformation as measured by biliary metabolites and EROD activity, and genotoxicity in juveniles of sole (Solea solea)
Provedor de dados: 5
Autores: Wessel, Nathalie; Santos, Raphael; Menard, Dominique; Le Menach, K.; Buchet, Vincent; Le Bayon, Nicolas; Loizeau, Veronique; Burgeot, Thierry; Budzinski, H.; Akcha, Farida.
Polycylic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environment Their toxicity is mainly linked to the ability of marine species to biotransform them into reactive metabolites PAHs are thus often detected at trace levels in animal tissues For biomonitoring purposes this findings have two main consequences (i) the determination of the PAH tissue concentration is not suitable for the evaluation of individual exposure to PAHs (ii) It can explain sometimes the lack of correlations obtained with relevant markers of toxicity such as genotoxicity biomarkers The aim of the present study was to better investigate the link between PAH exposure and genotoxicity in marine flatfish During a laboratory experiment juvenile soles were exposed...
Tipo: Text
Palavras-chave: Ecotoxicology; Sole; PAH; Metabolites; EROD; Comet assay.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12970/10834.pdf

Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes: Screening of 23 molecules using flow cytometry
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan.
The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed...
Tipo: Text
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf

Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Barranger, Audrey; Heude-berthelin, Clothilde; Rouxel, Julien; Adeline, Beatrice; Benabdelmouna, Abdellah; Burgeot, Thierry; Akcha, Farida.
Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oxidative stress; DNA damage; Germ cell; Pesticides.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00295/40655/39753.pdf

Sampling design optimization for EROD measurements in fish
Provedor de dados: 5
Autores: Beliaeff, Benoit; Burgeot, Thierry.
Activity of 7-ethoxyresorufin-O-deethylase (EROD) in fish is certainly the best-studied biomarker of exposure applied in the field to evaluate biological effects of contamination in the marine environment. Since 1991, a feasibility study for a monitoring network using this biomarker of exposure has been conducted along French coasts. Using data obtained during several cruises, this study aims to determine the number of fish required to detect a given difference between 2 mean EROD activities, i.e. to achieve an a priori fixed statistical power (l-beta) given significance level (alpha), variance estimations and projected ratio of unequal sample sizes (k). Mean EROD activity and standard error were estimated at each of 82 sampling stations. The...
Tipo: Text
Palavras-chave: Biomonitoring; Estimation; EROD activity; Statistical power; Sample size; Sampling design.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00337/44787/44483.pdf

Integrated chemical and biological assessment of contaminant impacts in selected european coastal and offshore marine areas
Provedor de dados: 5
Autores: Hylland, Ketil; Robinson, Craig D.; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Mattew J.
This paper reports a full assessment of results from ICON, an international workshop on marine integrated contaminant monitoring, encompassing different matrices (sediment, fish, mussels, gastropods), areas (Iceland, North Sea, Baltic, Wadden Sea, Seine estuary and the western Mediterranean) and endpoints (chemical analyses, biological effects).  ICON has demonstrated the use of a framework for integrated contaminant assessment on European coastal and offshore areas. The assessment showed that chemical contamination did not always correspond with biological effects, indicating that both are required. The framework can be used to develop assessments for EU directives. If a 95% target were to be used as a regional indicator of MSFD GES, Iceland and offshore...
Tipo: Text
Palavras-chave: ICON; Contaminants; European seas; Biological effects; Assessment.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00335/44583/44301.pdf

Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production
Provedor de dados: 5
Autores: Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text
Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf

Effets d'un mélange de pesticides sur les hémocytes d'huître creuse, Crassostrea gigas.
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Geret, Florence; Haure, Joel; Martin, Jean-louis; Burgeot, Thierry; Thomas-guyon, Hélène; Renault, Tristan.
Shellfish industry is often realised in estuarine areas, subjected to pollutions due to agricultural activities. The harmful effects of pesticides on animals inhabiting these estuarine zones are poorly documented. Bivalve molluscs represent an interesting model because they are sedentary and they filter water intensively. Effects of pesticides on haemocytes functions were tested in Pacific oyster, Crassostrea gigas and analysed by flow cytometry. A combination of pesticide at environmental concentrations was tested in vivo. Animals used corresponded to two genetically selected populations produced in hatchery.
Tipo: Text
Palavras-chave: Haemocytes; Flow cytometry; Pesticides; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-924.pdf

Risk assessment of herbicides and booster biocides along estuarine continuums in the Bay of Vilaine area (Brittany, France)
Provedor de dados: 5
Autores: Caquet, Th.; Roucaute, M.; Mazzella, N.; Delmas, F.; Madigou, C.; Farcy, E.; Burgeot, Thierry; Allenou, Jean-pierre; Gabellec, Raoul.
A 2-year study was implemented to characterize the contamination of estuarine continuums in the Bay of Vilaine area (NW Atlantic Coast, Southern Brittany, France) by 30 pesticide and biocide active substances and metabolites. Among these, 11 triazines (ametryn, atrazine, desethylatrazine, desethylterbuthylazine, desisopropyl atrazine, Irgarol 1051, prometryn, propazine, simazine, terbuthylazine, and terbutryn), 10 phenylureas (chlortoluron, diuron, 1-(3,4-dichlorophenyl)-3-methylurea, fenuron, isoproturon, 1-(4-isopropylphenyl)-3-methylurea, 1-(4-isopropylphenyl)-urea, linuron, metoxuron, and monuron), and 4 chloroacetanilides (acetochlor, alachlor, metolachlor, and metazachlor) were detected at least once. The objectives were to assess the corresponding...
Tipo: Text
Palavras-chave: Estuary; Herbicides; Booster biocides; Species sensitivity distribution; Risk quotient; AtlanticCoast.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23571/22554.pdf

