Consulta: creatorFacets:"Caprais, Marie-paule"
Registros recuperados: 25
Data/hora: 01/06/2024 11:52:01

Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish
Provedor de dados: 5
Autores: Loisy, Fabienne; Atmar, R; Le Saux, Jean-claude; Cohen, J; Caprais, Marie-paule; Pommepuy, Monique; Le Guyader, Soizick.
Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue)...
Tipo: Text
Palavras-chave: Seawater; Viral diseases; Bioaccumulation; Depuration; Contamination; Model; Pancreas; Regression analysis; Shellfish; Phages; Virus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1233.pdf

Etude de l'activité enzymatique pour la détection des entérobactéries en eau de mer
Provedor de dados: 5
Autores: Melikechi, H; Pommepuy, Monique; Caprais, Marie-paule.
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00041/15207/12535.pdf

Validity of Escherichia coli, enterovirus, and F-specific RNA bacteriophages as indicators of viral shellfish contamination
Provedor de dados: 5
Autores: Miossec, Laurence; Le Guyader, Soizick; Pelletier, Dominique; Haugarreau, Larissa; Caprais, Marie-paule; Pommepuy, Monique.
The sanitary classification of harvesting areas for bivalve mollusks in France is based on the level of Escherichia Coli contamination detected in shellfish meat, as defined in EC Directive 91/492 EEC. However, outbreaks of gastroenteritis or hepatitis after consumption of shellfish meeting current bacteriological standards suggest that E. coli is a poor indicator of viral contamination. The purpose of this study was to assess the adequacy of enterovirus and F-specific RNA bacteriophages as new indicators of human enteric viruses. Shellfish were sampled over a 37-mo period to characterize microbial contamination in two coastal areas subjected to different sewage contamination inputs. Contamination by E. coli, F-specific RNA bacteriophages (F+ RNA) and...
Tipo: Text
Palavras-chave: Indicator; Escherichia coli; F specific RNA bacteriophages; Enteric viruses; Viral contamination; Shellfish.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/00000/10006/7794.pdf

Mise en place d'éléments permettant le fonctionnement d'organismes stressés dans un écosystème pertubé - 2ème partie : rôle et expression du gène rpoS chez Escherichia Coli en situation de stress
Provedor de dados: 5
Autores: Wieliczko, Etienne; Gourmelon, Michele; Dupray, Elisabeth; Derrien, A.; Caprais, Marie-paule; Pommepuy, Monique.
D'un point de vue purement écologique, le milieu marin est particulièrement agressif pour les bactéries d'origine entérique. Jusqu'à la fin des années soixante dix, il était généralement admis que ces bactéries pathogènes étaient détruites en quelques heures dans l'eau de mer, ou selon l'espèce en quelques jours. On parlait alors du "pouvoir auto-épurateur" de l'eau de mer. Depuis des travaux plus récents ont démontré la persistance de microorganismes dans les zones littorales. En fait, la survie en mer des bactéries d'origine fécale dans le milieu marin dépend de deux grands facteurs : la qualité de l'eau ou du sédiment permettant aux bactéries de mettre en oeuvre plus ou moins facilement des mécanismes de "résistance", et leur historique avant leur rejet...
Tipo: Text
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00105/21630/19210.pdf

Development of microbial and chemical MST tools to identify the origin of the faecal pollution in bathing and shellfish harvesting waters in France
Provedor de dados: 5
Autores: Gourmelon, Michele; Caprais, Marie-paule; Mieszkin, Sophie; Marti, Romain; Wery, Nathalie; Jarde, E.; Derrien, Marc; Jadas-hecart, A.; Communal, P. Y.; Jaffrezic, A.; Pourcher, A. M.
The microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. An efficient MST (Microbial Source Tracking) toolbox consisting of several host-specific markers would therefore be valuable for identifying the origin of the faecal pollution in the environment and thus for effective resource management and remediation. In this multidisciplinary study, after having tested some MST markers on faecal samples, we compared a selection of 17 parameters corresponding to chemical (steroid ratios, caffeine, and synthetic compounds), bacterial (host-specific Bacteroidales, Lactobacillus amylovorus and Bifidobacterium adolescentis) and viral (genotypes I-IV of F-specific bacteriophages, FRNAPH) markers on...
Tipo: Text
Palavras-chave: Microbial source tracking; Faecal pollution; Bacteroidales; Bifidobacterium adolescentis; Lactobacillus; FRNA bacteriophages; Steroids; Caffeine.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00016/12710/9690.pdf

