Consulta: creatorFacets:"Cachot, Jerome"
Registros recuperados: 17
Data/hora: 02/06/2024 14:22:20

Qualité des milieux naturels
Provedor de dados: 5
Autores: Legube, Bernard; Montigny, Frédéric; Bustamante, Paco; Bareille, Gilles; Baudrimont, Magalie; Cachot, Jerome; Guibaud, Gilles; Hechard, Yann; Labanowski, Jérôme; Lacoue-labarthe, Thomas; Mondamert, Leslie; Budzinski, Hélène; Prou, Jean; Gazeau, Alain; Hulin, Agnès; Bunales, Rafaël; Villenave, Eric; Chabbi, Abad; Atteia, Olivier; Bouchon, Didier; Denaix, Agnès; Chery, Philippe; Pellerin, Sylvain; Rumpel, Cornelia.
L’impact du changement climatique sur la qualité et la pollution des milieux est souvent négligé par rapport à d’autres impacts plus médiatisés (e.g. événements climatiques et hydrologiques extrêmes, santé, trait de côtes, disponibilité des ressources en eau…). Les eaux et l’air de Nouvelle-Aquitaine, et à moindre mesure les sols, déjà significativement pollués, subiront pourtant plusieurs détériorations significatives, qui sont présentées et discutées dans ce chapitre. Certains de ces effets sont déjà visibles et vont s’aggraver dans le contexte climatique prévu. Il s’agit principalement, pour l’eau douce superficielle, de l’augmentation de la température, de la diminution des débits des cours d’eau, de l’évolution des espèces aquatiques et du...
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55578/57190.pdf

Long-term disruption of growth, reproduction, and behavior after embryonic exposure of zebrafish to PAH-spiked sediment
Provedor de dados: 5
Autores: Vignet, Caroline; Devier, Marie-hélène; Le Menach, Karyn; Lyphout, Laura; Potier, Jeremy; Cachot, Jerome; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier.
A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g−1 which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of...
Tipo: Text
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Lifecycle; Anxiety-like behavior; Offspring.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29909/28497.pdf

P53 et cancer
Provedor de dados: 5
Autores: Cachot, Jerome.
A la fin des années 70 une protéine était coprécipitée avec l'antigène T du virus simien 40 dans des cellules transformées par ce virus (Lane &amp; Crawford, 1979, Linzer &amp; levine, 1979). Cette protéine a été baptisée p53 en raison de son poids moléculaire de 53 kDa déterminé sur gel SDS-polyacrylamide. Le gène p53 n'a été découvert que quelques années plus tard dans des cellules transformées de souris (Oren &amp; levine, 1983). Il fut tout d'abord assimilé à un oncogène (Jenkins &amp; Sturzbecher, 1984a) avant d'être classé, en 1989, parmi les gènes suppresseur de tumeur (Hinds et al., 1989, Finlay et al., 1989). Il suscite depuis un intérêt croissant de la part de la communauté scientifique internationale. Le nombre de publications scientifiques a...
Tipo: Text
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00132/24351/22350.pdf

Chemicals sorbed to environmental microplastics are toxic to early life stages of aquatic organisms
Provedor de dados: 5
Autores: Cormier, Bettie; Gambardella, Chiara; Tato, Tania; Perdriat, Quentin; Costa, Elisa; Veclin, Cloé; Le Bihanic, Florane; Grassl, Bruno; Dubocq, Florian; Kärrman, Anna; Van Arkel, Kim; Lemoine, Soazig; Lagarde, Fabienne; Morin, Benedicte; Garaventa, Francesca; Faimali, Marco; Cousin, Xavier; Bégout, Marie-laure; Beiras, Ricardo; Cachot, Jerome.
Microplastics are ubiquitous in aquatic ecosystems, but little information is currently available on the dangers and risks to living organisms. In order to assess the ecotoxicity of environmental microplastics (MPs), samples were collected from the beaches of two islands in the Guadeloupe archipelago, Petit-Bourg (PB) located on the main island of Guadeloupe and Marie-Galante (MG) on the second island of the archipelago. These samples have a similar polymer composition with mainly polyethylene (PE) and polypropylene (PP). However, these two samples are very dissimilar with regard to their contamination profile and their toxicity. MPs from MG contain more lead, cadmium and organochlorine compounds while those from PB have higher levels of copper, zinc and...
Tipo: Text
Palavras-chave: Environmental microplastics; Leachates; Early life stages; Aquatic organisms; Toxicity.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00661/77276/78711.pdf

Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary
Provedor de dados: 5
Autores: Cachot, Jerome; Bultelle, Florence; Drouot, Laurent; Galgani, Francois; Frebourg, Thierry; Leboulenger, François; Danger, Jean-michel.
Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp 18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like...
Tipo: Text
Palavras-chave: X chromosome; Brain; Ovary; Human; Teleostean; Platichthys flesus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-756.pdf

Developmental toxicity of PAH mixtures in fish early life stages. Part II: adverse effects in Japanese medaka
Provedor de dados: 5
Autores: Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome.
In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different...
Tipo: Text
Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf

Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay
Provedor de dados: 5
Autores: Le Bihanic, Florane; Perrichon, Prescilla; Landi, Laure; Clerandeau, Christelle; Le Menach, Karyn; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome.
Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial...
Tipo: Text
Palavras-chave: Artificial sediment; Spiked sediment; PAHs; Japanese medaka embryos; Embryotoxicity; Teratogenicity.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00187/29872/28479.pdf

Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development
Provedor de dados: 5
Autores: Le Bihanic, Florane; Clérandeau, Christelle; Cormier, Bettie; Crebassa, Jean-claude; Keiter, Steffen H.; Beiras, Ricardo; Morin, Bénédicte; Bégout, Marie-laure; Cousin, Xavier; Cachot, Jerome.
The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations,...
Tipo: Text
Palavras-chave: Marine medaka; Microplastics; Developmental toxicity; Benzo(a)pyrene; Perfluorooctanesulfonic acid; Benzophenone-3.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00619/73112/74844.pdf

Réponse des organismes marins aux polluants bases biologiques et moléculaires
Provedor de dados: 5
Autores: Vincent, Francoise; Cachot, Jerome; Galgani, Francois; Bocquene, Gilles.
Analyse moléculaire de la mutagénèse Pour prédire les effets à long tenne de l'exposition aux polluants, la recherche s'oriente sur le développement, la validation et l'application de biomarqueurs de mutagénèse. Les mutations sur les gènes associés au développement tumoral peuvent être détectés dans des stades précoces du cancer, c'est le cas des gènes de la famille Ras et du gène supprésseur de tumeur P53. Les travaux ont porté sur l'étude de l'activation du protooncogène Ras chez les poissons, pour savoir si elle pouvait être corrélée avec l'apparition de tumeurs et à la présence de contaminants cancérigènes. Les exons 1 et 2 du gène Ras de Callionymus lira et de Mullus barbutus ont été amplifiés, clonés et séquencés pour détecter des mutations liées à...
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00132/24307/22305.pdf

Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout
Provedor de dados: 5
Autores: Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome.
A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic...
Tipo: Text
Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf

Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp, in Seine Bay: A key step towards applying biological effects to monitoring
Provedor de dados: 5
Autores: Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil.
The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons...
Tipo: Text
Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf

Toxicity assessment of water-accommodated fractions from two different oils using a zebrafish (Danio rerio) embryo-larval bioassay with a multilevel approach
Provedor de dados: 5
Autores: Perrichon, Prescilla; Le Menach, Karyn; Akcha, Farida; Cachot, Jerome; Budzinski, Helene; Bustamante, Paco.
Petroleum compounds from chronic discharges and oil spills represent an important source of environmental pollution. To better understand the deleterious effects of these compounds, the toxicity of water-accommodated fractions (WAF) from two different oils (brut Arabian Light and Erika heavy fuel oils) were used in this study. Zebrafish embryos (Danio rerio) were exposed during 96 h at three WAF concentrations (1, 10 and 100% for Arabian Light and 10, 50 and 100% for Erika) in order to cover a wide range of polycyclic aromatic hydrocarbon (PAH) concentrations, representative of the levels found after environmental oil spills. Several endpoints were recorded at different levels of biological organization, including lethal endpoints, morphological...
Tipo: Text
Palavras-chave: Zebrafish embryos; Oil exposure; Teratogenicity; Swimming performance; Bradycardia; Detoxification pathway.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00342/45324/46430.pdf

Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast
Provedor de dados: 5
Autores: Cachot, Jerome; Cherel, Yan; Larcher, Thibaut; Pfohl-leszkowicz, Annie; Laroche, Jean; Quiniou, Louis; Morin, Jocelyne; Schmitz, Julien; Burgeot, Thierry; Pottier, Didier.
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of...
Tipo: Text
Palavras-chave: Epidemiological study; European flounder; Seine estuary; Liver histopathology; Necrosis; FCA; Tumors; Inflammations; Parasites; DNA adducts; Pollution.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23576/21498.pdf

Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae
Provedor de dados: 5
Autores: Le Bihanic, Florane; Sommard, Vivien; De Lansalut, Perrine; Pichon, Anaïk; Grasset, Julie; Berrada, Saadia; Budzinski, Helene; Cousin, Xavier; Morin, Benedicte; Cachot, Jerome.
Benz[a]anthracene (BaA) is a ubiquitous polycyclic aromatic hydrocarbon found in numerous aquatic ecosystems. However, ecotoxicological data in aquatic organisms are scarce. To remedy this lack of data, Japanese medaka (Oryzias latipes) embryos were exposed to BaA and toxic effects were investigated at multiple toxicological endpoints. Japanese medaka embryos were incubated onto BaA-spiked artificial sediment for 9 days at low or moderate environmental concentrations ranging from 0.9 to 12 µg g−1 dw. BaA-exposed embryos exhibited significant tachycardia. BaA exposure was also shown to increase CYP1A activity in the hepato-biliary tissue as well as craniofacial deformities and DNA damage in pro-larvae. The photomotor response of BaA-exposed larvae was...
Tipo: Text
Palavras-chave: Benz[a]anthracene; Spiked sediment; Teratogenicity; DNA damage; Swimming behavior; In vivo EROD activity.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00244/35513/34729.pdf

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays
Provedor de dados: 5
Autores: Perrichon, Prescilla; Le Bihanic, Florane; Bustamante, Paco; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome; Cousin, Xavier.
Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals...
Tipo: Text
Palavras-chave: Fish embryo-larval assay; PAHs; Artificial sediment; EROD; Behavioral performance; Teratogenicity.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00242/35323/37905.pdf

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)
Provedor de dados: 5
Autores: Cormier, Bettie; Batel, Anika; Cachot, Jerome; Begout, Marie-laure; Braunbeck, Thomas; Cousin, Xavier; Keiter, Steffen H.
As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed...
Tipo: Text
Palavras-chave: Fish embryotoxicity test (FET); Swimming behavior; EROD; Cyp1a; Perfluorooctane sulfonate; Benzo[a]pyrene; Oxybenzone.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00514/62545/66896.pdf

PAHs and fish-exposure monitoring and adverse effects-from molecular to individual level
Provedor de dados: 5
Autores: Cousin, Xavier; Cachot, Jerome.
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00242/35321/33954.pdf