The dragonet Callionymus lyra, a target species used for evaluation of biological effects of chemical contaminants on French coasts
Provedor de dados: 5
Autores: Burgeot, Thierry; Bocquene, Gilles; Truquet, Philippe; Le Dean, Loic; Poulard, Jean-charles; Dorel, Didier; Souplet, Arnauld; Galgani, Francois.
An oceanographic cruise in 1991 along the English Channel and Atlantic coasts of France indicated the value of dragonet Callionymus lyra Linnaeus, 1758 as a target species for monitoring biological effects. Ethoxyresorufin-O-deethylase measurements in this species provided a first assessment of the effects of hydrocarbons (PCB, PAH, dioxins) on the marine environment. The specific behavior of dragonet was defined by biochemical characterization. Biological data were obtained during 3 additional oceanographic cruises to study changes in the abundance and distribution of the species in the English Channel and the Bay of Biscay. This first assessment, carried out in the context of the National Observation Network, indicated that pollutants do not have...
Tipo: Text
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00091/20243/17895.pdf

Editorial: New Challenges in Marine Pollution Monitoring
Provedor de dados: 5
Autores: Bellas, Juan; Hylland, Ketil; Burgeot, Thierry.
Tipo: Text
Palavras-chave: Marine pollution; Ecotoxicology; Biomonitoring; Water quality; Bioaccumulation.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71775/70269.pdf

Pesticides and Ostreid Herpesvirus 1 Infection in the Pacific Oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Moreau, Pierrick; Faury, Nicole; Burgeot, Thierry; Renault, Tristan.
Since 2008, mass mortality outbreaks have been reported in all French regions producing Pacific oysters, and in several Member States of the European Union. These mass mortality events of Pacific oysters are related to OsHV-1 infection. They occur during spring and summer periods leaving suspect the quality of the marine environment and the role of seasonal use of pesticides associated with the arrival of freshwater in oyster rearing areas. Pesticides have been also detected in French coastal waters, especially in areas of oyster production. Using PMA real-time PCR we showed that a mixture of 14 pesticides has no effect on the integrity of virus capsids from viral suspension in the conditions tested. A contact of oysters with this pesticide mixture was...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00273/38405/36730.pdf

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts
Provedor de dados: 5
Autores: Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry.
Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation...
Tipo: Text
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf

Essai de décontamination à l'échelle professionnelle de moules rendues toxiques par le phytoplancton
Provedor de dados: 5
Autores: Masson, Daniel; Ratiskol, Gilles; Burgeot, Thierry; Margat, Sylvie.
Les secteurs conchylicoles atteints par les efflorescences phytoplanctoniques toxiques sont fermés par l'Administration dès que le taux de contamination devient dangereux pour la consommation humaine. Cette fermeture peut durer plusieurs mois empêchant les conchyliculteurs de commercialiser leurs produits, ce qui a des conséquences économiques parfois graves. Il n'existe jusqu'à présent aucune possibilité technique de décontaminer ces coquillages en grande quantité et dans des délais économiquement viables. L'étude entreprise visait à mettre au point une technique permettant aux professionnels de décontaminer des coquillages rapidement (quinze jours au moins) en milieu contrôlé (bassins). L'hypothèse testée était que des coquillages placés dans un milieu...
Tipo: Text
Palavras-chave: Coquillages; Décontamination; Toxicité; Phytoplancton.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00134/24499/22527.pdf

La surveillance de la qualité du milieu marin : l'expérience du Réseau National d'Observation (RNO) et le développement de biomarqueurs
Provedor de dados: 5
Autores: Amiard-triquet, Claude; Burgeot, Thierry; Claisse, Didier.
Monitoring of marine environment quality can be based on chemical analysis of contaminants in the major components of the environment: seawater, sediment, biota. The respective interest of these different matrices is discussed and the fundamentals for the use of bivalves in the international Mussel Watch are presented. The objectives of the French RNO are 1) to assess the levels and trends of contaminants; 2) to assess the biological effects of contaminants. Analysis of the chemicals in biota is an indispensable complement to the biological approach, allowing their bioavailable fraction to be quantified. However, only a fraction of xenobiotics incorporated in living beings is biologically active and can exert a noxious effect on the biota. In addition,...
Tipo: Text
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00017/12848/9793.pdf

Monitoring of pollutant biochemical effects on marine organisms of the french coasts
Provedor de dados: 5
Autores: Galgani, Francois; Bocquene, Gilles; Truquet, Philippe; Burgeot, Thierry; Chiffoleau, Jean-francois; Claisse, Didier.
The establishment of a network to assess pollutant effects is a current concern of international bodies such as the North Sea Task Force, the International Council for the Exploration of the Sea and the Intergovernmental Oceanographic Commission. With respect to the French coasts, three major considerations have been defined : the choice of monitoring areas ; the determination of a target species according to different criteria such as wide distribution, limited migration and contact with pollutant-rich sediments; and the selection of suitable parameters. This paper concerns the strategy adopted for the French coast. Ethoxyresorufin-O-deethylase, a specific cytochrome P450-dependent monooxygenase, measured by a simple microplate assay, was selected as an...
Tipo: Text
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00100/21169/18787.pdf