Identification de l'origine de la contamination observée en baie de La Baule : Première partie de l'étude
Provedor de dados: 5
Autores: Caprais, Marie-paule; Gourmelon, Michele; Le Mennec, Cecile; Joubrel, Rozenn.
Afin d'améliorer la qualité sanitaire des coquillages du secteur La Baule - Le Pouliguen, la Communauté d'Agglomération CAP Atlantique a mis en place un programme d'étude pour identifier les sources de pollution sur ce secteur et initier des actions permettant de limiter les apports d'origine fécale aux coquillages. Dans le cadre de cette présente étude, de juin 2006 à décembre 2007, deux méthodes d'identification de l'origine de la contamination fécale en cours de développement au laboratoire de microbiologie d'Ifremer de Brest, ont été appliquées sur des lots de coquillages (n=87 ; 6 sites) et des échantillons d'eaux (n=40 ; 7 localisations). La première méthode consistait à rechercher par PCR conventionnelle les gènes codant les ARN ribosomiques (ARNr)...
Tipo: Text
Palavras-chave: Bacteroidales; Bactériophages F +ARN spécifiques; Marqueurs de contamination fécale; E. coli; Génotypage; PCR conventionnelle; Coquillage; Littoral.
Ano: 2008
URL: https://archimer.ifremer.fr/doc/00622/73452/72691.pdf

Potential of F-specific RNA bacteriophages to discriminate sources of faecal pollution in French shellfish
Provedor de dados: 5
Autores: Caprais, Marie-paule; Le Mennec, Cecile; Pommepuy, Monique; Le Saux, Jean-claude; Gourmelon, Michele.
In Europe, shellfish harvesting areas are classified according European Directive EC N°854/2004, using classic faecal indicator levels (Escherichia coli). A better indicator is needed, however, because shellfish may contain other human pathogens. Other indicator organisms have therefore been evaluated and, among these, coliphages meet most of the requirements to serve as indicators and models for enteroviruses. In order to evaluate the capacity of coliphages as indicators, a total of 764 shellfish were sampled in 2004 from 70 shellfish harvesting areas monitored by the Ifremer microbiological surveillance network (REMI, http://www.ifremer.fr/envlit/), and tested for F-specific RNA bacteriophages (FRNAPH). Two species of bivalve mollusc were collected...
Tipo: Text
Palavras-chave: F-specific RNA bacteriophage; Shellfish; Faecal pollution; Discrimination.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00066/17749/15271.pdf

Investigating an avian source of shellfish faecal contamination in the Thau lagoon (the Mediterranean, France)
Provedor de dados: 5
Autores: Derolez, Valerie; Serais, Ophelie; Caprais, Marie-paule; Le Saux, Jean-claude; Messiaen, Gregory.
Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather.Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were...
Tipo: Text
Palavras-chave: Escherichia coli; Origine aviaire; Coquillage; Contamination fécale; Étang de Thau; Oiseau de mer; Bactériophage; Dépistage de la source microbienne; Temps sec; Escherichia coli; Avian source; Shellfish; Faecal contamination; Thau lagoon; Seabird; Bacteriophage; Microbial source tracking; Dry weather.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00025/13593/10659.pdf

Identification of 129 Micrococcaceae strains isolated from food of animal origin
Provedor de dados: 5
Autores: Delarras, C; Guichaoua, C.; Caprais, Marie-paule.
129 strains of Micrococcaceae were isolated from food of animal origin (minced meat, cakes with confectioner's custard) on Baird-Parker medium. 120 were Staphylococcus and 9 Micrococcus, according to the ID 32 STAPH-System (1989). The results were analyzed using a computer program. 60% of these 120 Staphylococcus strains of animal origin were coagulase positive S. aureus (aurease+, Rapidec Staph technique). 46% were resistant to novobiocin (5 microg/ml), 13 to 44% were identified with negative discordant tests using this micromethod. 44% produced no acetoin, 35% had no urease and 15% no arginine dihydrolase. 48 Staphylococcus strains (40%) were identified as human coagulase negative Staphylococcus species (strains: 39; species: 9) or as animal species...
Tipo: Text
Palavras-chave: Staphylococcus; Nitrofurantoin; Aurease; ID 32 STAPH; Numerical identification.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00071/18273/15838.pdf

Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study
Provedor de dados: 5
Autores: Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie.
An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages...
Tipo: Text
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf

Evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in french estuaries
Provedor de dados: 5
Autores: Gourmelon, Michele; Caprais, Marie-paule; Segura, Raphael; Le Mennec, Cecile; Lozach, Solen; Piriou, Jean-yves; Rince, A.
In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the Bacteroidales markers was evaluated on human and animal (bovine, pig, sheep, and bird) feces. Two human-specific markers (HF183 and HF134), one ruminant-specific marker (CF193'), and one pig-specific marker (PF163) showed a high level of specificity (&gt;90%). However, the data suggest that the proposed ruminant-specific CF128 marker would be better described as an animal marker, as it was observed in all bovine and sheep feces and 96% of pig feces. F RNA...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3535.pdf

Contamination virale et indicateurs du risque (Programme Liteau)
Provedor de dados: 5
Autores: Pommepuy, Monique; Caprais, Marie-paule; Le Mennec, Cecile; Parnaudeau, Sylvain; Le Guyader, Francoise; Camus, Patrick; Dimeet, Joel; Treguier, Cathy; Bon, Fabienne; Pothier, Pierre; Kohli E, Evelyne; Brachet, R.; Flahault, A.; Sarrette, B.; Vilagines, P.
Le développement des activités humaines dans les régions littorales entraîne le rejet de germes pathogènes d'origine fécale dans l'environnement estuarien et marin. L'amélioration des traitements d'épuration, mais aussi la mise en place de normes bactériologiques pour surveiller les zones de production conchylicole, ont entraîné une diminution des épidémies liées à la consommation de coquillages. Ainsi, à l'heure actuelle, même si l'origine de ces gastro-entérites n'est pas élucidée dans sa globalité (rôle probable des vibrios ou d'autres bactéries), les virus semblent jouer un rôle important et leur implication té clairement établie dans les phénomènes épidémiques (Miossec et Vaillant, 2001) Les principaux virus humains susceptibles d'être détectés dans...
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15549/12936.pdf

Etude de la qualité du milieu marin littoral en Martinique (Baie de Fort de France)
Provedor de dados: 5
Autores: Breton, Marguerite; Caprais, Marie-paule; Derrien, A.; Douillet, P; Dubois, E; Dupray, E; Gourmelon, Michele; Guillerm, Denise; Kempf, Marc; Lazure, P.; Le Guyader, Soizick; Le Menn, Remi; Menard, Dominique; Pommepuy, Monique; Salomon, Jean-claude.
Les départements et territoires d'outre mer sont généralement l'objet d'enjeux économiques importants, et de conflits d'activités liés au développement touristique, aquacole, agricole et industriel. Les études font généralement état d'une lente dégradation de l'environnement marin avec diminution des coraux et de la faune associée, au profit d'une augmentation de la couverture algale. On constate parfois un envasement ou un ensablement des sites, la présence dans l'eau de polluants organiques, dont des pesticides, des métaux ainsi que des microorganismes d'origine fécale. En Martinique, ce dernier aspect, lié à l'assainissement des zones rurales, urbaines et touristiques est actuellement considéré comme une préoccupation majeure pour les prochaines années....
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00132/24303/22301.pdf

Des micro-organismes et des composés chimiques pour identifier les sources de contamination fécale : étude de leur persistance en microcosmes et de leur présence dans les eaux à l'échelle d'un bassin versant
Provedor de dados: 5
Autores: Pourcher, Anne-marie; Jarde, Emilie; Jeanneau, Laurent; Solecki, Olivia; Jadas-hecart, Alain; Caprais, Marie-paule; Durand, Gaëlle; Gourmelon, Michele.
La dégradation de la qualité des eaux par la pollution microbiologique représente un problème majeur de santé publique, notamment dans les eaux ou s'exercent des activité humaines. Afin de définir les actions prioritaires à mener, une meilleure connaissance de l'origine des pollution fécales est nécessaire. Cet article nous présente le développement d'une méthode innovante utilisant des marqueurs chimiques et microbiologiques permettant de différencier trois sources majeures de contamination des eaux de baignade en Bretagne: les déjections humaines, bovines et porcines.
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00315/42604/41980.pdf

Microbial impact of small tributaries on water and shellfish quality in shallow coastal areas
Provedor de dados: 5
Autores: Riou, Philippe; Le Saux, Jean-claude; Dumas, Franck; Caprais, Marie-paule; Le Guyader, Soizick; Pommepuy, Monique.
This study aimed to investigate the impact of small tributaries on seawater and shellfish quality in coastal area subjected to brief episodes leading to fecal contamination. Escherichia coli and F-RNA-specific bacteriophages were selected as fecal indicators and astroviruses were chosen as being representative of pathogens in the human population during winter viral epidemics. A two-dimensional hydrodynamic model was built to simulate the current and dispersion in the model domain, which includes areas uncovered at low tide. The model also includes decay rates to simulate microorganism behavior and assess the influence of fecal input on shellfish quality. The originality lies in the fact that specific features of the study area were considered. Modeling...
Tipo: Text
Palavras-chave: Shellfish; Astrovirus; F RNA specific; E. coli; Seawater fecal contamination; Modeling.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2675.pdf

