Consulta: creatorFacets:"Cambon-bonavita, Marie-anne"
Registros recuperados: 44
Data/hora: 06/06/2024 16:24:01

EXtreme ecosystem studies in the deep OCEan: Technological Developments. EXOCET/D
Provedor de dados: 5
Autores: Sarradin, Pierre-marie; Sarrazin, Jozee; Cadiou, Jean-francois; Olu, Karine; Desbruyeres, Damien; Rigaud, Vincent; Drogou, Jean-francois; Lecornu, Fabrice; Rolin, Jean-francois; Vuillemin, Renaud; Cambon-bonavita, Marie-anne; Sauter, E; Von Juterzenka, K; Boetius, A; Santos, Rs; Colaco, Ana; Pascoal, A; Oliveira, Paulo; Shillito, Bruce; Zal, F; Schultz, A; Taylor, P; Lane, D; Loke, Rob; Du Buf, H; Waldmann, C; Cormack, A; Sanfilippo, L; Masson, M.
The general objective of EXOCET/D is to develop, implement and test specific instruments aimed at exploring, describing, quantifying and monitoring biodiversity in deep-sea fragmented habitats as well as at identifying links between community structure and environmental dynamics. Onboard experimental devices will complement the approach, enabling experiments on species physiology. The EXOCET/D working fields include: video and acoustic imagery, in situ analysis of physico-chemical factors, quantitative sampling of macroand micro-organisms, in vivo experiments, integration of multidisciplinary data, implementation on European deep-submersibles and a final phase of technical and scientific validation
Tipo: Text
Palavras-chave: Deep sea; Ecosystem; Imagery; In situ analysis; Faunal sampling; Submersibles; Observatory.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2007/publication-3600.pdf

Investigation of bacterial communities within the digestive organs of the hydrothermal vent shrimp Rimicaris exoculata provide insights into holobiont geographic clustering
Provedor de dados: 5
Autores: Cowart, Dominique; Durand, Lucile; Cambon-bonavita, Marie-anne; Arnaud-haond, Sophie.
Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR). Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation detected in specific bacterial ectosymbionts. The geographic clustering of bacterial lineages within a seemingly panmictic host suggests either the presence of finer scale restriction to gene flow not yet detected in the host, horizontal transmission (environmental selection) of its endosymbionts as a consequence of unique vent geochemistry, or vertically transmitted endosymbionts that exhibit genetic...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00376/48705/49058.pdf

Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium
Provedor de dados: 5
Autores: Alain, Karine; Rolland, Sophie; Crassous, Philippe; Lesongeur, Francoise; Zbinden, Magali; Le Gall, Christian; Godfroy, Anne; Page, Antoine; Juniper, S Kim; Cambon-bonavita, Marie-anne; Duchiron, Francis; Querellou, Joel.
A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200...
Tipo: Text
Palavras-chave: Thermophile; Nitrate reducing; Lithotroph; Desulfurobacterium crinifex; Deep sea hydrothermal vent; Cell networks.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-663.pdf

Gill chamber and gut microbial communities of the hydrothermal shrimp Rimicaris chacei Williams and Rona 1986: A possible symbiosis
Provedor de dados: 5
Autores: Apremont, Vincent; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Francois, David; Pradillon, Florence; Corbari, Laure; Zbinden, Magali.
Rimicaris chacei Williams and Rona 1986, formerly named as Chorocaris chacei, is a caridean shrimp living in deep-sea hydrothermal ecosystems. This shrimp is endemic to the Mid Atlantic Ridge (MAR) and lives at the periphery of aggregates of its well-known congeneric R. exoculata Williams and Rona 1986. Contrasting with the very dense and mobile clusters formed by R. exoculata, R. chacei lives in small groups of several individuals that are not very mobile. Although devoid of the characteristic hypertrophied cephalothorax of R. exoculata, which harbors the ectosymbionts, a microbial community has also been reported in the cephalothorax of R. chacei. Previous data on morphology, behavior and isotopic values indicate a diet based on a combination of feeding...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00465/57680/59880.pdf