Etude l'écotoxicité des sédiments dragues - Documents de synthèse et annexes - Projet du groupe GEODE
Provedor de dados: 5
Autores: Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne.
La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude.
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf

Campagne dédiée à l'évaluation des effets biologiques induits pas la contamination chimique en Baies de Loire et Vilaine. SELILOIRE 2017
Provedor de dados: 5
Autores: Mauffret, Aourell; Wessel, Nathalie; Akcha, Farida; Munschy, Catherine; Chouvelon, Tiphaine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Aminot, Yann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Menard, Dominique; Burgeot, Thierry.
Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Des soles ont été prélevées en août et septembre 2017 et des moules en février 2018, dans le cadre du suivi ROCCH MV. Les biomarqueurs suivis chez ces organismes couvrent plusieurs mécanismes d’actions toxiques (reprotoxicité, génotoxicité, neurotoxicité) et incluent des mesures de la santé générale de l'organisme. Les contaminants suivis sont des éléments traces métalliques et des contaminants organiques...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00620/73215/72410.pdf

Study of genetic damage in the japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of dna damage
Provedor de dados: 5
Autores: Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah.
Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland.  The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg...
Tipo: Text
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf

Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects
Provedor de dados: 5
Autores: Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All...
Tipo: Text
Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf

DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas
Provedor de dados: 5
Autores: Hylland, Ketil; Skei, Bjorn Borge; Brunborg, Gunnar; Lang, Thomas; Gubbins, Matthew J.; Le Goff, Jeremie; Burgeot, Thierry.
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and...
Tipo: Text
Palavras-chave: ICON; Comet; DNA adducts; Genotoxicity; Haddock; Dab; European seas.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00309/42064/44971.pdf

Conditions d'apparition de densités accrues de dinoflagellés Dinophysis dans le pertuis d'Antioche et les eaux adjacentes. Campagnes Dinopertuis 89
Provedor de dados: 5
Autores: Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean.
Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All...
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf

Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis
Provedor de dados: 5
Autores: Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf

L'éthoxyrésorufine-o-déethylase, les adduits à l'ADN et les mocronuclei dans les organismes marins. Application à la surveillance des effets biologiques sur les côtes françaises
Provedor de dados: 5
Autores: Burgeot, Thierry.
Three biomarkers [ethoxyresorufm-o-deethylase (EROD), DNA adducts and micronuclei (MN) were studied for biomonitoring applications along French coasts. EROD was developed at an experirnental stage in two pilot sites, Seine Bay and Fos sur Mer, using the dab (Limanda limanda), dragon et (Callionymus lyra), red mullet (Mullus barbatus) and comber (Serranus hepatus) as target species. Higbest EROD activity was observed in the subestuarial part of Seine Bay and at the stations closest to sources of pollution from Cortiou and Fos sur Mer. The suitable number of fish for detection of a significant difference between two EROD activities was deterrnined as a function of sampling variance and a preselected statistical power. A preliminary study on hepatocytes in a...
Tipo: Text
Palavras-chave: Ethoxyrésorufine-o-déethylase; Adduits à l'ADN; Micronuclei; Poisson; Moule; Huître; Biosurveillance; Ethoryresorufin-o-deethylase; DNA adducts; Micronuclei; Fish; Mussel; Oyster; Biomonitoring.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00443/55462/56984.pdf

Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf

Descriptif des stations d'épuration ayant un rejet à la mer. Proposition d'un système de contrôle définissant la qualité du milieu marin
Provedor de dados: 5
Autores: Burgeot, Thierry; Fillon, Alain.
La surveillance et le suivi de la qualité du milieu est une des missions essentielles confiées au Laboratoire CSRU. Au Laboratoire de La Rochelle, le problème de l'assainissement et en particulier des rejets en mer, dans les zones conchylicoles des effluents des stations d'épuration fait l'objet, depuis plusieurs années, d'un contrôle régulier. Chaque contrôleur assure cette tâche par des prélèvements, effectués dans les différentes stations de leur circonscription.
Tipo: Text
Palavras-chave: Station épuration; Rejet; Milieu marin; Effluent; Système contrôle.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/00149/26029/24130.pdf

Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization
Provedor de dados: 5
Autores: Barranger, Audrey; Benabdelmouna, Abdellah; Degremont, Lionel; Burgeot, Thierry; Akcha, Farida.
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in...
Tipo: Text
Palavras-chave: Aneuploidy; FISH; Diuron; Crassostrea gigas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00238/34911/33216.pdf