Fecal contamination in coastal areas: An engineering approach
Provedor de dados: 5
Autores: Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf

Le bassin versant du Bélon : vers une restauration durable de la qualité bactériologique des eaux estuariennes
Provedor de dados: 5
Autores: Monfort, Patrick; Hervio Heath, Dominique; Caprais, Marie-paule; Pommepuy, Monique; Annezo, Jean-pierre; Loaec, Solen; Le Mennec, Cecile; Guillerm, Emmanuelle; Boulben, Sylviane; Bilien, Gwenael; Bonsor, Robert; Porter, Jonathan; Pickup, Roger.
Le projet européen Cycleau, initié à l'échelle locale sur la problèmatique de l'envasement et de l'ensablement de l'estuaire du Bélon, y a également intégré un volet bactériologique en raison des contraintes fortes que faisaient peser les pics conjoncturels de contamination sur la profession conchylicole (fermetures temporaires, déclassement éventuel) mais plus largement sur l'image du territoire (impact touristique) et en définitive sur son développement durable. Pour satisfaire la restauration de la qualité des eaux estuariennes, l'IFREMER a fait appel à un certain nombre d'outils susceptibles de contribuer à la formulation d'un diagnostic pertinent et ainsi de répondre aux préoccupations des acteurs du littoral. Pour ce faire, nous avons créé un réseau...
Tipo: Text
Palavras-chave: Bassin versant; Contamination fécale; Escherichia coli; Flux bactériens; Bactériophages; SIG; Évaluation.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00200/31077/29490.pdf

Relevance of Bacteroidales and F-Specific RNA Bacteriophages for Efficient Fecal Contamination Tracking at the Level of a Catchment in France
Provedor de dados: 5
Autores: Mauffret, Aourell; Caprais, Marie-paule; Gourmelon, Michele.
The relevance of three host-associated Bacteroidales markers (HF183, Rum2Bac, and Pig2Bac) and four F-specific RNA bacteriophage genogroups (FRNAPH I to IV) as microbial source tracking markers was assessed at the level of a catchment (Daoulas, France). They were monitored together with fecal indicators (Escherichia coli and enterococci) and chemophysical parameters (rainfall, temperature, salinity, pH, and turbidity) by monthly sampling over 2 years (n = 240 water samples) and one specific sampling following an accidental pig manure spillage (n = 5 samples). During the 2-year regular monitoring, levels of E. coil, enterococci, total F-specific RNA bacteriophages, and the general Bacteroidales marker AllBac were strongly correlated with one another and...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00089/20009/17680.pdf

Evaluation of viral shellfish depuration in a semi-professional tank
Provedor de dados: 5
Autores: Pommepuy, Monique; Caprais, Marie-paule; Le Saux, Jean-claude; Le Mennec, Cecile; Parnaudeau, Sylvain; Madec, Yvon; Monier, Martial; Brest, Goulven; Le Guyader, Francoise.
Depuration processes try to eliminate microorganisms using seawater to allow living, filter-feeding shellfish to naturally purge themselves from agents they accumulated from the environment. Until now the only parameter to evaluate depuration was the decrease in E. coli counts. Studies have shown that viruses can persist in the environment longer than E. coli and that shellfish meeting the endstandard (&lt; 230 E.colil /100g) have been involved in viral outbreaks. Starting in February 2000, the European Commission has been funding (Fifth Framework Programme), a 3-year project entitled "Virus Safe Seafood". One of the specific objectives of this project is to innovate technology for shellfish depuration. For this purpose a depuration system was designed and...
Tipo: Text
Palavras-chave: Bacteriophage FRNA; Depuration; Shellfish.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-1208.pdf

