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Radiation-induced Changes in the Electrophoretic Profile of Serum Albumin
Provedor de dados: 52
Autores: Lima,Celso Vieira; Campos,Tarcisio Passos Ribeiro.
ABSTRACT Albumin protein profiles were investigated in electrophoresis system in relation to the whole body exposition to the radiation. Two groups of rats Wistar were set up as the control (CG) and the irradiated one (IG). The IG was exposed to Co-60 at a dose of 5 Gy. After a 72-hour exposition, 300 μL of blood was collected in the inferior vena cava, renal, jugular, hepatic, and pulmonary veins and the serum separated. The albumin protein was identified by vertical electrophoresis in acrylamide Commassi blue or silver stained. The calibration procedure was applied to albumin samples with well-known concentrations. The mathematical correlation was developed involving electrophoretic parameters of band intensities and sizes from gel representation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum protein; Rats; Radiation; Albumin; Electrophoresis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100444