A novel "Integrated Biomarker Response" calculation based on reference deviation concept
Provedor de dados: 5
Autores: Sanchez, Wilfried; Burgeot, Thierry; Porcher, Jean-marc.
Multi-biomarker approaches are used to assess ecosystem health and identify impacts of environmental stress on organisms. However, exploration of large datasets by environmental managers represents a major challenge for regulatory application of this tool. Several integrative tools were developed to summarize biomarker responses. The aim of the present paper is to update calculation of the "Integrated Biological Response" (IBR) described by Beliaeff and Burgeot (Environ Toxicol Chem 21:1316-1322, 2002) to avoid weaknesses of this integrative tool. In the present paper, a novel index named "Integrated Biological Responses version 2" based on the reference deviation concept is presented. It allows a clear discrimination of sampling sites as for the IBR, but...
Tipo: Text
Palavras-chave: Biomarkers; Data analysis; Graphic tools; Three-spined stickleback.
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00139/25069/23191.pdf

Défi MOREST - Mortalités etivales de l'huître creuse Crassostrea gigas - Caractérisation des facteurs de risques associés aux mortalités estivales - Synthèse du thème 4B - Caractérisation phénotypique des souches R Résistantes et S Susceptibles à la mortalité estivale. (La Rochelle 14-15 mars 2006)
Provedor de dados: 5
Autores: Huvet, Arnaud; Royer, J.; Moal, J.; Burgeot, Thierry; Lapègue, Sylvie; Boulo, Viviane; Nicolas, Jean-louis; Lambert, C.
Les résultats du programme MOREST démontrent l'existence d'une composante génétique à la survie estivale du naissain et nous permettent de disposer aujourd'hui d'huTtres « résistantes» (R) et « susceptibles» (S) aux mortalités estivales (Dégremont et aL, 2002, 2005). La forte héritabilité estimée face à un tel système multifactoriel permet de suspecter que le caractère sélectionné puisse être gouverné par un ou un trés petit nombre de gènes sur une région chromosomique donnée. D'après le modèle d'interaction suspecté entre l'environnement, l'huître et des pathogènes opportunistes, une hiérarchie des facteurs, conduisant aux mortalités, permet de montrer qu'aucun d'entre eux ne peut provoquer à lui seul les mortalités mais que leur combinaison est...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00306/41706/40917.pdf

Evaluation du descripteur 8 « Contaminants dans le milieu » en France Métropolitaine.
Provedor de dados: 5
Autores: Mauffret, Aourell; Chiffoleau, Jean-francois; Burgeot, Thierry; Wessel, Nathalie; Brun, Melanie.
Good Environmental Status (GES) for descriptor 8 of MSFD is reached when concentrations of contaminants are at levels not giving rise to pollution effects. According to the decision 2017/848/CE, descriptor 8 is defined by four criteria: concentration in the relevant matrices (sediment, biota) (D8C1), effects of chronic contamination to the marine organisms (D8C2), duration and spatial extent of acute pollution events (D8C3), effects of acute contamination to the marine organisms (D8C4). The GES for descriptor 8 is assessed at the level of four marine subregions (SRM), including: Channel – North Sea (MMN), Celtics Seas (MC), Bay of Biscay (GdG) and the Occidental Mediterranean Sea (MO). In order to achieve GES, each indicator of descriptor 8 should respect...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57294/59374.pdf

Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp, in Seine Bay: A key step towards applying biological effects to monitoring
Provedor de dados: 5
Autores: Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil.
The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons...
Tipo: Text
Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf

Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed
Provedor de dados: 5
Autores: Akcha, Farida; Burgeot, Thierry; Budzinski, H; Pfohla Leszkowicz, A; Narbonne, J.
With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg(-1) dry wt mussel d(-1)) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was...
Tipo: Text
Palavras-chave: DNA repair; 8 oxodGuo; DNA adducts; Mussel; BaP.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-813.pdf

Effets de la contamination chimique : des organismes en danger ?
Provedor de dados: 5
Autores: Poisson, Emeline; Fisson, Cédric; Amiard-triquet, Claude; Burgeot, Thierry; Couteau, Jérôme; Dur, Gael; Durand, Fabrice; Forget-leray, Joelle; Letendre, Julie; Souissi, Sami; Xuereb, Benoit.
Le présent fascicule a pour objectif d’apporter les éléments de réponse disponibles pour l’estuaire de la Seine sur ce questionnement. Dans la première partie écotoxicologie. Les effets de la contamination chimique à différents niveaux d’organisation biologique, sur les organismes présents dans l’estuaire de la Seine, seront présentés dans la deuxième partie puis synthétisés dans notion d’analyse du risque environnemental.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00319/43019/42534.pdf

Replicated anthropogenic hybridisations reveal parallel patterns of admixture in marine mussels
Provedor de dados: 5
Autores: Simon, Alexis; Arbiol, Christine; Nielsen, Einar Eg; Couteau, Jérôme; Sussarellu, Rossana; Burgeot, Thierry; Bernard, Ismaël; Coolen, Joop W. P.; Lamy, Jean-baptiste; Robert, Stephane; Skazina, Maria; Strelkov, Petr; Queiroga, Henrique; Cancio, Ibon; Welch, John J.; Viard, Frédérique; Bierne, Nicolas.
Human‐mediated transport creates secondary contacts between genetically differentiated lineages, bringing new opportunities for gene exchange. When similar introductions occur in different places, they provide informally replicated experiments for studying hybridisation. We here examined 4,279 Mytilus mussels, sampled in Europe and genotyped with 77 ancestry‐informative markers. We identified a type of introduced mussels, called “dock mussels,” associated with port habitats and displaying a particular genetic signal of admixture between M. edulis and the Mediterranean lineage of M. galloprovincialis. These mussels exhibit similarities in their ancestry compositions, regardless of the local native genetic backgrounds and the distance separating colonised...
Tipo: Text
Palavras-chave: Admixture; Bentho-pelagic species; Biological introductions; Clines; Ports; Secondary contact.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70600/68791.pdf