Cruise MoMARDREAM-Naut and other MoMAR experiments at Rainbow and Lucky Strike in Summer 2007
Provedor de dados: 5
Autores: Gaill, Françoise; Ballu, V.; Cannat, M.; Crawford, W.; Dyment, J.; Escartin, J.; Fouquet, Yves; Goslin, J.; Reverdin, G.; Sarradin, Pierre-marie; Tarits, P.; Andreani, M.; Bonnivard, E.; Bucas, Karenn; Burgaud, G.; Cambon-bonavita, Marie-anne; Cueff, Valerie; Durand, C.; Gros, O.; Hamel, G.; Henriques, M.; Hois, E.; Ildefonse, B.; Konn, Cecile; Le Bris, Nadine; Le Guyader, H.; Ravaux, J.; Shilito, B.; Toullec, J.y.; Zbinden, M.
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00385/49650/50163.pdf

Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community
Provedor de dados: 5
Autores: Durand, Lucile; Zbinden, Magali; Cueff-gauchard, Valerie; Duperron, Sebastien; Roussel, Erwan; Shillito, Bruce; Cambon-bonavita, Marie-anne.
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes,...
Tipo: Text
Palavras-chave: Deferribacteres; Midgut epibiosis; Mollicutes; Proteobacteria; Rimicaris exoculata; Starvation experiment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11142/7919.pdf

Is It First the Egg or the Shrimp? – Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development
Provedor de dados: 5
Autores: Methou, Pierre; Hernández-ávila, Ivan; Aube, Johanne; Cueff-gauchard, Valerie; Gayet, Nicolas; Amand, Louis; Shillito, Bruce; Pradillon, Florence; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is one of the most well-known and emblematic species of endemic vent fauna. Like many other species from these ecosystems, Rimicaris shrimps host important communities of chemosynthetic bacteria living in symbiosis with their host inside the cephalothorax and gut. For many of these symbiotic partners, the mode of transmission remains to be elucidated and the starting point of the symbiotic relationship is not yet defined, but could begin with the egg. In this study, we explored the proliferation of microbial communities on R. exoculata broods through embryonic development using a combination of NGS sequencing and microscopy approaches. Variations in abundance and diversity of egg microbial communities were analyzed in broods at...
Tipo: Text
Palavras-chave: Hydrothermal; Shrimp; Microbial colonization; Alvinocarididae; Egg development.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00489/60067/63352.pdf

Integrative taxonomy revisits the ontogeny and trophic niches of   Rimicaris   vent shrimps
Provedor de dados: 5
Autores: Methou, Pierre; Michel, Loic; Segonzac, Michel; Cambon-bonavita, Marie-anne; Pradillon, Florence.
Among hydrothermal vent species, Rimicaris exoculata is one of the most emblematic, hosting abundant and diverse ectosymbioses that provide most of its nutrition. Rimicaris exoculata co-occurs in dense aggregates with the much less abundant Rimicaris chacei in many Mid-Atlantic Ridge vent fields. This second shrimp also houses ectosymbiotic microorganisms but has a mixotrophic diet. Recent observations have suggested potential misidentifications between these species at their juvenile stages, which could have led to misinterpretations of their early-life ecology. Here, we confirm erroneous identification of the earliest stages and propose a new set of morphological characters unambiguously identifying juveniles of each species. On the basis of this...
Tipo: Text
Palavras-chave: Hydrothermal vents; Stable isotopes; Taxonomy; Crustaceans; Life history; Trophic shift.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00640/75227/75368.pdf

New insigths on the metabolic diversity among the epibiotic microbial communitiy of the hydrothermal shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Zbinden, M; Shillito, B; Le Bris, Nadine; De Montlaur, C; Roussel, Erwan; Guyot, F; Gaill, F; Cambon-bonavita, Marie-anne.
The Rimicaris exoculata dominates the megafauna of some of the Mid Atlantic ridge hydrothermal vent sites. This species harbors a rich community of bacterial epibionts inside its gill chamber. Literature data indicate that a single 16S rRNA phylotype dominates this epibiotic community, and is assumed to be a sulfide-oxidizing bacteria. However attempts Of Cultivation were not successful and did not allow to confirm it. The aim of our study was to test the hypothesis of sulfide oxidation in the gill chamber, by a multidisciplinary approach, using in vivo experiments at in situ pressure in the presence of sulfide, microscopic observations and a molecular survey. Morphology of microorganisms, before and after treatment, was analyzed to test the effect of...
Tipo: Text
Palavras-chave: Sulfur; Methane; Iron; Intracellular granules; Hydrothermal vent shrimp; High pressure experiments.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4563.pdf

Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers
Provedor de dados: 5
Autores: Le Bloa, Simon; Durand, Lucile; Cueff-gauchard, Valerie; Le Bars, Josiane; Taupin, Laure; Marteau, Charlotte; Bazire, Alexis; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48841/49268.pdf