Accrétion océanique et déformation dans la partie méridionale du bassin Nord-Fidjien: résultats préliminaires de la campagne océanographique SEAPSO III du N.O. Jean-Charcot (décembre 1985)
Provedor de dados: 5
Autores: Auzende, Jean-marie; Eissen, Jean-philippe; Caprais, Marie-paule; Gente, Pascal; Gueneley, S; Harmegnies, Francois; Lagabrielle, Yves; Lapouille, A; Lefevre, C; Maillet, P; Maze, Jean-pierre; Ondreas, Helene; Schaaf, A; Singh, R.
Seismic, Seabeam, magnetic and gravimetrie surveys in the southern part of the N-Fiji basin were conducted during the SEAPSO LEG III Cruise. The active spreading axis was recognized between 17°30'S and 21°S. It consists of a continuous N-S Ridge cross-cut by transverse directions oriented N25 and N45. North of 17°30'S, the spreading System is more complex due to the existence of a possible triple junction located around 15° S. The eastern part of the N-Fiji basin, west of Viti Levu is mostly characterized by alternating highs and deeps (&lt;4,000 m). This area must be regarded no more as a spreading center, but as complex boundary suffering transverse tectonics.
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00184/29492/27835.pdf

Essais de décrochage de bactéries de sédiments côtiers par action chimique et physique
Provedor de dados: 5
Autores: Derrien, Annick; Dupray, Elisabeth; Caprais, Marie-paule.
Afin d'améliorer les numérations bactériennes du sédiment, nous avons testé différentes méthodes physiques et chimiques pour relarguer les bactéries des particules. Une étude préliminaire a permis de définir des concentrations et des temps d'action non toxiques pour des bactéries d'intérêt sanitaire. Les différentes techniques testées ne permettent d'augmenter la numération, dans le meilleur des cas (action du lauryl sulfate de sodium), que d'une demi unité log.
Tipo: Text
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00040/15130/12467.pdf

Etude des contaminations microbiologiques par temps sec de l'étang de Thau
Provedor de dados: 5
Autores: Serais, Ophelie; Derolez, Valerie; Caprais, Marie-paule.
Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather. Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were...
Tipo: Text
Palavras-chave: Etang de Thau; Contamination microbiologique; Contamination aviaire; E. coli; Coquillages.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00042/15337/12689.pdf

Evaluation et adaptation d'un système de purification des coquillages pour l'élimination des virus entériques
Provedor de dados: 5
Autores: Le Saux, Jean-claude; Pommepuy, Monique; Caprais, Marie-paule; Le Mennec, Cecile; Parnaudeau, Sylvain; Le Guyader, Francoise.
Un des objectifs de ce projet est d'assurer un développement économique durable à l'ostréiculture, basé sur des technologies propres à définir des produits adaptés à la sécurité alimentaire. La production conchylicole est importante sur le marché européen et représente plus d'un tiers du marché mondial, soit plus de 775 000 tonnes/an, dont plus de 140 000 tonnes d'huîtres produites par la France. En Europe, le marché des coquillages représente plus de 456 M d'euros/an produits par 8 500 entreprises employant environ 40 000 personnes. Les huîtres à elles seules, représentent plus de 140 Md' euros/an. Le développement durable de cette activité passe par la maîtrise de la qualité du produit et en particulier de sa qualité sanitaire. En fait, le libre échange...
Tipo: Text
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00044/15545/12931.pdf

Identification of the origin of faecal contamination in estuarine oysters using Bacteroidales and F-specific RNA bacteriophage markers
Provedor de dados: 5
Autores: Mieszkin, Sophie; Caprais, Marie-paule; Le Mennec, Cecile; Le Goff, Manon; Edge, T. A.; Gourmelon, Michele.
Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the...
Tipo: Text
Palavras-chave: Escherichia coli; Estuarine and river waters; F-specific RNA bacteriophages; Host-associated Bacteroidales markers; Microbial source tracking; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00153/26426/25250.pdf

Application of library-independent microbial source tracking methods for identifying the sources of faecal contamination in coastal areas
Provedor de dados: 5
Autores: Gourmelon, Michele; Caprais, Marie-paule; Le Mennec, Cecile; Mieszkin, Sophie; Ponthoreau, C.; Gendronneau, M.
Faecal contamination sources were identified in coastal areas around the Guerande-Atlantique peninsula using two microbial source tracking (MST) methods: (i) Bacteroidales host-specific 16S rRNA gene markers measured by real-time PCR and (ii) F-specific bacteriophage (FRNAPH) genotyping. Both methods were used on 63 water samples from 7 water courses. HF183 marker and bacteriophage genogroup II (FRNAPH II) were detected in all water samples and in the majority of water samples, respectively, from La Torre stream (W5), Piriac (W2), R2000 (W3) and Mazy (W7) rain water drains, and also detected, less frequently, in Le Nau drain (W4), suggesting contamination by human faecal sources at these sites. These human markers were weakly detected in Pouliguen channel...
Tipo: Text
Palavras-chave: Bacteroidales; F-specific RNA bacteriophages; Faecal bacterial indicators; Microbial source tracking; Water.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11384/10304.pdf