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Data/hora: 12/06/2024 10:53:24

Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
Provedor de dados: 56
Autores: Aragão,T.C.F.R.; Souza,P.A.S.; Uchôa,A.F.; Costa,I.R.; Bloch Jr.,C.; Campos,F.A.P.
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Mandacaru; Cereus jamacaru; Cactin; 2S albumins; Methionine-rich protein.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005

Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
Provedor de dados: 56
Autores: Uchoa,A.F.; Souza,P.A.S.; Zarate,R.M.L.; Gomes-Filho,E.; Campos,F.A.P.
We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Prickly-pear; Opuntia ficus-indica; Seed proteins; 2S albumins.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005