Identification and Characterization of Carboxyl Esterases of Gill Chamber-Associated Microbiota in the Deep-Sea Shrimp Rimicaris exoculata by Using Functional Metagenomics
Provedor de dados: 5
Autores: Alcaide, Maria; Tchigvintsev, Anatoli; Martinez-martinez, Monica; Popovic, Ana; Reva, Oleg N.; Lafraya, Alvaro; Bargiela, Rafael; Nechitaylo, Taras Y.; Matesanz, Ruth; Cambon-bonavita, Marie-anne; Jebbar, Mohamed; Yakimov, Michail M.; Savchenko, Alexei; Golyshina, Olga V.; Yakunin, Alexander F.; Golyshin, Peter N.; Ferrer, Manuel.
The shrimp Rimicaris exoculata dominates the fauna in deep-sea hydrothermal vent sites along the Mid-Atlantic Ridge (depth, 2,320 m). Here, we identified and biochemically characterized three carboxyl esterases from microbial communities inhabiting the R. exoculata gill that were isolated by naive screens of a gill chamber metagenomic library. These proteins exhibit low to moderate identity to known esterase sequences (&lt;= 52%) and to each other (11.9 to 63.7%) and appear to have originated from unknown species or from genera of Proteobacteria related to Thiothrix/Leucothrix (MGS-RG1/RG2) and to the Rhodobacteraceae group (MGS-RG3). A library of 131 esters and 31 additional esterase/lipase preparations was used to evaluate the activity profiles of these...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00256/36721/36438.pdf

Recent population expansion and connectivity in the hydrothermal shrimp Rimicaris exoculata along the Mid-Atlantic Ridge
Provedor de dados: 5
Autores: Teixeira, Sara; Cambon-bonavita, Marie-anne; Serrao, Ester A.; Desbruyeres, Daniel; Arnaud-haond, Sophie.
Aim Deep-sea hydrothermal vents are unstable habitats that are both spatially and temporally fragmented. In vent species, a 'short-term insurance' hypothesis would lead us to expect mostly self-recruitment, limiting the loss of larvae in the deep ocean or water column and increasing genetic differentiation over the time elapsed since colonization. Alternatively, a 'long-term insurance' hypothesis would support the prediction of selection for large-scale dispersal, to ensure long-term persistence in these ephemeral habitats. The main goal of this study was to infer the spatial and temporal distribution of genetic diversity of the shrimp Rimicaris exoculata, which forms high-density local populations on hydrothermal vents along the Mid-Atlantic ridge....
Tipo: Text
Palavras-chave: Bottleneck; Deep-sea biogeography; Dispersal; Genetic diversity; Hydrothermal vents; Marine biogeography; Mid-Atlantic Ridge; Rimicaris exoculata; Spatio-temporal distribution.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14394/12350.pdf

Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna
Provedor de dados: 5
Autores: Burgaud, Gaetan; Arzur, Danielle; Durand, Lucile; Cambon-bonavita, Marie-anne; Barbier, Georges.
Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with animals, were collected. Phylogenetic analyses of 26S rRNA gene sequences revealed that the yeasts belonged to Ascomycota and Basidiomycota phyla, with the identification of several genera: Rhodotorula, Rhodosporidium, Candida, Debaryomyces and Cryptococcus. Those genera are usually isolated from deep-sea environments. To our knowledge, this is the first report of yeasts associated with deep-sea hydrothermal animals.
Tipo: Text
Palavras-chave: Yeasts; Hydrothermal vents; Fauna; 26S rRNA gene; FISH.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11704/8475.pdf

Extending the sub-sea-floor biosphere
Provedor de dados: 5
Autores: Roussel, Erwan; Cambon-bonavita, Marie-anne; Querellou, Joel; Cragg, B; Webster, G; Prieur, D; Parkes, R.
Sub-sea-floor sediments may contain two-thirds of Earth's total prokaryotic biomass. However, this has its basis in data extrapolation from ~500-meter to 4-kilometer depths, whereas the deepest documented prokaryotes are from only 842 meters. Here, we provide evidence for low concentrations of living prokaryotic cells in the deepest (1626 meters below the sea floor), oldest (111 million years old), and potentially hottest (~100°C) marine sediments investigated. These Newfoundland margin sediments also have DNA sequences related to thermophilic and/or hyperthermophilic Archaea. These form two unique clusters within Pyrococcus and Thermococcus genera, suggesting unknown, uncultured groups are present in deep, hot, marine sediments (~54° to 100°C). Sequences...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4209.pdf