How can we quantify impacts of contaminants in marine ecosystems? The ICON project
Provedor de dados: 5
Autores: Hylland, Ketil; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Robinson, Craig D.; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Matthew J.
An international workshop on marine integrated contaminant monitoring (ICON) was organised to test a framework on integrated environmental assessment and simultaneously assess the status of selected European marine areas. Biota and sediment were sampled in selected estuarine, inshore and offshore locations encompassing marine habitats from Iceland to the Spanish Mediterranean. The outcome of the ICON project is reported in this special issue as method-oriented papers addressing chemical analyses, PAH metabolites, oxidative stress, biotransformation, lysosomal membrane stability, genotoxicity, disease in fish, and sediment assessment, as well as papers assessing specific areas. This paper provides a background and introduction to the ICON project, by...
Tipo: Text
Palavras-chave: ICON; Contaminants; North sea; Mediterranean sea; Biological effects; Biomarkers; Monitoring.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00297/40782/44970.pdf

Tributyltin: A Bottom–Up Regulator of the Crangon crangon Population?
Provedor de dados: 5
Autores: Parmentier, Koen F. V.; Verhaegen, Yves; De Witte, Bavo P.; Hoffman, Stefan; Delbare, Daan H. R.; Roose, Patrick M.; Hylland, Ketil D. E.; Burgeot, Thierry; Smagghe, Guy J.; Cooreman, Kris.
The restrictions and the concerted action of the global ban on the use and presence of tributyltin (TBT) in marine applications to protect ecosystems in the marine environment in 2008 was mainly based on the economic impact on shellfish industries and the dramatic extinction of local mollusk populations in the past. In contrast to the vast datasets on effects on mollusks, the knowledge on impacts on species from other taxa remained in the uncertain until almost two decades ago. The assumption on a long-term TBT-mediated pernicious metabolic bottom–up regulation of the crustacean Crangon crangon population was provoked by the outcome of an EU-project ‘Sources, Consumer Exposure and Risks of Organotin Contamination in Seafood.’ This study reported high TBT...
Tipo: Text
Palavras-chave: Tributyltin; Crangon crangon; Endocrine disruption; Ecosystem impact; Ecdysteroids; Ecdysteroid receptor.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00585/69727/67617.pdf

Environmental stress in the pacific oyster Crassostrea gigas during gametogenesis : role of herbicides?
Provedor de dados: 5
Autores: Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F.
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines.  On the other hand, a significant increase in vitellin production was...
Tipo: Text
Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf

In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families.
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf

Surveillance des efflorescences phytoplanctoniques en Charente-Maritime en 1988
Provedor de dados: 5
Autores: Burgeot, Thierry; Fillon, Alain; Ratiskol, Gilles; Vayne, Jean-jacques.
La synthèse des informations collectées en 1988 en Charente Maritime, en matière de développement microalgaux a pour but de mettre en évidence, à partir des données recueillies au cours d'une année, des tendances et niveaux de contaminations phytoplanctoniques, ainsi qu'une certaine identité du secteur surveillé. L'année 1988 est marquée par une efflorescence monospécifique de Gymnodinium sp. en période estivale et par ['apparition du Dinophysis à des concentrations supérieures au seuil toxique sans qu'aucune toxicité ne soit décelée. Le réseau de suivi confirme, à travers la bonne représentativité des stations de prélèvement, la spécificité des pertuis charentais.
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00119/23025/20856.pdf

Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast
Provedor de dados: 5
Autores: Cachot, Jerome; Cherel, Yan; Larcher, Thibaut; Pfohl-leszkowicz, Annie; Laroche, Jean; Quiniou, Louis; Morin, Jocelyne; Schmitz, Julien; Burgeot, Thierry; Pottier, Didier.
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of...
Tipo: Text
Palavras-chave: Epidemiological study; European flounder; Seine estuary; Liver histopathology; Necrosis; FCA; Tumors; Inflammations; Parasites; DNA adducts; Pollution.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23576/21498.pdf

Suivi des efflorescences phytoplanctoniques en Charente-Maritime en 1988
Provedor de dados: 5
Autores: Burgeot, Thierry; Fillon, Alain; Ratiskol, Gilles; Vayne, Jean-jacques.
La synthèse des informations collectées en 1988 en Charente Maritime, en matière de développement microalgaux a pour but de mettre en évidence, à partir des données recueillies au cours d'une année, des tendances et niveaux de contaminations phytoplanctoniques, ainsi qu'une certaine identité du secteur surveillé. L'année 1988 est marquée par une efflorescence monospécifique de Gymnodinium sp. en période estivale et par l'apparition du Dinophysis à des concentrations supérieures au seuil toxique sans qu'aucne toxicité ne soit décelée. Le réseau de suivi confirme, à travers la bonne représentativité des stations de prélèvement, la spécificité des pertuis charentais
Tipo: Text
Palavras-chave: Microalgues; Pertuis charentais; Efflorescences monospécifiques.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00443/55496/57039.pdf

