Consulta: creatorFacets:"CARVALHO,LUIZ J.C.B."
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Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis
Provedor de dados: 93
Autores: CABRAL,GLAUCIA B.; CARVALHO,LUIZ J.C.B.
Two-dimensional gel electrophoresis analysis was performed on adventitious and storage root in cassava (Manihot esculenta Crantz). Adventitious root lacking swelling formation and swelled storage root were obtained from the accession WU104 grown in the green house of the Department of Biology in Washington University in St. Louis (MO-USA). Saline buffer-soluble proteins were extracted, separated in a high-resolution 2-D electrophoresis system, visualized with silver staining gel procedure, and digital image generate for further analysis. Quantitative and qualitative protein spots analysis was performed with a computer assisted image software system. Results revealed large variation in the complexity of the gel protein profile between the two root systems....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Manihot esculenta; Storage root; 2-D gel electrophoresis; Proteins.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100005