Comparison of microbial communities associated with three Atlantic ultramafic hydrothermal systems
Provedor de dados: 5
Autores: Roussel, Erwan; Konn, Cecile; Charlou, Jean-luc; Donval, Jean-pierre; Fouquet, Yves; Querellou, Joel; Prieur, Daniel; Cambon-bonavita, Marie-anne.
The distribution of Archaea and methanogenic, methanotrophic and sulfate-reducing communities in three Atlantic ultramafic-hosted hydrothermal systems (Rainbow, Ashadze, Lost City) was compared using 16S rRNA gene and functional gene (mcrA, pmoA and dsrA) clone libraries. The overall archaeal community was diverse and heterogeneously distributed between the hydrothermal sites and the types of samples analyzed (seawater, hydrothermal fluid, chimney and sediment). The Lost City hydrothermal field, characterized by high alkaline warm fluids (pH&gt;11; T&lt;95 °C), harbored a singular archaeal diversity mostly composed of unaffiliated Methanosarcinales. The archaeal communities associated with the recently discovered Ashadze 1 site, one of the deepest active...
Tipo: Text
Palavras-chave: Archaea; Hydrothermal vent; Mid-Atlantic Ridge; 16S rRNA gene; Sediment; Ultramafic.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00067/17836/15573.pdf

Bacterial symbionts and mineral deposits in the branchial chamber of the hydrothermal vent shrimp Rimicaris exoculata: relationship to moult cycle
Provedor de dados: 5
Autores: Corbari, Laure; Zbinden, Magali; Cambon-bonavita, Marie-anne; Gaill, Françoise; Compere, Philippe.
The shrimp Rimicaris exoculata is considered a primary consumer that dominates the fauna of most Mid-Atlantic Ridge hydrothermal ecosystems. The shrimps harbour in their gill chamber an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The settlement and development of this ectosymbiosis was investigated using microscopy techniques (light microscopy, LM; and scanning, transmission and environmental scanning electron microscopy: SEM and ESEM, respectively) for shrimps from 2 different vent fields (Rainbow, 36 degrees 14.0' N and TAG, 26 degrees 08.0' N). The results revealed a bacterial re-colonisation after each exuviation and a development of the bacterial community in 5 steps in relation to the moult...
Tipo: Text
Palavras-chave: Iron oxides; Ectosymbiosis; Moult cycle; Shrimp; Hydrothermal vents.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4626.pdf

Influence of DNA Extraction Method, 16S rRNA Targeted Hypervariable Regions, and Sample Origin on Microbial Diversity Detected by 454 Pyrosequencing in Marine Chemosynthetic Ecosystems
Provedor de dados: 5
Autores: Cruaud, Perrine; Vigneron, Adrien; Lucchetti-miganeh, Celine; Ciron, Pierre Emmanuel; Godfroy, Anne; Cambon-bonavita, Marie-anne.
Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the results can provide inaccurate representations of the studied community compositions. Therefore, all these steps need to be taken into account carefully. Here we evaluated the effects of DNA extraction methods, targeted 16S rRNA hypervariable regions, and sample origins on the diverse microbes detected by 454 pyrosequencing in marine cold seep and hydrothermal vent sediments. To assign the reads with enough taxonomic precision, we built a database with about 2,500...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00204/31495/29907.pdf

Morphology of First Zoeal Stage of Four Genera of Alvinocaridid Shrimps from Hydrothermal Vents and Cold Seeps: Implications for Ecology, Larval Biology and Phylogeny
Provedor de dados: 5
Autores: Hernandez-avila, Ivan; Cambon-bonavita, Marie-anne; Pradillon, Florence.
Alvinocaridid shrimps are endemic species inhabiting hydrothermal vents and/or cold seeps. Although indirect evidences (genetic and lipid markers) suggest that their larval stages disperse widely and support large scale connectivity, larval life and mechanisms underlying dispersal are unknown in alvinocaridids. Here we provide for the first time detailed descriptions of the first larval stage (zoea I) of four alvinocaridid species: Rimicaris exoculata and Mirocaris fortunata from the Mid-Atlantic Ridge, Alvinocaris muricola from the Congo Basin and Nautilocaris saintlaurentae from the Western Pacific. The larvae were obtained from onboard hatching of brooding females (either at atmospheric pressure or at habitat pressure in hyperbaric chambers) and from...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00309/42011/41295.pdf

