Consulta: creatorFacets:"Campos,Francisco de Assis Paiva"
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Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)
Provedor de dados: 52
Autores: Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva.
We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005