Monitoring of trace metal contamination in the Souss estuary (South Morocco) using the clams Cerastoderma edule and Scrobicularia plana
Provedor de dados: 5
Autores: Anajjar, E; Chiffoleau, Jean-francois; Bergayou, H; Moukrim, A; Burgeot, Thierry; Cheggour, M.
Concentrations of seven metals (Cd, Cu, Cr, Pb, Ni, Zn, Hg) were monitored monthly during 2 years in two bivalves, Cerastoderma edule and Scrobicularia plana collected from the Souss estuary (South Morocco) before and after the commissioning of a sewage treatment plant in November 2002. According to trace metals, changes in concentrations were observed mainly in S. plana, characterised by a decrease of Cu, Hg and Pb when Cd was clearly increasing. Conversely, no clear changes were observed in C. edule during the same periods. This study suggests the use of S. plana in the monitoring of trace metal contamination of such coastal marine environments.
Tipo: Text
Palavras-chave: Monitoring; Waste water; Bivalves; Metals; South estuary.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4515.pdf

Spatial variations in biomarkers of Mytilus edulis mussels at four polluted regions spanning the Northern Hemisphere
Provedor de dados: 5
Autores: Gagne, F; Burgeot, Thierry; Hellou, J; St Jean, S; Farcy, E; Blaise, C.
Economic and social developments have taken place at the expense of the health of the environment, both locally and on a global scale. In an attempt to better understand the large-scale effects of pollution and other stressors like climate change on the health status of Mytilus edulis, mussels were collected during the first two weeks of June 2005 at three sites (one pristine and two affected by pollution) located in each of the regions of the Canadian West Coast, the St. Lawrence estuary, the Atlantic East Coast and the northwestern coast of France, covering a total distance of some I 1000 km. The mussels were analyzed for morphologic integrity (condition factor), gametogenic activity (gonado-somatic and gonad maturation index, vitellogenin(Vtg)-like...
Tipo: Text
Palavras-chave: Health status; Mytilus edulis; Global changes; Pollution; Biomarkers.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4342.pdf

Effects of increasing temperatures on biomarker responses and accumulation of hazardous substances in rope mussels (Mytilus galloprovincialis) from Bizerte lagoon
Provedor de dados: 5
Autores: Kamel, Naouel; Burgeot, Thierry; Banni, Mohamed; Chalghaf, Mohamed; Devin, Simon; Minier, Christophe; Boussetta, Hamadi.
This study examined the influence of increasing temperatures in spring and summer on biochemical biomarkers in Mytilus galloprovincialis mussels sampled from Bizerte lagoon (northern Tunisia). Spatial and seasonal variations in a battery of seven biomarkers were analyzed in relation to environmental parameters (temperature, salinity and pH), physiological status (condition and gonad indexes), stress on stress (SoS) and chemical contaminant levels (heavy metals, PAHs and PCBs) in digestive glands. Integrated Biological Response (IBR) was calculated using seven biomarkers (AChE, BPH, MXR, GST, CAT, MDA and MT). Seasonal variations in biological response were determined during a critical period between spring and summer at two sites, where chemical...
Tipo: Text
Palavras-chave: Biomarkers; Mussels; Lagoon biomonitoring; Integrated biomarker response.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00171/28224/26471.pdf

Changes in the sediment trace metal contamination after the commissioning of a municipal wastewater treatment plant in the Souss estuary (South Morocco)
Provedor de dados: 5
Autores: Moukrim, A; Chiffoleau, Jean-francois; Cheggour, M; Burgeot, Thierry.
Monthly samples of sediment were collected from the Souss estuary, to determine the changes which have occurred in the concentrations of ten metals between 2001 when the low estuary was receiving the wastewaters from Agadir city and 2003 after stopping the sewage dumping. The annual contents of Cu, Fe, Zn (in 2001) and those of Cr, Cu, Fe, Mn, Ni, Pb, and Zn (in 2003) showed significant correlations with those of Al, indicating that fluctuations of their concentrations were only due to those of the aluminosilicate fraction of the clayey sediment. After normalization of these metallic concentrations in relation to those of Al, the contents of the different metals showed insignificant correlations between both years, except for Zn which significantly dropped...
Tipo: Text
Palavras-chave: Souss estuary; Wastewater; Sediment; Metal contamination.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5923.pdf

PAH metabolites in fish bile: from the Seine Estuary to Iceland
Provedor de dados: 5
Autores: Kammann, U.; Akcha, Farida; Budzinski, H.; Burgeot, Thierry; Gubbins, M. J.; Lang, T.; Le Menach, K.; Vethaak, A. D.; Hylland, K.
Polycyclic aromatic hydrocarbons (PAH) are environmental contaminants that pose significant risk to health of fish. The International Workshop on Integrated Assessment of Contaminant Impacts on the North Sea (ICON) provided the framework to investigate biomarker responses as well as contaminant concentrations side by side in marine ecosystems. Concentrations of the main PAH metabolites 1-hydroxypyrene, 1-hydroxyphenanthren and 3-hydroxybenzo(a)pyrene were determined in bile by HPLC with fluorescence detection. Fish species under investigation were dab (Limanda limanda), flounder (Platichthys flesus) and haddock (Melanogrammus aeglefinus). A contamination gradient was demonstrated from the low contaminated waters of Iceland and off-shore regions of the...
Tipo: Text
Palavras-chave: PAH metabolite; Dab; Flounder; Haddock; Marine monitoring; Atlantic; North Sea.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00317/42861/42288.pdf

Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such   contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly  known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf

Effects of water accommodated fractions of crude oils and diesel on a suite of biomarkers in Atlantic cod (Gadus morhua)
Provedor de dados: 5
Autores: Holth, T. F.; Eidsvoll, D. P.; Farmen, E.; Sanders, M. B.; Martinez-gomez, C.; Budzinski, H.; Burgeot, Thierry; Guilhermino, L.; Hylland, K.
The aim of this study was to characterise concentration- and time-dependent responses in juvenile Atlantic cod (Gadus morhua) following exposure for one and three weeks to the water-soluble fraction (WAF) of three weathered oils: Arabian Light crude oil (ALC), North Sea crude oil (NSC) and ship-diesel. The sum of polycyclic aromatic hydrocarbons (PAH) in water was highest after one week of exposure and within environmentally relevant concentrations. PAH metabolites in bile confirmed exposure to and uptake of PAHs. Hepatic cytochrome P450 1A (CYP1A) gene expression (here and later the phrase gene expression is used synonymously to gene transcription, although it is acknowledged that gene expression is also regulated by, e.g., translation and protein...
Tipo: Text
Palavras-chave: Atlantic cod; Biomarkers; Weathered crude oil; Ship-diesel; Water accommodated fraction; Time-course.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30131/28593.pdf

Integrated indicator framework and methodology for monitoring and assessment of hazardous substances and their effects in the marine environment
Provedor de dados: 5
Autores: Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil.
Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for...
Tipo: Text
Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf

Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf

Contaminant exposure and ecotoxicological impacts in estuaries
Provedor de dados: 5
Autores: Burgeot, Thierry; Gagne, Francois.
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23568/22482.pdf

Mortalité de Crassostrea gigas dans le bassin de Marennes Oléron. Etude physico chimique du sédiment. Etude du modèle de mortalité "plat-table" de C. gigas dans le bassin de Marennes Oléron. Etude "Dynamo" du projet MOREST
Provedor de dados: 5
Autores: Soletchnik, Patrick; Bouchet, Vincent; Malestroit, Pascal; Seugnet, Jean-luc; Blouin, Frederic; Radford-knoery, Joël; Burgeot, Thierry; Sauriau, Pierre-guy.
In the scope of the study of the "on-bottom/off-bottom" mortality model in the south of the Marennes Oléron Bay, the result of the 2003 multidisciplinary study (Dynamor) appears to be the hypothesis of an acute stress, "ultimate" trigger of the mortality episodes in June. In 2004 we aim to look for the nature of this stress through the physico-chemical analysis of the sediment (Eh, pH, sulphides and ammoniacal nitrogen), the organic matter and chlorophyll load on the surface of the sediment between April and July. Herbicides are also measured in the water column, south of the bay (Seudre), during the same period. A "precocious" (around the first week of June) and "weak" (lower mortality rate on this working site since 1997) mortality peak happens when the...
Tipo: Text
Palavras-chave: Bassin Marennes Oléron; Phytobenthos; Mortalité; Crassostrea gigas; Foraminiferes benthiques; Répartition verticale; Sédiment; Huître; Echantillonnage.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/rapport-3383.pdf

Assessment of contaminant concentrations in sediments, fish and mussels sampled from the North Atlantic and European regional seas within the ICON project
Provedor de dados: 5
Autores: Robinson, Craig D.; Webster, Lynda; Martinez-gomez, Concepcion; Burgeot, Thierry; Gubbins, Matthew J.; Thain, John E.; Vethaak, A. Dick; Mcintosh, Alistair D.; Hylland, Ketil.
Understanding the status of contaminants in the marine environment is a requirement of European Union Directives and the Regional Seas Conventions, so that measures to reduce pollution can be identified and their efficacy assessed. The international ICON workshop (Hylland et al., in this issue) was developed in order to test an integrated approach to assessing both contaminant concentrations and their effects. This paper describes and assesses the concentrations of trace metals, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in sediments, mussels, and fish collected from estuarine, coastal and offshore waters from Iceland to the Mediterranean Sea. For organic contaminants, concentrations progressively increased from Iceland, to the...
Tipo: Text
Palavras-chave: Integrated assessment; ICES; North Atlantic; North Sea; Baltic Sea; Mediterranean Sea; Polycyclic aromatic hydrocarbons (PAHs); Polychlorinated biphenyls (PCBs); Trace metals; Environmental monitoring.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00324/43520/43051.pdf

Multigenerational exposure of the microalga Tetraselmis suecica to diuron leads to spontaneous long-term strain adaptation.
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Jerome, Marc; Rouxel, Julien; Khelifi, Celia; Rince, Maeva; Burgeot, Thierry.
To investigate the ability of microalgae to develop stable, long-term resistance to herbicides, the marine microalga Tetraselmis suecica was exposed to the herbicide diuron (5 µg/L) for a 43-generation exposure period followed by a 12-generation depuration phase. During the first 25 generations, diuron-exposed cultures showed doubling times ranging from 1.95 to 2.6 days, which was 2 to 2.5-fold longer than control cultures. Between generations 25 and 38, during diuron exposure, two out of the three exposed cultures exhibited a spontaneous drop in doubling time. These results provided evidence of culture adaptation to diuron. To assess persistence of the diuron adaptation observed on growth performance, one of the adapted cultures (D3) was maintained for 12...
Tipo: Text
Palavras-chave: Microalgae; Diuron; Multigenerational; Adaptation; Flow cytometry.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00146/25718/23796.pdf