Nautilia abyssi sp nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent
Provedor de dados: 5
Autores: Alain, Karine; Callac, Nolwenn; Guegan, Marianne; Lesongeur, Francoise; Crassous, Philippe; Cambon-bonavita, Marie-anne; Querellou, Joel; Prieur, Daniel.
A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209(T), was isolated from an East Pacific Rise hydrothermal vent (13 degrees N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 mu m) with a single polar flagellum. Strain PH1209(T) grew at temperatures between 33 and 65 degrees C (optimum 60 degrees C), from pH 5.0 to 8.0 (optimum 6.0-6.5), and between 2 and 4% (w/v) NaCl (optimum 3%). Cells grew chemolithoautotrophically with H-2 as an energy source, So as an electron acceptor and CO2 as a carbon source. Strain PH1209(T) was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic: analyses...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6585.pdf

Classical and computed tomographic anatomical analyses in a not-so-cryptic Alviniconcha species complex from hydrothermal vents in the SW Pacific
Provedor de dados: 5
Autores: Laming, Sven; Hourdez, Stéphane; Cambon-bonavita, Marie-anne; Pradillon, Florence.
The chemosymbiotic gastropod Alviniconcha (Provannidae), first described in 1988, is one of the most emblematic hydrothermal-vent taxa described from the Central Indian Ridge and the Southwest (SW) Pacific. Symbiotic bacteria found in the gill of Alviniconcha are thought to be their principal source of nutrition. In the SW Pacific, species distributions for A. kojimai, A. boucheti – and to a lesser extent A. strummeri – overlap. While Alviniconcha species do not appear to truly co-exist in these highly energetic but spatially limited habitats, certain species regularly co-occur within a single vent field and in rare instances, the same edifice. Past research suggests that SW-Pacific Alviniconcha species might aggregate around fluids with distinct...
Tipo: Text
Palavras-chave: Deep sea; Chemosymbiotic; Periostracum; Gastropod; Habitat partitioning; Computed tomography; Histology; Taxonomy; 3D model.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00628/74048/73459.pdf

Phylogenetic characterization of the bacterial assemblage associated with mucous. secretions of the hydrothermal vent polychaete Paralvinella palmiformis
Provedor de dados: 5
Autores: Alain, Karine; Olagnon, Michel; Desbruyeres, Daniel; Page, Antoine; Barbier, Georges; Juniper, Kim; Querellou, Joel; Cambon-bonavita, Marie-anne.
As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. In both cases, clone libraries were largely dominated by epsilon-Proteobacteria. Phylotypes belonging to the Cytophaga-Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences were related to the taxonomic...
Tipo: Text
Palavras-chave: Proteobacteria; 16S rRNA; Microbial diversity; Phylogeny; Hydrothermal vent; Paralvinella palmiformis.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-661.pdf

Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent
Provedor de dados: 5
Autores: Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text
Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf

Evaluating sediment and water sampling methods for the estimation of deep-sea biodiversity using environmental DNA
Provedor de dados: 5
Autores: Brandt, Miriam; Pradillon, Florence; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Wincker, Patrick; Belser, Caroline; Poulain, Julie; Arnaud-haond, Sophie; Zeppilli, Daniela.
Despite representing one of the largest biomes on earth, biodiversity of the deep seafloor is still poorly known. Environmental DNA metabarcoding offers prospects for fast inventories and surveys, yet requires standardized sampling approaches and careful choice of environmental substrate. Here, we aimed to optimize the genetic assessment of prokaryote (16S), protistan (18S V4), and metazoan (18S V1–V2, COI) communities, by evaluating sampling strategies for sediment and aboveground water, deployed simultaneously at one deep-sea site. For sediment, while size-class sorting through sieving had no significant effect on total detected alpha diversity and resolved similar taxonomic compositions at the phylum level for all markers studied, it effectively...
Tipo: Text
Palavras-chave: Biodiversity; Ecological genetics; Microbial ecology; Molecular ecology.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80094/83149.pdf

Biogeographical distribution of Rimicaris exoculata resident gut epibiont communities along the Mid-Atlantic Ridge hydrothermal vent sites
Provedor de dados: 5
Autores: Durand, Lucile; Roumagnac, Marie; Cueff-gauchard, Valerie; Jan, Cyrielle; Guri, Mathieu; Tessier, Claire; Haond, Marine; Crassous, Philippe; Zbinden, Magali; Arnaud-haond, Sophie; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp which enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystems megafauna along the Mid-Atlantic Ridge, regardless of contrasted geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow/TAG/Logatchev/Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (TEM, SEM and FISH analyses). Filamentous epibionts inserted between the epithelial cells microvilli were observed on all examined samples....
Tipo: Text
Palavras-chave: Epibiont; Extreme environments; Network analyses; Phylogeography.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00288/39890/38443.pdf

