Consulta: creatorFacets:"Capelozzi,V.L."
Registros recuperados: 13
Data/hora: 07/06/2024 04:51:29

Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Provedor de dados: 56
Autores: Parra,E.R.; Pincelli,M.S.; Teodoro,W.R.; Velosa,A.P.P.; Martins,V.; Rangel,M.P.; Barbas-Filho,J.V.; Capelozzi,V.L.
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BHT experimental model; Usual interstitial pneumonia; Alveolar epithelial cell apoptosis; Telomerase activity; Collagen V; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700567

Detection of sputum cofilin-1 as indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; Antonangelo,L.; Acencio,M.M.P.; Faria,C.S.; de Sá,V.K.; Leão,P.S.; Farhat,C.; Fabro,A.T.; Longatto Filho,A.; Reis,R.M.; Takagaki,T.; Capelozzi,V.L.
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P&lt;0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer; Cofilin-1; Sputum; Liquid-biopsy; Metastases; Biomarker.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800603

Role of the extracellular matrix in variations of invasive pathways in lung cancers
Provedor de dados: 56
Autores: de Sá,V.K.; Carvalho,L.; Gomes,A.; Alarcão,A.; Silva,M.R.; Couceiro,P.; Sousa,V.; Soares,F.A.; Capelozzi,V.L.
Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer E-cadherin TGF-β HAS-1; HAS-2 and HAS-3 Hyal-1 and Hyal-3 Immunohistochemistry Prognosis and morphometry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100021

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557

Lymphatic fluctuation in the parenchymal remodeling stage of acute interstitial pneumonia, organizing pneumonia, nonspecific interstitial pneumonia and idiopathic pulmonary fibrosis
Provedor de dados: 56
Autores: Parra,E.R.; Araujo,C.A.L.; Lombardi,J.G.; Ab’Saber,A.M.; Carvalho,C.R.R.; Kairalla,R.A.; Capelozzi,V.L.
Because the superficial lymphatics in the lungs are distributed in the subpleural, interlobular and peribroncovascular interstitium, lymphatic impairment may occur in the lungs of patients with idiopathic interstitial pneumonias (IIPs) and increase their severity. We investigated the distribution of lymphatics in different remodeling stages of IIPs by immunohistochemistry using the D2-40 antibody. Pulmonary tissue was obtained from 69 patients with acute interstitial pneumonia/diffuse alveolar damage (AIP/DAD, N = 24), cryptogenic organizing pneumonia/organizing pneumonia (COP/OP, N = 6), nonspecific interstitial pneumonia (NSIP/NSIP, N = 20), and idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP, N = 19). D2-40+ lymphatic in the lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphangiogenesis; Lymphatic vessel; Idiopathic interstitial pneumonias; Lung remodeling; Immunohistochemistry; Morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500013

Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
Provedor de dados: 56
Autores: Sá,V.K. de; Rocha,T.P.; Moreira,AL.; Soares,F.A.; Takagaki,T.; Carvalho,L.; Nicholson,A.G.; Capelozzi,V.L.
We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidases and hyaluronan synthases; Lung cancer; Pre-neoplastic lung/bronchial lesions; Immunohistochemistry; Morphometry; Prognosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101039

Immunohistochemical detection of virus through its nuclear cytopathic effect in idiopathic interstitial pneumonia other than acute exacerbation
Provedor de dados: 56
Autores: Santos,G.C. dos; Parra,E.R.; Stegun,F.W.; Cirqueira,C.S.; Capelozzi,V.L.
Idiopathic interstitial pneumonias include complex diseases that have a strong interaction between genetic makeup and environmental factors. However, in many cases, no infectious agent can be demonstrated, and these clinical diseases rapidly progress to death. Theoretically, idiopathic interstitial pneumonias could be caused by the Epstein-Barr virus, cytomegalovirus, adenovirus, hepatitis C virus, respiratory syncytial virus, and herpesvirus, which may be present in such small amounts or such configuration that routine histopathological analysis or viral culture techniques cannot detect them. To test the hypothesis that immunohistochemistry provides more accurate results than the mere histological demonstration of viral inclusions, this method was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrosing interstitial pneumonia; Viral infections; Immunohistochemistry; Tissue microarray.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100985

Identification of rounded atelectasis in workers exposed to asbestos by contrast helical computed tomography
Provedor de dados: 56
Autores: Terra-Filho,M.; Kavakama,J.; Bagatin,E.; Capelozzi,V.L.; Nery,L.E.; Tavares,R.
Rounded atelectasis (RA) is a benign and unusual form of subpleural lung collapse that has been described mostly in asbestos-exposed workers. This form of atelectasis manifests as a lung nodule and can be confused with bronchogenic carcinoma upon conventional radiologic examination. The objective of the present study was to evaluate the variation in contrast uptake in computed tomography for the identification of asbestos-related RA in Brazil. Between January 1998 and December 2000, high-resolution computed tomography (HRCT) was performed in 1658 asbestos-exposed workers. The diagnosis was made in nine patients based on a history of prior asbestos exposure, the presence of characteristic (HRCT) findings and lesions unchanged in size over 2 years or more....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rounded atelectasis; Asbestos; Helical computed tomography; Asbestos-exposed workers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000010

Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis
Provedor de dados: 56
Autores: Colombo,N.B.R.; Rangel,M.P.; Martins,V.; Hage,M.; Gelain,D.P.; Barbeiro,D.F.; Grisolia,C.K.; Parra,E.R.; Capelozzi,V.L.
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi; Caryocar brasiliense Camb; Oxidative stress; Lung cancer; Urethane; Molecular biology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900852

Morphometric evaluation of nitric oxide synthase isoforms and their cytokine regulators predict pulmonary dysfunction and survival in systemic sclerosis
Provedor de dados: 56
Autores: Parra,E.R.; Aguiar Junior,A.C.; Silva,L.O.; Souza,H.S.P.; Espinoza,J.D.; Capelozzi,V.L.
Because histopathological changes in the lungs of patients with systemic sclerosis (SSc) are consistent with alveolar and vessel cell damage, we presume that this interaction can be characterized by analyzing the expression of proteins regulating nitric oxide (NO) and plasminogen activator inhibitor-1 (PAI-1) synthesis. To validate the importance of alveolar-vascular interactions and to explore the quantitative relationship between these factors and other clinical data, we studied these markers in 23 cases of SSc nonspecific interstitial pneumonia (SSc-NSIP). We used immunohistochemistry and morphometry to evaluate the amount of cells in alveolar septa and vessels staining for NO synthase (NOS) and PAI-1, and the outcomes of our study were cellular and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic sclerosis; Nitric oxide synthase isoforms; Cytokines; Morphometry; Pulmonary function tests and survival.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000881

Inactivation of capsaicin-sensitive nerves reduces pulmonary remodeling in guinea pigs with chronic allergic pulmonary inflammation
Provedor de dados: 56
Autores: Prado,C.M.; da Rocha,G.Z.; Leick-Maldonado,E.A.; Starling,C.M.; Capelozzi,V.L.; Martins,M.A.; Tibério,I.F.L.C.
Pulmonary remodeling is an important feature of asthma physiopathology that can contribute to irreversible changes in lung function. Although neurokinins influence lung inflammation, their exact role in the extracellular matrix (ECM) remodeling remains to be determined. Our objective was to investigate whether inactivation of capsaicin-sensitive nerves modulates pulmonary ECM remodeling in animals with chronic lung inflammation. After 14 days of capsaicin (50 mg/kg, sc) or vehicle administration, male Hartley guinea pigs weighing 250-300 g were submitted to seven inhalations of increasing doses of ovalbumin (1, 2.5, and 5 mg/mL) or saline for 4 weeks. Seventy-two hours after the seventh inhalation, animals were anesthetized and mechanically ventilated and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Elastic fibers; Capsaicin; Chronic lung inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200007

p53 immunostaining is correlated with reduced survival and is not correlated with gene mutations in resected pulmonary large cell carcinomas
Provedor de dados: 56
Autores: Massoni Neto,L.M.; Bianchi,C.P.; Ab'Saber,A.M.; Parra,E.R.; Takagaki,T.; Pereira,J.C.; Soares,F.A.; Leite,K.; Capelozzi,V.L.
Malignancy of pulmonary large cell carcinomas (LCC) increases from classic LCC through LCC with neuroendocrine morphology (LCCNM) to large cell neuroendocrine carcinomas (LCNEC). However, the histological classification has sometimes proved to be difficult. Because the malignancy of LCC is highly dependent on proteins with functions in the cell cycle, DNA repair, and apoptosis, p53 has been targeted as a potentially useful biological marker. p53 mutations in lung cancers have been shown to result in expression and protein expression also occurs in the absence of mutations. To validate the importance of both p53 protein expression (by immunostaining) and p53 gene mutations in lung LCC (by PCR-single strand conformational polymorphism analysis of exons 5, 6,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Large cell carcinoma; P53; Genetics; Molecular biology; Lung cancer; Mutation analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800004

Vascular dysfunction by myofibroblast activation in patients with idiopathic pulmonary fibrosis and prognostic significance
Provedor de dados: 56
Autores: Parra,E.R.; Falzoni,R.; Capelozzi,V.L.
In this study, we demonstrated the importance of telomerase protein expression and determined the relationships among telomerase, endothelin-1 (ET-1) and myofibroblasts during early and late remodeling of parenchymal and vascular areas in usual interstitial pneumonia (UIP) using 27 surgical lung biopsies from patients with idiopathic pulmonary fibrosis (IPF). Telomerase+, myofibroblasts α-SMA+, smooth muscle cells caldesmon+, endothelium ET-1+ cellularity, and fibrosis severity were evaluated in 30 fields covering normal lung parenchyma, minimal fibrosis (fibroblastic foci), severe (mural) fibrosis, and vascular areas of UIP by the point-counting technique and a semiquantitative score. The impact of these markers was determined in pulmonary functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Idiopathic pulmonary fibrosis; Vascular activity; Immunohistochemistry; Survival.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700015