Bioindicators of pollutant exposure in the northwestern Mediterranean sea
Provedor de dados: 5
Autores: Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois.
Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),...
Tipo: Text
Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf

Expertise de l'Ifremer sur la contamination significative historique en milieu marin, en particulier par des métaux toxiques tels que le mercure et l'arsenic par la société Alteo
Provedor de dados: 5
Autores: Dreves, Luc; Amouroux, Isabelle; Gonzalez, Jean-louis; Knoery, Joel; Andral, Bruno; Chiffoleau, Jean-francois; Brach-papa, Christophe; Claisse, Didier; Burgeot, Thierry.
Expertise réalisée dans le cadre de la saisine, datée du 10 octobre 2014, auprès du PDG de l'Ifremer de la Ministre de l’Écologie, du Développement Durable et de l’Énergie, sur la demande de poursuite de rejet d'effluents industriels liquides en Méditerranée
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00260/37099/35615.pdf

Caractérisation des facteurs de risques associés au mortalités estivales - Synthèse du thème 3 - Risque associé au stress environnemental - 2002-2005
Provedor de dados: 5
Autores: Burgeot, Thierry; Gagnaire, Beatrice; Renault, Tristan; Haure, Joel; Moraga, D.; David, E.; Boutet, L.; Sauriau, P.g.; Malet, Nathalie; Bouchet, V.; Lapegue, Sylvie; Bouilly, Karine; Le Moullac, Gilles; Arzul, Genevieve; Knoery, Joel; Quiniou, Francoise; Bacher, Cedric; Soletchnik, Patrick.
Les mortalités d'huîtres observées sur les côtes françaises apparaissent principalement à proximité du sédiment et pendant la gamétogenèse. Les interactions de facteurs environnementaux sur les écosystèmes conchylicoles comme la température, les apports des bassins versants et le compartiment sédimentaire illustrent un risque de mortalité lié à différentes sources de stress (Thème 1). Les variations de la qualité de la ressource trophique pendant l'effort de gamétogenèse favorise une baisse de l'énergie disponible (Thème 2) et contraint l'organisme fragilisé à fournir un effort d'adaptation (Fent 2004). L'apparition d'un stress pendant cette période sensible des huîtres pourrait constituer un facteur aggravant de mortalité. L'objectif de cette étude est...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00304/41538/40746.pdf

The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects
Provedor de dados: 5
Autores: Wessel, Nathalie; Menard, Dominique; Pichavant-rafini, K.; Ollivier, H.; Le Goff, Jean-michel; Burgeot, Thierry; Akcha, Farida.
The benzo[a]pyrene is a polycyclic aromatic hydrocarbon known to be genotoxic, mutagenic and carcinogenic in higher vertebrates. The aim of this study was to evaluate in vitro the enzymatic and genotoxic effects of BaP in a benthic fish species, Solea solea. Sole hepatocytes were exposed to BaP in order to measure the modulation of ethoxyresorufin-o-deethylase (EROD) activity and the DNA strand breaks induced by BaP metabolism. Exposures were performed in both culture flasks and microplate wells in order to check for the possible miniaturization of the exposure system. Moreover, sole liver microsomes were exposed to BaP in the presence of standard DNA in order to assess the potential formation of DNA adducts in sole. The results demonstrated the ability of...
Tipo: Text
Palavras-chave: Genotoxicity; Sole; BaP; DNA strand breaks; EROD; DNA adducts.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12968/9944.pdf

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf

Hydrocarbon pollution along Moroccan coasts and BPH activity in the mussel Perna perna
Provedor de dados: 5
Autores: Azdi, M.; Moukrim, A.; Burgeot, Thierry; Budzinski, H.; Chiffoleau, Jean-francois; Kaaya, A.; Zekhnini, A.; Narbonne, J. F.; Guarrigues, Ph.
Specimens of the mussel Perna perna were collected along Moroccan coasts to determine the concentrations of polycyclic aromatic hydrocarbons ( PAHs) bioaccumulated in the tissues, and to measure benzo[ a] pyrene hydroxylase (BPH) activity. Chemical analysis of PAHs show that the Mediterranean (Nador, Martil, Tanger) and central Atlantic coasts ( from Rabat to Jorf Lihoudi) are those most contaminated ( 351 245 ng.g(-1) dry weight in Tanger). The mussel contaminants were of mixed origin for most of the locations with non negligible inputs of petrogenic origin in many of them. Baseline levels of PAHs were between 6 and 55 ng.g(-1) dry weight. BPH activity showed significant correlation (r(s) = 0.64, P &lt; 0.05) with total PAH concentrations at the six most...
Tipo: Text
Palavras-chave: Mussel; Perna perna; Moroccan coasts; Polycyclic aromatic hydrocarbons; Benzoapyrene hydroxylase; Baseline level.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00000/10995/12929.pdf