Iron oxide deposits associated with the ectosymbiotic bacteria in the hydrothermal vent shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Corbari, L; Cambon-bonavita, Marie-anne; Long, G; Grandjean, F; Zbinden, M; Gaill, F; Compere, P.
The Rimicaris exoculata shrimp is considered as a primary consumer that dominates the fauna of most Mid-Atlantic Ridge ( MAR) hydrothermal ecosystems. These shrimps harbour in their gill chambers an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The structure and elemental composition of the mineral concretions associated with these bacteria have been investigated by using LM, ESEM, TEM STEM and EDX microanalyses. The nature of the iron oxides in shrimps obtained from the Rainbow vent field has also been determined by Mossbauer spectroscopy. This multidisciplinary approach has revealed that the three layers of mineral crust in the Rimicaris exoculata shrimps consist of large concretions formed by...
Tipo: Text
Palavras-chave: Mid atlantic ridge; Deep sea; Microbial communities; Oxidizing bacteria; Trophic ecology; Ferrous ions; Fe oxides; Ferrihydrite; Biomineralization; Minerals.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4702.pdf

Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Auguste, M.; Mestre, N. C.; Rocha, T. L.; Cardoso, C.; Cueff-gauchard, Valerie; Le Bloa, Simon; Cambon-bonavita, Marie-anne; Shillito, B.; Zbinden, M.; Ravaux, J.; Bebianno, M. J.
In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30 MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630 m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues...
Tipo: Text
Palavras-chave: Deep-sea mining; Hydrothermal vents; Biomarkers; Ipocamp; Copper; TAG.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00323/43465/42869.pdf

Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text
Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf

"Candidatus Desulfobulbus rimicarensis," an Uncultivated Deltaproteobacterial Epibiont from the Deep-Sea Hydrothermal Vent Shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Jiang, Lijing; Liu, Xuewen; Dong, Chunming; Huang, Zhaobin; Cambon-bonavita, Marie-anne; Alain, Karine; Gu, Li; Wang, Shasha; Shao, Zongze.
The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. exoculata from the relatively unexplored South Mid-Atlantic Ridge. A nearly complete genome of a Deltaproteobacteria epibiont was binned from the assembled metagenome. Whole-genome phylogenetic analysis reveals that it is affiliated with the genus Desulfobulbus, representing a potential novel species for which the name "Candidatus Desulfobulbus rimicarensis" is proposed. Genomic and transcriptomic analyses reveal that this bacterium utilizes...
Tipo: Text
Palavras-chave: Deltaproteobacteria; Rimicaris exoculata; Wood-Ljungdahl pathway; Epibiont; Sulfur disproportionation.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00624/73600/76007.pdf

Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea Alvinocarid shrimp Rimicaris exoculata gut
Provedor de dados: 5
Autores: Zbinden, Magali; Cambon-bonavita, Marie-anne.
The phylogenetic diversity of the gut microbial population of the hydrothermal shrimp Rimicaris exoculata was determined. The presence of micro-organisms in the hindgut of the shrimp was determined, and their morphology illustrated for the first time by transmission electron microscopy. DNA was extracted from the fore-, mid- and hindgut of shrimps collected at the Rainbow site, at the Mid-Atlantic Ridge, and the sequences of the small-subunit rRNA (16S rDNA) were analyzed. Seven different bacterial phylotypes were identified from the 90 clones screened. The sequences were affiliated to three major groups: epsilon-Proteobacteria (48,8%), Entomoplasmatales (23.3%) and representatives of the new phylum Deferribacteres (26.6%). These results show that the...
Tipo: Text
Palavras-chave: Metal bioremediation; Indigenous gut microllora; Genetic diversity; Shrimp; Hydrothermal vent.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-778.pdf

Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin
Provedor de dados: 5
Autores: Cambon-bonavita, Marie-anne; Nadalig, Thierry; Roussel, Erwan; Delage, Eloise; Duperron, Sebastien; Caprais, Jean-claude; Boetius, A.; Sibuet, Myriam.
A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups...
Tipo: Text
Palavras-chave: Microbial phylogeny; Faunal assemblage; Cold seep; Chemosynthetic ecosystems; AOM; REGAB.
Ano: 2009
URL: https://archimer.ifremer.fr/doc/2009/publication-7298.pdf

Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise
Provedor de dados: 5
Autores: Moussard, Hélène; Corre, Erwan; Cambon-bonavita, Marie-anne; Fouquet, Yves; Jeanthon, Christian.
Rapid growth of microbial sulphur mats have repeatedly been observed during oceanographic cruises to various deep-sea hydrothermal vent sites. The microorganisms involved in the mat formation have not been phylogenetically characterized, although the production of morphologically similar sulphur filaments by a Arcobacter strain coastal marine has been documented. An in situ collector deployed for 5 days at the 13 degrees N deep-sea hydrothermal vent site on the East Pacific Rise (EPR) was rapidly colonized by a filamentous microbial mat. Microscopic and chemical analyses revealed that the mat consisted of a network of microorganisms embedded in a mucous sulphur-rich matrix. Molecular surveys based on 16S rRNA gene and aclB genes placed all the...
Tipo: Text
Palavras-chave: FISH; Arcobacter; Epsilonproteobacteria; 16S rRNA gene; Biofilm; Deep sea hydrothermal vent.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2110.pdf

Rimicaris exoculata: biology and ecology of a shrimp from deep-sea hydrothermal vents associated with ectosymbiotic bacteria
Provedor de dados: 5
Autores: Zbinden, M; Cambon-bonavita, Marie-anne.
Rimicaris exoculata, the ‘blind shrimp,’ is the most abundant species living on active hydrothermal edifices at deep-sea vents of the Mid-Atlantic Ridge. Its unusually enlarged branchial chamber houses a dense ectosymbiotic community of chemoautotrophic bacteria. Long debated, shrimp nutrition has been proven to be a kind of osmotrophy, whereby small organic molecules produced by the symbionts pass through the integument of the shrimp directly into the circulatory system, rather than through the digestive system. The broad phylogenetic and metabolic diversity of this epibiotic community suggests a highly flexible and adjustable microbial consortium, adapted to the chemically contrasting environments inhabited by the shrimp. To cope with the highly...
Tipo: Text
Palavras-chave: Alvinocaridid shrimp; Chemoautotrophic symbiont; Deep-sea; Epibiosis; Holobiont; Hydrothermal vents; Mid-Atlantic Ridge; Rimicaris exoculata.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00655/76681/77812.pdf

Comparative Study of Guaymas Basin Microbiomes: Cold Seeps vs. Hydrothermal Vents Sediments
Provedor de dados: 5
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both...
Tipo: Text
Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf

Microbial Communities of the Shallow-Water Hydrothermal Vent Near Naples, Italy, and Chemosynthetic Symbionts Associated With a Free-Living Marine Nematode
Provedor de dados: 5
Autores: Bellec, Laure; Cambon-bonavita, Marie-anne; Durand, Lucile; Aube, Johanne; Gayet, Nicolas; Sandulli, Roberto; Brandily, Christophe; Zeppilli, Daniela.
Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the...
Tipo: Text
Palavras-chave: Nematode; Shallow-water hydrothermal vent; Sulfur-oxidizing bacteria; Iron cycle; Zetaproteobacteria.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75544/76449.pdf

Caloranaerobacter azorensis gen. nov., sp nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent
Provedor de dados: 5
Autores: Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The...
Tipo: Text
Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf

Caminicella sporogenes gen. nov., sp nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent
Provedor de dados: 5
Autores: Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne.
A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex...
Tipo: Text
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf

Pyrococcus CH1, an obligate piezophilic hyperthermophile: extending the upper pressure-temperature limits for life
Provedor de dados: 5
Autores: Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel.
A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic...
Tipo: Text
Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf

Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp
Provedor de dados: 5
Autores: Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie.
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at...
Tipo: Text
Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf

Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
Provedor de dados: 5
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers...
Tipo: Text
Palavras-chave: Microbial communities; Pyrosequencing; MBG-D; ANME; Faunal assemblages; Vesicomyidae; Siboglinidae; Microbial mat.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00288/39889/38434.pdf

Ecology and trophic role of Oncholaimus dyvae sp. nov. (Nematoda: Oncholaimidae) from the lucky strike hydrothermal vent field (Mid-Atlantic Ridge)
Provedor de dados: 5
Autores: Zeppilli, Daniela; Bellec, Laure; Cambon-bonavita, Marie-anne; Decraemer, Wilfrida; Fontaneto, Diego; Fuchs, Sandra; Gayet, Nicolas; Mandon, Perrine; Michel, Loic; Portail, Marie; Smol, Nic; Sørensen, Martin V.; Vanreusel, Ann; Sarrazin, Jozee.
Background Nematodes are an important component of deep-sea hydrothermal vent communities, but only few nematode species are able to cope to the harsh conditions of the most active vent sites. The genus Oncholaimus is known to tolerate extreme geothermal conditions and high sulphide concentrations in shallow water hydrothermal vents, but it was only occasionally reported in deep-sea vents. In this study, we performed morphological, genetic and ecological investigations (including feeding strategies) on an abundant species of Oncholaimus recently discovered at Lucky strike vent field on the Mid-Atlantic Ridge at 1700 m water depth. Results We described this species as Oncholaimus dyvae sp. nov.. This new species differs from all other members of the genus...
Tipo: Text
Palavras-chave: Oncholaimus dyvae sp. nov.; Hydrothermal vents; Deep sea; Carbon and nitrogen isotopic ratios.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00508/61985/66097.pdf

The gill chamber epibiosis of deep-sea shrimp Rimicaris exoculata: an in-depth metagenomic investigation and discovery of Zetaproteobacteria
Provedor de dados: 5
Autores: Jan, Cyrielle; Petersen, Jillian M.; Werner, Johannes; Teeling, Hanno; Huang, Sixing; Gloeckner, Frank Oliver; Golyshina, Olga V.; Dubilier, Nicole; Golyshin, Peter N.; Jebbar, Mohamed; Cambon-bonavita, Marie-anne.
The gill chamber of deep-sea hydrothermal vent shrimp Rimicaris exoculata hosts a dense community of epibiotic bacteria dominated by filamentous Epsilonproteobacteria and Gammaproteobacteria. Using metagenomics on shrimp from the Rainbow hydrothermal vent field, we showed that both epibiont groups have the potential to grow autotrophically and oxidize reduced sulfur compounds or hydrogen with oxygen or nitrate. For carbon fixation, the Epsilonproteobacteria use the reductive tricarboxylic acid cycle, whereas the Gammaproteobacteria use the Calvin–Benson–Bassham cycle. Only the epsilonproteobacterial epibionts had the genes necessary for producing ammonium. This ability likely minimizes direct competition between epibionts and also broadens the spectrum of...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00177/28874/28622.pdf

Bacterial communities and syntrophic associations involved in anaerobic oxidation of methane process of the Sonora Margin cold seeps, Guaymas Basin
Provedor de dados: 5
Autores: Vigneron, Adrien; Cruaud, Perrine; Pignet, Patricia; Caprais, Jean-claude; Gayet, Nicolas; Cambon-bonavita, Marie-anne; Godfroy, Anne; Toffin, Laurent.
The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previous study on the same samples identified anaerobic oxidation of methane (AOM) as the potential dominant archaeal process in these Sonora Margin sediments, probably catalysed by three clades of archaeal anaerobic methanotrophs (ANME-1, ANME-2 and ANME-3) associated with bacterial syntrophs. In this study, molecular surveys and microscopic observations investigating the diversity of Bacteria involved in AOM...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00168/27921/27279.pdf

A Nematode of the Mid-Atlantic Ridge Hydrothermal Vents Harbors a Possible Symbiotic Relationship
Provedor de dados: 5
Autores: Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela.
Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and...
Tipo: Text
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf

Niche partitioning in the Rimicaris exoculata holobiont: the case of the first symbiotic Zetaproteobacteria
Provedor de dados: 5
Autores: Cambon-bonavita, Marie-anne; Aubé, Johanne; Cueff-gauchard, Valerie; Reveillaud, Julie.
Background Free-living and symbiotic chemosynthetic microbial communities support primary production and higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata, which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax. The dominant bacteria present are from the taxonomic groups Campylobacteria, Desulfobulbia (formerly Deltaproteobacteria), Alphaproteobacteria, Gammaproteobacteria, and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria. This epibiotic consortium uses iron, sulfide, methane, and hydrogen as energy sources. Here, we generated shotgun metagenomes from Rimicaris exoculata cephalothoracic epibiotic communities to reconstruct...
Tipo: Text
Palavras-chave: Niche partitioning; Rimicaris; Metagenome-assembled genomes; Zetaproteobacteria; Holobiont; Hydrothermal vents.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80089/83128.pdf